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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
S Hastiono
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Control of Gastrointestinal Nematodes Infection in Small Ruminants by Nematophagous Fungi Ahmad, Riza Zainuddin; ., Beriajaya; Hastiono, S
Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.129 KB) | DOI: 10.14334/wartazoa.v12i3.765

Abstract

The disease caused by gastrointestinal nematodes infection in small ruminants in Indonesia is quite unprofitable. Control with anthelmintic developed a negative effect of anthelmintic resistance and residue in tissue if anthelmintic are given routinely. Biological control is one of the control methods using microorganisms especially molds. The moulds can form traps and destroy the body of larvae and eggs by hife and produce toxins. Few isolates of nematophagous fungi such as Arthrobotrys spp. and Monacrosporium spp. were found from surveys done in West Java. Applications of these fungi as biological control against nematodiasis is promising as indicated from in vitro and in vivo studies.   Key words: Biological control, nematophagous fungi, small ruminants
Selection of phytase-producing moulds Susana, I.W.R; Tangenjaya, B; Hastiono, S
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.39 KB) | DOI: 10.14334/jitv.v5i2.207

Abstract

Organic phosphorous in the form of phytate salts are found in feed component originated from cereals which can not be digested by chicken unless after the addition of an enzyme, i.e. phytase. A research to investigate phytase-producing moulds had been carried out. A total of 60 isolates from various collection units and isolation from samples had been collected then screened in Czapek’s medium with Ca-phytate as the source of phosphorous. From mould growth measurements and clearing zones formation there were 4 mould isolates, i.e. Aspergillus ficuum NRRL 3135 and NRRL 320, Aspergillus niger M94/18/D2 (Balitvet isolate), and Aspergillus niger no. 58 (IPB isolate), which were further observed. Enzyme was produced from medium containing 8% of corn’s starch, 3% of glucose, 0.0005% of KCl and MgSO4.7H2O, 0.0002% of K2HPO4, and 0.00018% of FeSO4.7H2O. The enzyme activity, protein and biomass contents were observed at 16, 24, 32, 48 and 72 hours incubations for Aspergillus niger and at 1, 2, 3, 4 and 5 days incubations for Aspergillus ficuum. The highest protein content was found at 5 days incubation for Aspergillus ficuum NRRL 3135 and NRRL 320, (165 and 169 μg/ml respectively), while for Aspergillus niger (Balitvet and IPB isolates) at 16 hours incubation (102 and 112 μg/ml respectively). Meanwhile, the biomass contents were decreased in accordance with the duration of incubations. The maximum enzyme productions were reached at 24 hours incubation for Aspergillus niger and at 4 days incubation for Aspergillus ficuum. The highest phytase activities were reached at 2.808 U/ml for Aspergillus ficuum NRRL 3135, followed by 1.520 U/ml for Aspergillus ficuum NRRL 320. Aspergillus niger produced enzyme quicker than the other moulds, with lower activity level and decreases quicker at the following hours.   Key words: Phytate, phytase, Aspergillus ficuum, Aspergillus niger
Selection of phytase-producing moulds I.W.R Susana; B Tangenjaya; S Hastiono
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.39 KB) | DOI: 10.14334/jitv.v5i2.207

Abstract

Organic phosphorous in the form of phytate salts are found in feed component originated from cereals which can not be digested by chicken unless after the addition of an enzyme, i.e. phytase. A research to investigate phytase-producing moulds had been carried out. A total of 60 isolates from various collection units and isolation from samples had been collected then screened in Czapek’s medium with Ca-phytate as the source of phosphorous. From mould growth measurements and clearing zones formation there were 4 mould isolates, i.e. Aspergillus ficuum NRRL 3135 and NRRL 320, Aspergillus niger M94/18/D2 (Balitvet isolate), and Aspergillus niger no. 58 (IPB isolate), which were further observed. Enzyme was produced from medium containing 8% of corn’s starch, 3% of glucose, 0.0005% of KCl and MgSO4.7H2O, 0.0002% of K2HPO4, and 0.00018% of FeSO4.7H2O. The enzyme activity, protein and biomass contents were observed at 16, 24, 32, 48 and 72 hours incubations for Aspergillus niger and at 1, 2, 3, 4 and 5 days incubations for Aspergillus ficuum. The highest protein content was found at 5 days incubation for Aspergillus ficuum NRRL 3135 and NRRL 320, (165 and 169 μg/ml respectively), while for Aspergillus niger (Balitvet and IPB isolates) at 16 hours incubation (102 and 112 μg/ml respectively). Meanwhile, the biomass contents were decreased in accordance with the duration of incubations. The maximum enzyme productions were reached at 24 hours incubation for Aspergillus niger and at 4 days incubation for Aspergillus ficuum. The highest phytase activities were reached at 2.808 U/ml for Aspergillus ficuum NRRL 3135, followed by 1.520 U/ml for Aspergillus ficuum NRRL 320. Aspergillus niger produced enzyme quicker than the other moulds, with lower activity level and decreases quicker at the following hours.   Key words: Phytate, phytase, Aspergillus ficuum, Aspergillus niger
Control of Gastrointestinal Nematodes Infection in Small Ruminants by Nematophagous Fungi Riza Zainuddin Ahmad; Beriajaya .; S Hastiono
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 3 (2002)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.129 KB) | DOI: 10.14334/wartazoa.v12i3.765

Abstract

The disease caused by gastrointestinal nematodes infection in small ruminants in Indonesia is quite unprofitable. Control with anthelmintic developed a negative effect of anthelmintic resistance and residue in tissue if anthelmintic are given routinely. Biological control is one of the control methods using microorganisms especially molds. The moulds can form traps and destroy the body of larvae and eggs by hife and produce toxins. Few isolates of nematophagous fungi such as Arthrobotrys spp. and Monacrosporium spp. were found from surveys done in West Java. Applications of these fungi as biological control against nematodiasis is promising as indicated from in vitro and in vivo studies.   Key words: Biological control, nematophagous fungi, small ruminants
Co-Authors B Tangenjaya Beriajaya . I.W.R Susana Riza Zainuddin Ahmad Riza Zainuddin Ahmad