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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika Natural B
Sri Rahayu
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : Natural B

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Identification of GDF-9 and BMP-15 Gene Polymorphisms on Goat Beans Sri Rahayu
Natural B, Journal of Health and Environmental Sciences Vol 1, No 2 (2011)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.358 KB) | DOI: 10.21776/ub.natural-b.2011.001.02.4

Abstract

The aim of this research was to determine the polymorphisms of BMP-15  and GDF-9 gene of  Kacang goat. DNA was isolated from blood samples of five female goats with salting out method. Quantitative and qualitative  analysis of DNA was measured using spectrophotometer and agarose gel electrophoresis. To get DNA fragment GDF-9 gene was amplified using Forward 5'-AGTTTGTACTGAGCAGGTCT -3 ', Reverse 5'-ACTCCGCTTCGTATGTCAG-3', while to get  DNA fragmen BMP-15 gene was amplified using Forward primer 5’-CAAGGAGGGGACCCCTAAAT-3’, reverse primer 5’- ACCAGAGGCTCAAGAGGAGC-3 for GDF-9. The results of amplification was a specific DNA band with the length of 1400 bp for GDF-9 gene  and 400 bp for BMP-15 gene. Restriction with HaeIII enzyme of GDF-9 PCR product was resulted a haplotype with fragment size 1100 bp, 300 bp, and 100 bp. While, restriction with AluI enzymes of BMP-15 PCR product was resulted a haplotype which consist of two fragments with sizes 180 bp and 120 bp. It was concluded that there are no polymorphism of GDF-9 and BMP-15 gene of Goat Beans.
Co-Authors Agung Pramana W.M Agung Pramana Warih Marhendra Apriani Herni Rophi Aris Soewondo Bella Pradina Novinda Wardani Choirul Anam Gatot Ciptadi Intan Sartika Risky Sampurna Ivakhul Anzila Jantje Wiliem Souhaly Khairatul Insani Febrianti Moch Sasmito Djati Mochammad Dwi Susan Muhaimin Rifa'i Muhammad Basyaruddin Riska Annisa Rosyi Wirastuti