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IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN RINI SUNDARI; IDA PARWATI; JOHANES C. MOSE; BUDI SETIABUDIAWAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.

Laporan Kasus: Penyakit Kawasaki Atipikal Budi Setiabudiawan; Reni Ghrahani; Gartika Sapartini; Mohamad Yanuar Anggara; Herry Garna
Majalah Kedokteran Bandung Vol 43, No 3
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Penyakit Kawasaki merupakan penyebab utama kelainan jantung dapatan yang sering ditemukan pada anak. Di Indonesia, penyakit ini masih sangat jarang didiagnosis karena dianggap masih jarang dan belum diketahui secara luas. Dua laporan kasus berikut merupakan laporan kasus anak perempuan dan laki-laki, masing-masing berusia 17 bulan dan 3 tahun. Keduanya datang dengan demam yang persisten lebih dari 5 hari dan hanya memenuhi 3 kriteria klasik penyakit Kawasaki, yakni mata merah dan disertai dengan perubahan mukosa bibir serta ekstremitas. Penderita kemudian didiagnosis sebagai penyakit Kawasaki atipikal. Pada pemeriksaan laboratorium didapatkan peningkatan C-reactive protein dan laju endap darah disertai gambaran ekokardiografi yang normal. Kedua anak diberikan imunoglobulin intravena (IGIV) dengan dosis 2 gram/kgBB dosis tunggal dan aspirin dosis 80 mg/kgBB/hari. Penderita mengalami perbaikan setelah 1 hari mendapat terapi kombinasi tersebut. Disimpulkan bahwa pengobatan dengan kombinasi IGIV dan aspirin memberikan respons yang baik pada penyakit Kawasaki atipikal. [MKB. 2011;43(3):146–52].Kata kunci: Aspirin, imunoglobulin intravena, penyakit Kawasaki atipikal Case Reports: Atypical Kawasaki DiseaseKawasaki disease is the most common cause of acquired heart disease in children. In Indonesia the disease is rare to diagnosed, because of difficulty in diagnosis and not widely known. These were 2 case reports about a girl and a boy age 17 months and 3 years, who came with persistent fever more than 5 days and only fulfilled 3 criteria of Kawasaki disease, which are red eyes, changes in lips, mucose of oral and extremities. They were diagnosed as atypical Kawasaki disease. Laboratory examinations showed an increased of C-reactive protein and erythrocyte sedimentation rate with normal echocardiography. The patients were improved after treated with 2 grams per bodyweight of intravenous immunoglobulin (IVIG) and 80 mg per bodyweight of aspirin. The patients were better after one day combination therapy. In conclusion that atypical Kawasaki disease has good response to combination of IVIG and aspirin. [MKB. 2011;43(3):146–52].Key words: Aspirin, atypical Kawasaki disease, intravenous immunoglobulin DOI: http://dx.doi.org/10.15395/mkb.v43n3.61

The association between fever in the first year of life and atopy in children with or without family history of atopic disease Susy P. Wihadi; Budi Setiabudiawan; Cissy B. Kartasasmita
Paediatrica Indonesiana Vol 47 No 2 (2007): March 2007
Publisher : Indonesian Pediatric Society

Background The role of repeated infection in early life in thedevelopment of childhood atopy is still controversy. Fever in thefirst year of life which is frequently associated with infections mightdecrease atopy.Objective The aim of this study was to investigate the associationbetween fever in the first year of life and atopy in children.Methods This was an observational clinical epidemiology studyperformed at Puskesmas Garuda, Padasuka, and Babakan Sari,Bandung, from January to March 2006. From 749 children, werandomly chose 150 subjects each from group with and withoutfamily history of atopic disease. Skin prick test and measurementof total serum immunoglobulin (Ig) E were performed on eachchildren. Atopy was defined as the skin prick test result waspositive to >1 allergen. The number of fever episodes in the firstyear of life was based on parents report. The relationship betweenfever and atopy was analyzed using Mantel Haenszel.Results From 284 subjects, atopy was found in 28.2% of children,of which 32.4% with and 23.9% without a family history of atopicdisease. Generally there was no significant association betweenfever and atopy. There was only decreased odds ratio withincreased fever episodes and trend analysis showed this decreasewas significant (P=0.01). The significant association betweenfever and atopy were found only in group without family historyof atopic disease (P=0.03, OR=0.43, CI 95% 0.18;1.01).Conclusion There is a relationship between fever and atopy inchildren without family history of atopic disease.

The association between duration of breastfeeding and atopy in children with or without family history of atopic disease Riana Novy; Budi Setiabudiawan; Cissy B. Kartasasmita
Paediatrica Indonesiana Vol 47 No 4 (2007): July 2007
Publisher : Indonesian Pediatric Society

Background Atopic diseases (AD) are the most common chronicdiseases in childhood, and their incidence has a tendency to increaserecently. Tendency to have atopy could be triggered by many factorsoriginated in early life, including time of breastfeeding cessation.Objective To determine the association between exclusive andduration of breastfeeding and atopy in children with or withoutfamily history of atopic disease.Methods This was an observational clinical epidemiology studyperformed at Babakansari, Padasuka, Garuda Primary Health CareCenter in Bandung from January to March 2006. One hundredfifty of 749 children were randomized from group with and withoutfamily history of AD. They underwent skin prick tests and totalserum IgE level analysis. Atopy was defined as a positive skinprick test to any of the eight allergens tested. History of exclusiveand duration of breastfeeding was obtained from their parents.Significance tests for contingency tables were on the basis of x 2test for association odds ratio with 95% confidence interval.Results Atopy was found in 28.2% of children, of whom 32.4% withand 23.9% without family history of AD. Children exclusivelybreastfed exhibited a reduced risk of atopy (5.8% v 35.3%, OR=0.11,95%CI= 0.03;0.34, P<0.001). The difference of atopy was stronglysignificant between children who had exclusive breastfeeding andthose without exclusive breastfeeding whether or not the subjectshad family history of AD (P<0.001). There was a highly significantrisk reduction for atopy related to prolonged breastfeeding (=6months) (OR=0.37, 95%CI = 0.19 to 0.72, P=0.001). Thedifference of atopy was strongly significant between children whohad prolonged breastfeeding and short breastfeeding duration whetheror not the subjects had family history of AD (P<0.001)Conclusions Exclusive and prolonged breastfeeding decrease atopyin children with as well as without family history of AD.

Relationship between atopic manifestations, family history of atopic disease and cord blood IgE levels in children Tisnasari Hafsah; Myrna Soepriadi; Budi Setiabudiawan; Herry Garna
Paediatrica Indonesiana Vol 47 No 6 (2007): November 2007
Publisher : Indonesian Pediatric Society

Background The incidence of atopic disease tends to increaseover the past few decades and its morbidity interferes with thequality of life and health. Prediction of the disease is importantfor early prevention.Objective To evaluate the relationship between atopicmanifestations, family history (FH) of atopic disease and cordblood IgE (CB-IgE) levels.Methods We conducted an analytic observational study withcohort retrospective design on children with an average age of 3years whose CB-IgE had been measured at delivery inKiaracondong Primary Health Care during Octoberâ€“December2004. Manifestations of atopic disease were recorded using ISAACquestionaire for allergy. Chi-square, Mann-Whitney test, andlogistic regression analysis were used for analysis.Results Cord blood IgE was measured on 124 children after birth.Only 94 children (76%) fulfilled the inclusion criteria. Atopicdisease was found in 17 children (18%), consisting of 8 childrenwith atopic dermatitis, 4 with allergic rhinitis, and 5 suffered fromboth. There were significant differences in the mean value of CB-IgE (Z M-W =4.60; P<0.001) and FH (x 2 =19.059; P<0.001)between atopic and non atopic children. Cut off point of the CB-IgE concentration was 1.4 IU/mL (77.7%). The highest probabilityfor atopic manifestations was found in children who had highCB-IgE and positive FH (P=45%). Relative risk of children withhigh CB-IgE level in positive FH group was 3.636 (95% CI0.943;14.016).Conclusion CB-IgE level and family history of atopic disease arerisk factors for the development of atopic manifestation.

The association between onset, frequency, duration of seizure and IQ level in epileptic children Yulia Yulia; Sjarif Hidayat Effendi; Budi Setiabudiawan
Paediatrica Indonesiana Vol 49 No 3 (2009): May 2009
Publisher : Indonesian Pediatric Society

Background Epilepsy is a common neurological disorder found inall races and age groups. Epilepsy becomes a serious problem when occurs during the child's critical development period. It is known that onset, frequency, and duration of seizures are associated with IQ level. Therefore, intelligent assessment is important to determine prognostic and holistic management.Objective To determine the association between onset, frequency, duration of seizure and IQ level in epileptic children.Methods This cross sectional study was carried out at theDepartment of Child Health, Hasan Sadikin Hospital, Bandung,Indonesia from October to December 2007. The subjects wereepileptic children aged 4-16 years old being treated with valproicacid. Statistic analysis was done using logistic regression analysis, OR and RR, with 95% confidence interval.Results There were 90 subjects with epilepsy (46 males). Twentysubjects (22%) showed onset of seizures at < 18 months old; these subjects had 3.08 higher risk for having a low IQ level (score <90) compared to those with seizure onset at~ 18 months old (P=0.003). Sixty subjects (67%) had a seizure frequency of> 10 times annually; they had 1.68 higher risk of having a low IQcompared to those with seizure frequency< 10 times (P=0.430).Seven subjects (28.0%) had seizures of> 10 minutes; they had 1.17 higher risk of having a low IQ compared to those with seizures of < 10 minutes (P=0.706).Conclusion Onset of seizures at < 18 months old is significantlyassociated with low IQ level, while frequency and durationof seizure have no significant association with lower IQ level.

Association between serum vitamin D level and tuberculosis in children Ahmad Zaeni Syafii; Abdurachman Sukadi; Budi Setiabudiawan
Paediatrica Indonesiana Vol 48 No 6 (2008): November 2008
Publisher : Indonesian Pediatric Society

Background A possible association between vitamin 0 andtuberculosis has been described. In adult, vitamin 0 is consideredto have a role in protecting tuberculosis. On the other hand,tuberculosis infection can decrease serum vitamin 0 level.Objective To find out the difference between serum vitamin 0level in children with and without tuberculosis, and to find theassociation of serum vitamin 0 level with tuberculosis.Methods A cross sectional study was conducted in Cibabat Hospital,Ban dung from July to October 2007. We selected children :S 14years, diagnosed as tuberculosis, and had positive response aftertwo month treatment; for control we selected randomly siblingsor neighbors who didn't have tuberculosis. We excluded childrenwith liver abnormalities and immunocompromized children.Mann-Whitney test and OR method with 95% confidence intervalwas used to analyze the data.Results Thirty-nine children with tuberculosis (21 boys, 18 girls)and 39 children without tuberculosis (19 boys, 20 girls) as wereenrolled. Mean serum vitamin 0 level of children with and withoutTB were 4 7 (SO 25) pmol/L and 125 (SO 3 7) pmol/L, respectively(P=O.OOl). All children without tuberculosis had normal vitamin0 level while of those with tuberculosis, 14 children had normallevel and 25 children were deficient (corrected OR: 139, 95%CI8 to 238).Conclusion Serum vitamin 0 level is low in children withtuberculosis.

Association between immunization coverage and atopy in children with or without family history of atopic disease Isabella Riandani; Budi Setiabudiawan; Cissy B. Kartasasmita
Paediatrica Indonesiana Vol 48 No 6 (2008): November 2008
Publisher : Indonesian Pediatric Society

Background Atopic diseases are determined by the interactionbetween genetic and environmental factors. The possible effectsof immunization, as one of environmental factors, on atopy remaina matter of controversy.Objective We conducted an observational clinical epidemiologyto find out the protective effect of high vaccination coverage toatopy in children.Methods During January through March 2006, 150 of749 childrenat Garuda, Padasuka, and Babakan Sari Primary Health Care inBandung were randomized from group with and without familyhistory of atopic disease. Atopy derived from skin prick test andtotal serum lgE was evaluated. Atopy was defined as a positiveskin test to any of the eight allergens tested. The immunizationswere recorded from Kartu Menuju Sehat (KMS). Statistical analysesincluded Chi square to compare prevalence, independent T-testand Mann-Whitney to compare mean.Results Atopy was found in 28.2% of284 subjects, of which 32.4%with and 23.9% without a family history of atopic disease. Themedian of total serum lgE level was higher in children with familyhistory of atopic disease and in atopy children. Children weregrouped according to total dose of basic immunizations (0-17 and2: 18) based on Program Pengembangan Imunisasi (PPI). There wasnonsignificant association between total doses of immunizationand atopy. Even though no statistically significant, the cumulativeimmunization doses were inversely related to the median of totalserum IgE level.Conclusions The immunization coverage has not decreased atopyrisk.

Association between cord blood IgE levels in newborns and family history of atopic diseases Andhika T. Hutapea; Budi Setiabudiawan; Myrna Soepriadi; Diet Sadiah Rustama
Paediatrica Indonesiana Vol 46 No 5 (2006): September 2006
Publisher : Indonesian Pediatric Society

Background Cord blood-IgE (CB-IgE) levels have been usedwidely as a specific marker of atopic diseases. In some previousstudies, CB-IgE levels in subjects with and without a family historyof atopic diseases have been controversial.Objective To determine the CB-IgE level in newborns and to iden-tify the association between CB-IgE and family history of atopicdiseases.Methods A cross-sectional study was done to compare the CB-IgElevels in neonates with or without a family history of atopic diseasesin mother, father, or siblings. Subjects of this study were 124 new-borns who consecutively born in Puskesmas Kiaracondong,Bandung, during the period of March 2001 to July 2002. Subjectswere divided into 2 groups based on history of atopic diseases.Measurements of CB-IgE levels were done by sandwich ELISAmethods. Data were analyzed by c 2 statistics, t test, ANOVA, andDunkanâ€™s test.Results The mean CB-IgE levels in the group with and without afamily history of atopic diseases were 3.2Â±2.5 IU/ml and 0.5Â±0.5IU/ml (P<0.001), respectively. The mean CB-IgE levels in maleand female infants with a family history of atopic diseases were3.3Â±2.7 IU/ml and 3.03Â±2.2 IU/ml (P>0.05), respectively. Basedon the cut-off point (1.3 IU/ml), CB-IgE levels had significant posi-tive association with a family history of atopic diseases (OR 156,95%CI 29.61;1104.24). CB-IgE levels in neonates with 1, 2, and 3atopic family members were 1.67Â±0.78 IU/ml, 3.76Â±2.11 IU/ml, and6.6Â±2.7 IU/ml, respectively (F=32.603; P<0.001).Conclusion Most newborns with a family history of atopic dis-eases showed high levels of CB-IgE, but there were no correlationwith gender. The probability of having atopic diseases increase inconcord with the number of family with atopic diseases

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