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Susiyanti
Universitas Sultan Ageng Tirtayasa
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RESPON PERKECAMBAHAN BENIH SINTETIK TANAMAN NILAM (Pogostemon cablin Benth.) TERHADAP PEMBERIAN KONSENTRASI ALGINAT DAN NAA SINTETIK TANAMAN Evi Megasari; Nuniek Hermita; Susiyanti Susiyanti
Jurnal Agrista Vol 24, No 1 (2020): Volume 24 Nomor 1 April 2020
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Penelitian ini dilakukan untuk mengetahui perkecambahan  benih sintetik tanaman nilam (Pogostemon cablin Benth.) dengan penambahan beberapa konsentrasi alginat dan NAA (α-Naphthaleneacetic acid). Penelitian telah dilaksanakan di Laboratorium Bioteknologi, Fakultas Pertanian, Universitas Sultan Ageng Tirtayasa pada bulan Maret - Mei 2017. Rancangan Acak Lengkap (RAL) secara faktorial digunakan dalam penelitian ini. Faktor pertama adalah konsentrasi alginat (A) dengan tiga taraf yaitu: 2% (A1), 3% (A2) dan 3% (A3). Faktor kedua adalah konsentrasi NAA (N) dengan tiga taraf yaitu 1 mg/l (N1), 2 mg/l (N2) dan 3 mg/l (N3). Dilakukan ulangan sebanyak 3 kali, sehingga diperoleh 27 satuan percobaan. Eksplan yang digunakan untuk pembuatan benih sintetik berasal dari tunas tanaman nilam hasil kultur jaringan. Hasil penelitian menunjukkan bahwa konsentrasi Alginat 4%  memberikan pengaruh yang terbaik terhadap perkecambahan benih sintetik nilam (pada parameter jumlah benih berkecambah (2,44 buah), jumlah tunas (2,67 buah) dan persentase benih berkecambah (12,78%)). Konsentrasi NAA yang diberikan tidak berpengaruh terhadap perkecambahan  benih sintetik tanaman nilam.  Demikian pula interaksi antara pemberian Alginat dan NAA tidak menunjukkan  pengaruh terhadap seluruh  peubah yang diamati. (Response germination of synthetic seeds from patchouli plants (Pogostemon Cablin Benth.) to the concentration of Alginate and NAAThis research was conducted to find out the germination of patchouli seed seeds (Pogostemon cablin Benth.) By adding some concentration of alginate and NAA (α-Naphthaleneacetic acid). The research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Sultan Ageng Tirtayasa University in March-May 2017. Completely Randomized Design (RAL) was used in this study. The first factor is the concentration of alginate (A) with three levels, namely: 2% (A1), 3% (A2) and 3% (A3). The second factor was the concentration of NAA (N) with three levels, ie 1 mg / l (N1), 2 mg / l (N2) and 3 mg / l (N3). Replication 3 times, then obtained 27 units of experiments. The explants used to make synthetic seeds are derived from patchouli shoots from tissue culture. The results showed that Alginate 4% concentration gave the best effect of germination of patchouli encapsulation.  The best response of the parameters of the number of seeds germinated (2.44 pieces), the number of shoots (2.67 pieces) and the germination percentage (12.78%)).  NAA concentration has no significant effect on synthetic seed germination. Similarly, the interaction between Alginate and NAA gave no significant effect on all observed variables
Inisiasi Tunas Kokoleceran (Vatica bantamensis) pada Berbagai Jenis Media Tanam dan Konsentrasi BAP (Benzyl Amino Purine) Secara In Vitro Sri Sudiyanti; Tubagus Bahtiar Rusbana; Susiyanti Susiyanti
Jurnal Agro Vol 4, No 1 (2017)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/1069

Abstract

Kokoleceran (Vatica bantamensis) is an endemic plant of Banten which is only in Ujung Kulon, and has been designated as identity of Banten province. Now the existence of Kokoleceran has been endangered. Based on data from the IUCN, since 1998 there has been no research. Kokoleceran initiation needs technology for preventing from the extinction. One of the mass propagation is through the technique culture in vitro. This research aimed to get the precise medium and BAP concentration for Kokoleceran, and knowing the response of Kokoleceran growth in in vitro. This research was done in November 2015 until March 2016 at the Laboratory of Biotechnology, Department of Agroecotechnology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa, Serang, Banten. This research used Completely Randomized Design (CRD) two factors. The first factor was medium that were MS and WPM media. The second factor was BAP concentration which consisted of four levels namely 0 mg L-1, 1 mg L-1, 2 mg L-1, and 3 mg L-1. The results showed that the use of different media and BAP concentrations had no impact on the time appear of shoot, shoot number, and root number. There was effect from both treatments on medium color, and growing of callus on the explants.
KARAKTERISASI BATANG DAN DAUN TANAMAN AREN DI KABUPATEN PANDEGLANG, SERANG, DAN LEBAK Yusi Meilani; Nurmayulis Nurmayulis; Susiyanti Susiyanti
Jurnal Agroekoteknologi Vol 11, No 1 (2019)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotek.v11i1.7624

Abstract

ABSTRACTSugar Palm (Arenga pinnata Merr,) has a high economic value and has the potential to be exported and can be processed into a variety of food and non-food products. Sugar palm is a multipurpose tree because all parts of the tree can be used, such as stems, palm sap, leaves. Characterization of local palm trees had been carried out in Pandegelang, Serang, and Lebak Regencies (Banten Province) which were used as making nursery material to make better sugar palm in the future (superior varieties). This study used a survey method based on guidelines for plantation germplasm management from the Indonesian Plantation Research and Development Center. The results showed the diversity of stems and leaves of palm trees in the three districts. The diversity of stem phenotypes on sugar palm was seen in the height and circumference of the stem. Diverse appearance of palm leaves seen in the length and circumference of the ptiole, the length of the rachis, the length and width of the leaflets. The study conclude that the accessions used in this study showed variations in the appearance of stems and leaves from the accessions of palm trees observed.
PENGARUH UMUR BATANG BAWAH DAN NAUNGAN TERHADAP PERTUMBUHAN SAMBUNG DINI DURIAN (Durio zhibertinus L.) Rama Robbi Ghifari; Susiyanti; Julio Eiffelt Rossaffelt Rumbiak; Alfu Laila
Gunung Djati Conference Series Vol. 33 (2023): Seminar Nasional Pertanian 2023
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

This study aims to determine the effect of rootstock age and shade on early grafting growth of durian (Durio zibethinus L.). The environmental design used in this study was using a Split Plot with the main plot being the effect of shading and the subplot being the age of the rootstock which was replicated 3 times. The treatment design consisted of a main plot and subplots. The main plot is the effect of shading (N) with 3 different light intensity levels : N1: 40% light intensity N2: 60% light intensity N3: 80% light intensity. Subplots were the effect of rootstock age (U) which consisted of 3 levels, namely: U1: Age of rootstock 2 weeks. U2 : Age of rootstock 4 weeks. U3 : Age of rootstock 6 weeks. The results showed that the 4-week rootstock age treatment had the best effect on the parameters of the number of open leaves, leaf greenness index; increase in scion diameter at 1-8 (week after grafting/WAG), and increase in rootstock diameter at 8 MSP; Shading treatment of 60% had the best effect on the parameters of shoot rupture age, number of open leaves, leaf greenness index, shoot length increase at 4-8 WAG; The best interactions occurred at 60% shade treatment and 6 weeks of rootstock age on the parameters of bud break age and leaf greenness index; Shade treatment 60% and rootstock age 8 weeks on parameters Number of open leaves, and increase in shoot length; Shade treatment of 80% and rootstock age of 8 weeks for the parameters of scion diameter increase; and 40% shading treatment and rootstock age of 6 weeks for the parameter of rootstock diameter increase.
INDUKSI TUNAS PADA DUA VARIETAS PISANG (Musa acuminata C.) TERHADAP LAMA PENGGELAPAN SECARA IN VITRO Dzikrie Mary Ainipasha; Susiyanti Susiyanti; Sulastri Isminingsih; Zahratul Millah
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 49, No 1 (2024)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v49i1.13485

Abstract

A sufficient supply of healthy plants must be provided to balance the rising demand for bananas. A technique that can be used for propagation is tissue culture. Environmental factors and a variety of types are factors that affect explant growth. This research aimed to determine the effect of darkening duration on the growth of two banana (Musa sp.) varieties in vitro. In this study, a Factorial Randomized Block Design was implemented, considering two elements, namely the variety of bananas and the duration of darkening. The research showed that the time of shoot emergence started at 1.83 WAP in Cavendish grand naine treatment without darkening. The type of variety treatment had a significant effect on the number of leaves, and the best variety was Cavendish grand naine. The darkening duration treatment with four weeks of darkening increased shoot height significantly. An interaction between these two factors was observed in the number of shoots, shoot height, and number of leaves.
MULTIPLIKASI TUNAS PADA DUA VARIETAS PISANG (Musa acuminata L.) DENGAN PEMBERIAN BEBERAPA KONSENTRASI SITOKININ Ayna, Qorry; Isminingsih, Sulastri; Susiyanti, Susiyanti; Yenny, Ratna Fitry
Jurnal Agroekoteknologi Vol 15, No 2 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i2.22348

Abstract

This study aimed to investigate how the application of various cytokinin concentrations affects the shoot multiplication in two varieties of banana (Musa acuminata L.). The research was conducted at the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sultan Ageng Tirtayasa University. The study was designed as a randomized complete block design, consisting of two factors. The first factor included two types of banana varieties: bagja (v1) and cavendish (v2). The second factor included six types of application of various concentrations of cytokinin, which were 1 mg/l BAP (k1), 2 mg/l BAP (k2), 3 mg/l BAP (k3), 1 mg/l kinetin (k4), 2 mg/l kinetin (k5), and 3 mg/l kinetin (k6). The results showed a significant difference in the effect of cavendish banana varieties explant on the height of the plant, with an average value of 3.26 cm. The application of various concentrations of cytokinin didn’t significantly affect the parameters of the height of the plant, shoot emergence time, number of shoots, and time of root emergence.Keywords: Bagja, Cavendish, Cytokinin, In Vitro Culture
Pengaruh Intensitas Cahaya terhadap Jumlah Klorofil dan Laju Fotosintesis pada Berbagai Usia Bibit Kelapa Sawit (Elaeis Guinensis Jaqc) Fatmawaty, Andi Apriany; Nurmayulis, Nurmayulis Nurmayulis; Susiyanti, Susiyanti Susiyanti; Sodiq, Abdul Hasyim
Jurnal Agroekoteknologi Vol 16, No 2 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i2.22363

Abstract

This research aimed to determine the effects of different light intensities and seedling ages on oil palm (Elaeis guinensis Jacq.) nurseries. This research was an experimental type of research carried out from May to August 2023 at the experimental garden of the Faculty of Agriculture, University of Sultan Ageng Tirtayasa, Serang Regency, Banten Province. This research used a factorial split plot design, which consists of two factors. The first factor was the difference in light intensity, which consists of 3 levels: no shade (N0), 20% net shade (N1), and 40% net shade (N2). The second factor or subplot was the age of oil palm seeds, which consists of 3 levels: 3 months of age (B1), 6 months of age (B2), and 9 months of age (B3). The results of the research showed that there was an interaction between light intensity treatment and seedling age that was significantly different in the 1000 nm wave assimilation parameter. The independent influence of light intensity treatment and seedling age occurred on the total chlorophyll parameter. The independent effect of seedling age treatment occurred on the assimilation and evapotranspiration parameters at 1000 nm waves. 
Co-Authors Alfu Laila Ayna, Qorry Dzikrie Mary Ainipasha Evi Megasari Fatmawaty, Andi Apriany Isminingsih, Sulastri Julio Eiffelt Rossaffelt Rumbiak Nuniek Hermita Nurmayulis Rama Robbi Ghifari Sodiq, Abdul Hasyim Sri Sudiyanti Sulastri Isminingsih Tubagus Bahtiar Rusbana Yenny, Ratna Fitry Yusi Meilani Zahratul Millah