Article Per Year (5 Year)
p-Index From 2021 - 2026
1.503
P-Index
This Author published in this journals
All Journal Jurnal Sains dan Seni ITS Biotropika Jurnal Biosilampari: Jurnal Biologi Evolusi: Journal of Mathematics and Natural Sciences Proceeding Biology Education Conference Komatika: Jurnal Pengabdian Kepada Masyarakat Bioconsortium : Biological Research and Education ABDISUCI: Jurnal Pengabdian dan Pemberdayaan Masyarakat
Hefdiyah Hefdiyah
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

Published : 10 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Biotropika

 1 
Potential of Plant Growth-Promoting Rhizobacteria (PGPR) from The Tobacco Rhizosphere in Prancak Village to Degrade Organic Matter Hefdiyah, Hefdiyah; Ach. Kholish; Safitri, Soviana Fila
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.07

Abstract

Organic agriculture is growing in Sumenep due to heightened awareness of the negative impacts of synthetic chemical use. Utilising Plant Growth-Promoting Rhizobacteria (PGPR) from the rhizosphere of tobacco plants in Prancak Village presents an alternative to chemical fertilisers and pesticides. It may serve as a biostimulant, encouraging farmer independence and maintaining the organic farming practices. This study examined the diversity of bacteria in the rhizosphere of tobacco plants in Prancak Village. It also examined the efficiency of nitrogen-fixing and phosphate-solubilising bacteria, or PGPR, in decomposing organic matter. The bacterial population was measured using the total plate count method. The soil physicochemical factors considered included pH, texture, and colour, while the potential for organic matter degradation was assessed of measuring inhibition zones. The results showed that the PGPR population in the tobacco rhizosphere ranged from 0.83 to 6.68 × 106 CFU. Nine isolates of potential organic matter-degrading bacteria were identified, with index values for the Amylolytic Index (AI) ranging from 16.7 mm to 42.8 mm, Cellulolytic Index (CI) from 68 cm to 4.31 cm, Proteolytic Index (PI) from 2.05 mm to 5.94 mm, and Lipolytic Index (LI) from 1.62 mm to 5.20 mm.
Isolation and Identification of Non-Symbiotic Nitrogen-Fixing Bacteria in Prancak Village Tobacco Farming Soil Hefdiyah, Hefdiyah; Rofiqoh, Rofiqoh
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.04

Abstract

Prancak 95 is one of the most prominent tobacco (Nicotiana tabacum L.) varieties in Indonesia, originating from Prancak Village, Madura. Among the essential nutrients required for tobacco growth, nitrogen plays a crucial role in supporting vegetative and reproductive development. Although atmospheric nitrogen is abundant, constituting approximately 78% of the air, it is unavailable to plants in its diatomic form (N2). One sustainable approach to increasing nitrogen availability in soil is through the activity of non-symbiotic nitrogen-fixing bacteria (NFB), which convert atmospheric nitrogen into plant-accessible forms. This study aimed to isolate and identify non-symbiotic NFB from Prancak 95 tobacco farmland soils, in order to assess their population density, nitrogen-fixing potential, and taxonomic affiliation. Soil samples were collected from two locations in Prancak Village: West Tegal and Bilamabuk hamlets. Bacterial isolation was carried out using the pour plate method on Yeast Extract Mannitol Agar (YEMA) medium, followed by incubation at 37°C for 3 days. Pure bacterial isolates were subjected to macroscopic, microscopic, and biochemical characterisation based on Bergey's Manual of Determinative Bacteriology. Nitrogen-fixation potential was assessed by measuring the clear zone diameter on YEMA medium supplemented with Congo red, and the clear zone ratio was used as an indicator of nitrogenase activity. The results showed that the average non-symbiotic NFB population in West Tegal was 3.25 × 105 CFU.g-1, while in Bilamabuk it was 2.7 × 105 CFU.g-1. A total of six bacterial isolates were selected for further study. All isolates demonstrated nitrogen-fixing potential, with clear zone ratios ranging from 1.26 to 2.60 cm. Based on morphological, microscopic, and biochemical analyses, isolates coded PT2, PB1, and PB3 were identified as belonging to the genus Bacillus, while isolates PT1, PT3, and PB2 were tentatively identified as members of the genus Corynebacterium.
Co-Authors A. Arifiyanto Ach. Kholish Bayuda Luqman Al Farisi Cahyo Pitoyo, Alief Evatus Shafia fitria ningsih Irawan Deny Ananta junaidi, imroatussilviyah K. Khotim Kamilia, Lia M. A. Prio Mahrus Ali N. D. Kuswitasari rofiqoh rofiqoh Safitri, Soviana Fila Shovitri, Maya Vita Kurnia Utami