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Potensi Isolat Bakteri Edwardsiella dan Corynebacterium dari Pulau Poteran Sumenep sebagai Pelarut Fosfat Hefdiyah Hefdiyah; Maya Shovitri
Jurnal Sains dan Seni ITS Vol 3, No 2 (2014)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.392 KB) | DOI: 10.12962/j23373520.v3i2.6910

Abstract

Salah satu potensi Pulau Poteran Sumenep adalah bidang pertanian. Beberapa desa di Kecamatan Talango tanaman holtikultura ditanam di lahan kering dan kurang subur. Salah satu penyebabnya adalah rendahnya P-tersedia padahal dilaporkan P total tanah Madura adalah sedang hingga tinggi. Penelitian ini bertujuan mengisolasi Bakteri Pelarut Fosfat (BPF) tanah di Desa Talango dan Poteran agar diketahui keanekaragaman dan efisiensi pelarut fosfat bakteri tersebut. Isolasi bakteri dilakukan dengan metode pengenceran bertingkat dan metode sebar pada medium NA. Isolat murni diamati secara deskriptif makroskopis (bentuk, tepi, elevasi, dan warna koloni), mikroskopis (morfologi sel dan pewarnaan) dan karakter biokimia berdasarkan panduan Bergey’s Manual of Determinative serta diukur efisiensi pelarut fosfat dengan medium Pikovskaya. Berdasarkan hasil isolasi dan karakterisasi didapatkan 2 isolat berbeda yang kecenderungan masuk ke dalam dua genus Edwardsiella dan Corynebacterium. Efisiensi pelarut fosfat kedua isolat bakteri tersebut berturut-turut sebesar 43.86 dan 45.63 sehingga diasumsikan bakteri-bakteri tersebut berpotensi sebagai biofertilizer.
UJI POTENSI MEDIUM TUMBUH BERBAHAN DASAR EKSTRAK TAUGE, EKSTRAK BEKATUL, DAN EKSTRAK KULIT PISANG PADA KULTIVASI Spirullina spp. Hefdiyah Hefdiyah; A. Arifiyanto; K. Khotim; M. A. Prio; N. D. Kuswitasari
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 1 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Spirullina  spp. merupakan tumbuhan air yang berukuran mikroskopik, memiliki berbagai potensi yang dapat dikembangkan sebagai sumber pakan, pangan, industri  farmasi, dan makanan suplemen dengan kandungan protein, karbohidrat, lipid, dan berbagai macam mineral serta digunakan dalam pengolahan limbah logam berat sebagai pengikat logam dan dimanfaatkan sebagai sumber energi alternatif untuk biodiesel. Penelitian ini dilakukan untuk mengetahui potensi ekstrak tauge, bekatul dan ekstrak kulit pisang sebagai medium tumbuh pada kultivasi Spirullina spp. Pada penelitian ini menggunakan air laut sebagai medium kontrol (-)  dan Zarrouk sebagai medium kontrol (+) serta ektrak tauge, bekatul dan ekstrak kulit pisang masing-masing dengan konsentrasi 15% (perlakuan 1 : 150 ml ekstrak + 750 ml air laut + 100 ml spiru), 30% (perlakuan 2 : 300 ml ekstrak + 600 ml air laut + 100 ml spiru) dan 45% (perlakuan 3 : 450 ml ekstrak + 450 ml air laut + 100 ml spiru) dengan 3 kali ulangan. Dari hasil screening, Spirullina spp. tumbuh baik pada medium medium kontrol (-) dan kontrol (+) dan bekatul pada semua ulangan dengan laju peningkatan densitas 0.16, 0.057, 0.314, 0.261, dan 0.106. Hasil uji kandungan Spirullina spp dari 1L kultur Bekatul Perlakuan 1 (PB1) diperoleh berat kering 49.40663% dengan protein sebesar 19.9682%, lemak 4.895633% dan karbohidrat 24.5428%. PB2 diperoleh berat kering 29.75523% dengan protein sebesar 12.70963%, lemak 5.053867% dan karbohidrat 11.99173%. PB3 diperoleh berat kering 55.79287% dengan  protein sebesar 42.09023%, lemak 6.271949% dan karbohidrat 7.430684%. Perlakuan kontrol (-) diperoleh berat kering 32.9367% dengan protein sebesar 3.1159%, lemak 3.110133% dan karbohidrat 26.71067%. Dan perlakuan  kontrol (+) diperoleh berat kering 76.06407% dengan protein sebesar 40.66107%, lemak 9.274067% dan karbohidrat 26.12893%.  Kata kunci : Bekatul, Kulit Pisang, Kultivasi, Spirullina Spp, Tauge
Pertumbuhan Pin Head Jamur Kuping Hitam (Auricularia Polytricha) Pada Media Tambahan Tetes Tebu Dengan Dosis Berbeda Mahrus Ali; Hefdiyah Hefdiyah
EVOLUSI: JOURNAL OF MATHEMATICS AND SCIENCES Vol 5 No 2 (2021): Oktober 2021
Publisher : Fakultas MIPA Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/evolusi.v5i2.773

Abstract

Mushroom growing media is the main source of mushroom production, so it needs attention, for example by changing the composition of the growing media such as adding supporting nutrients. Sugarcane drops are waste from the process of making sugarcane crystallization into sugar which is thought to have an effect on fungal growth. The purpose of this study was to determine the effect of molasses and the best dose on the growth of pin head A. polytricha. This research was carried out with the addition of molasses with different doses, namely, P0 (control), P1 (20 ml/liter), P2 (40 ml/liter), P3 (60 ml/liter) and P4 (80 ml/liter). This study uses One Way Anova analysis and 5% BNJ. Based on the results of the study, it was known that the addition of nutrients to the growing media of A. polytricha in the form of molasses had a fairly effective effect on the growth of the pin head of A. polytricha, namely at an additional dose of 80 ml/liter.
Isolation and Identification of Non-Symbiotic Nitrogen-Fixing Bacteria in Prancak Village Tobacco Farming Soil Hefdiyah; Rofiqoh
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.04

Abstract

Prancak 95 is one of the most prominent tobacco (Nicotiana tabacum L.) varieties in Indonesia, originating from Prancak Village, Madura. Among the essential nutrients required for tobacco growth, nitrogen plays a crucial role in supporting vegetative and reproductive development. Although atmospheric nitrogen is abundant, constituting approximately 78% of the air, it is unavailable to plants in its diatomic form (N2). One sustainable approach to increasing nitrogen availability in soil is through the activity of non-symbiotic nitrogen-fixing bacteria (NFB), which convert atmospheric nitrogen into plant-accessible forms. This study aimed to isolate and identify non-symbiotic NFB from Prancak 95 tobacco farmland soils, in order to assess their population density, nitrogen-fixing potential, and taxonomic affiliation. Soil samples were collected from two locations in Prancak Village: West Tegal and Bilamabuk hamlets. Bacterial isolation was carried out using the pour plate method on Yeast Extract Mannitol Agar (YEMA) medium, followed by incubation at 37°C for 3 days. Pure bacterial isolates were subjected to macroscopic, microscopic, and biochemical characterisation based on Bergey's Manual of Determinative Bacteriology. Nitrogen-fixation potential was assessed by measuring the clear zone diameter on YEMA medium supplemented with Congo red, and the clear zone ratio was used as an indicator of nitrogenase activity. The results showed that the average non-symbiotic NFB population in West Tegal was 3.25 × 105 CFU.g-1, while in Bilamabuk it was 2.7 × 105 CFU.g-1. A total of six bacterial isolates were selected for further study. All isolates demonstrated nitrogen-fixing potential, with clear zone ratios ranging from 1.26 to 2.60 cm. Based on morphological, microscopic, and biochemical analyses, isolates coded PT2, PB1, and PB3 were identified as belonging to the genus Bacillus, while isolates PT1, PT3, and PB2 were tentatively identified as members of the genus Corynebacterium.
ISOLASI, ENUMERASI, DAN KARAKTERISASI BAKTERI PELARUT FOSFAT (BPF) INDIGENUS TANAH DESA KEBUNAN SUMENEP SEBAGAI AGEN PUPUK HAYATI ORGANIK Hefdiyah, H.; Ali, Mahrus; Utami, Vita Kurnia
Jurnal Biosilampari : Jurnal Biologi Vol 5 No 2 (2023): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v5i2.1972

Abstract

Phosphate Solubilizing Bacteria (PSB) are known that have important role to improve plant phosphate uptake as biofertilizer. These Microorganism hold potential to promote organic agriculture as eco friendly and efficiency. This study aims to isolate, enumerate and characterize indigenous PSB from Kebunan Village soil as the organic biofertilizer agent. Isolation and enumeration were carried out using the spread plate method on PCA media for enumeration of the total bacteria and Pikovskaya media (PKV) and NBRIP for BPF selection and enumeration. characterization includes observing the morphological colonies and cells. These experimental studies found that the soil pH was 6.5, the temperature range was 28-35 °C, the total P content was from 0.59 to 1.09 and the dissolved phosphate content sequentially from the smallest is 0.53 (KB2), 29.41 (KB1), 60.45 (KB3). The total abundance of bacteria at 3 different locations showed variations between 1.97 x 106 to 287 x 106. The highest abundance of BPF reached 5.45 x 106 cfu/g was in the watershed which had the lowest available P content. Total 7 isolates of BPF were successfully isolated and characterized with different colony characters and cell shapes. The selected BPF isolates were dominated by Gram-positive bacilli.
Perbandingan Penggunaan Media Cair Ekstrak Kedelai (Glycine max), Kacang Tanah (Arachis hypogaea L.), dan Kacang Hijau (Vigna radiata L) dalam Perbanyakan Pseudomonas fluorescens sebagai Agen Hayati Ningsih, Fitria; Hefdiyah, Hefdiyah; Cahyo Pitoyo, Alief
Bioconsortium : Biological Research and Education Vol 1 No 1 (2024): Bioconsortium : Biological Research and Education
Publisher : Program Studi Biologi Fakultas Matematika dan IPA Universitas Annuqayah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59005/bioconsortium.v1i1.301

Abstract

Pseudomonas fluorescens is one of the antagonist bacteria because it has the ability to induce systemic resistance. P. fluorescens has also been reported to increase the content of phenolic compounds in plants. These bacterium are widely used as biological agents for fungi, pathogenic bacteria and viruses in plants. The research method used in this research is the TPC method. The average number of P. fluorescens bacterial colonies obtained on each of the soybean, peanut and mung bean extract media was 1,68 x 108 CFU/ml, 4,44 x 108 CFU/ml and 6,08 x 108 CFU/ml. The purpose of this study was to determine which media was more effective against P. florescens bacteria in its growth on liquid extracts of soybean, peanut, and mung bean. The results indicate that the most effective media for the growth of P. fluorescens bacteria was the liquid media of mung bean extract, with an average total colony count of 6,08 x 108 CFU/ml.
Chemical Examination of Methanyl Yellow in Processed Food Products at the Blumbungan Market Pamekasan District Kamilia, Lia; Hefdiyah
Bioconsortium : Biological Research and Education Vol 1 No 2 (2024): Bioconsortium : Biological Research and Education
Publisher : Program Studi Biologi Fakultas Matematika dan IPA Universitas Annuqayah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59005/bioconsortium.v1i2.527

Abstract

Non-food grade dyes such as textile dyes are widely used by rogue manufacturers to give color to their food products. The example is textile dyes methanyl yellow. Methanyl yellow is made from methanylic acid and diphenylamine. Both of these ingredients are toxic. This research is used the metanyl yellow rapid test kit method by taking several types of food samples suspected of containing metanyl yellow. From the three samples such as chicken skin, rambak crackers and color crackers, there are two samples positive for methanyl yellow, chicken skin and color crackers. This is expressed by a change in the color of the yellow reagent to purple. Based on the research, it can be concluded that there are 2 types of samples containing methanyl yellow dye at the Blumbungan market in Pemekasan Regency.
DIET SAMPAH BERSAMA PENYULUHAN PENTINGNYA MENJAGA LINGKUNGAN SEKITAR DANMPEMBUATAN KERAJINAN DARI BOTOL BEKAS junaidi, imroatussilviyah; hefdiyah
ABDISUCI : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 1 No 3 (2023): ABDISUCI : Jurnal Pengabdian dan Pemberdayaan Masyarakat Oktober 2023
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59005/j-abdisuci.v1i3.127

Abstract

Pemeliharaan kebersihan di Desa Rombasan masih terbilang kurang optimal terutama dalam pengelolaan sampah karena lingkungan yang bersih dan terpelihara dengan baik akan berdampak baik terhadap kesehatan masyarakat di Desa Rombasan, perlu adanya upaya untuk meningkatkan kebersihan lingkungan tersebut, oleh karena itu dilaksanakan penyuluhan tentang kebersihan lingkungan dan bahaya sampah dan menyediakan tempat sampah yang terbuat dari botol-botol bekas minuman yang dilengkapi dengan label organik dan anorganik. Kegiatan ini bertujuan agar masyarakat Rombasan sadar akan pentingnya pola hidup sehat dan menjaga kebersihan lingkungan, salah satunya dengan membuang sampah pada tempatnya, metode pelaksanaan kegiatan pengabdian kepada masyarakat ini adalah dengan penyuluhan sistem diskusi yang bertempat di balai Desa Rombasan dengan peserta ibu-ibu rumah tangga, penyuluhan kesehatan lingkungan tentang kebersihan lingkungan dan bahaya sampah menjadi tema dalam pengabdian masyarakat ini. Hasil dari pengabdian kepada masyarakat ini menunjukan masyarakat antusias mengikuti kegiatan, selain itu masyarakat juga bertambah pengetahuannya tentang definisi bersih, ciri–ciri bersih, jenis–jenis kebersihan, manfaat kebersihan, aspek yang dibutuhkan mewujudkan kebersihan serta akibat yang ditimbulkan apabila tidak menjaga kebersihan dan dampak bagi kehidupan yang berkeberlanjutan ini.Kata Kunci: Desa Rombasan. Lingkungan, Sampah.