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Influence of feeding protected protein and energy on intake and digestion by lambs Mathius, I-W; Haryanto, B; Susana, I.W.R
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 2 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.624 KB) | DOI: 10.14334/jitv.v3i2.100

Abstract

To determine the effect of additional protected protein and energy on intake and digestion in lambs, a trial was conducted using 36 young sheep (average body weight 24 .8 _+ 1 .7 kg) in a completely randomized design . Combination of three levels of protected protein (0, 10 and 20%, based on the dry matter of the concentrate offered) and three levels of protected energy (0, 5, 10%, based on the dry matter of the concentrate offered) were added into concentrate diet and fed to growing sheep. Results showed that different levels of feed additive did not affect (P>0 .05) intake of dry matter, organic matter, neutral detergent fiber, acid detergent fiber and metabolizable energy, with average of 76 .3 g, 68.9 g, 31 .2 g, 16 .79 g and 0.75 MJ /kg BW0.75 respectively . Level of protected protein gave significant (P<0 .05) respons on crude protein intake (7 .4 vs 8.7 vs 9.8 g/kg BW0.75) . Combination of protected protein and energy did not affect animal ability to digest the nutrient effectively. A mixtures of 20 % protected protein and 5 %energy addition into concentrate diet increased average daily gain (ADG) as much as 100 g. It was also found that for every gram ADG needs 42.2 kJ metabolizable energy .   Key words : Protein, protected energy, sheep
Selection of phytase-producing moulds Susana, I.W.R; Tangenjaya, B; Hastiono, S
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.39 KB) | DOI: 10.14334/jitv.v5i2.207

Abstract

Organic phosphorous in the form of phytate salts are found in feed component originated from cereals which can not be digested by chicken unless after the addition of an enzyme, i.e. phytase. A research to investigate phytase-producing moulds had been carried out. A total of 60 isolates from various collection units and isolation from samples had been collected then screened in Czapek’s medium with Ca-phytate as the source of phosphorous. From mould growth measurements and clearing zones formation there were 4 mould isolates, i.e. Aspergillus ficuum NRRL 3135 and NRRL 320, Aspergillus niger M94/18/D2 (Balitvet isolate), and Aspergillus niger no. 58 (IPB isolate), which were further observed. Enzyme was produced from medium containing 8% of corn’s starch, 3% of glucose, 0.0005% of KCl and MgSO4.7H2O, 0.0002% of K2HPO4, and 0.00018% of FeSO4.7H2O. The enzyme activity, protein and biomass contents were observed at 16, 24, 32, 48 and 72 hours incubations for Aspergillus niger and at 1, 2, 3, 4 and 5 days incubations for Aspergillus ficuum. The highest protein content was found at 5 days incubation for Aspergillus ficuum NRRL 3135 and NRRL 320, (165 and 169 μg/ml respectively), while for Aspergillus niger (Balitvet and IPB isolates) at 16 hours incubation (102 and 112 μg/ml respectively). Meanwhile, the biomass contents were decreased in accordance with the duration of incubations. The maximum enzyme productions were reached at 24 hours incubation for Aspergillus niger and at 4 days incubation for Aspergillus ficuum. The highest phytase activities were reached at 2.808 U/ml for Aspergillus ficuum NRRL 3135, followed by 1.520 U/ml for Aspergillus ficuum NRRL 320. Aspergillus niger produced enzyme quicker than the other moulds, with lower activity level and decreases quicker at the following hours.   Key words: Phytate, phytase, Aspergillus ficuum, Aspergillus niger
Influence of feeding protected protein and energy on intake and digestion by lambs I-W Mathius; B Haryanto; I.W.R Susana
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 2 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.624 KB) | DOI: 10.14334/jitv.v3i2.100

Abstract

To determine the effect of additional protected protein and energy on intake and digestion in lambs, a trial was conducted using 36 young sheep (average body weight 24 .8 _+ 1 .7 kg) in a completely randomized design . Combination of three levels of protected protein (0, 10 and 20%, based on the dry matter of the concentrate offered) and three levels of protected energy (0, 5, 10%, based on the dry matter of the concentrate offered) were added into concentrate diet and fed to growing sheep. Results showed that different levels of feed additive did not affect (P>0 .05) intake of dry matter, organic matter, neutral detergent fiber, acid detergent fiber and metabolizable energy, with average of 76 .3 g, 68.9 g, 31 .2 g, 16 .79 g and 0.75 MJ /kg BW0.75 respectively . Level of protected protein gave significant (P<0 .05) respons on crude protein intake (7 .4 vs 8.7 vs 9.8 g/kg BW0.75) . Combination of protected protein and energy did not affect animal ability to digest the nutrient effectively. A mixtures of 20 % protected protein and 5 %energy addition into concentrate diet increased average daily gain (ADG) as much as 100 g. It was also found that for every gram ADG needs 42.2 kJ metabolizable energy .   Key words : Protein, protected energy, sheep
Selection of phytase-producing moulds I.W.R Susana; B Tangenjaya; S Hastiono
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.39 KB) | DOI: 10.14334/jitv.v5i2.207

Abstract

Organic phosphorous in the form of phytate salts are found in feed component originated from cereals which can not be digested by chicken unless after the addition of an enzyme, i.e. phytase. A research to investigate phytase-producing moulds had been carried out. A total of 60 isolates from various collection units and isolation from samples had been collected then screened in Czapek’s medium with Ca-phytate as the source of phosphorous. From mould growth measurements and clearing zones formation there were 4 mould isolates, i.e. Aspergillus ficuum NRRL 3135 and NRRL 320, Aspergillus niger M94/18/D2 (Balitvet isolate), and Aspergillus niger no. 58 (IPB isolate), which were further observed. Enzyme was produced from medium containing 8% of corn’s starch, 3% of glucose, 0.0005% of KCl and MgSO4.7H2O, 0.0002% of K2HPO4, and 0.00018% of FeSO4.7H2O. The enzyme activity, protein and biomass contents were observed at 16, 24, 32, 48 and 72 hours incubations for Aspergillus niger and at 1, 2, 3, 4 and 5 days incubations for Aspergillus ficuum. The highest protein content was found at 5 days incubation for Aspergillus ficuum NRRL 3135 and NRRL 320, (165 and 169 μg/ml respectively), while for Aspergillus niger (Balitvet and IPB isolates) at 16 hours incubation (102 and 112 μg/ml respectively). Meanwhile, the biomass contents were decreased in accordance with the duration of incubations. The maximum enzyme productions were reached at 24 hours incubation for Aspergillus niger and at 4 days incubation for Aspergillus ficuum. The highest phytase activities were reached at 2.808 U/ml for Aspergillus ficuum NRRL 3135, followed by 1.520 U/ml for Aspergillus ficuum NRRL 320. Aspergillus niger produced enzyme quicker than the other moulds, with lower activity level and decreases quicker at the following hours.   Key words: Phytate, phytase, Aspergillus ficuum, Aspergillus niger
Co-Authors B Haryanto B Tangenjaya I-W Mathius S Hastiono