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All Journal Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Biosaintifika: Journal of Biology & Biology Education Jurnal Biosilampari: Jurnal Biologi Evolusi: Journal of Mathematics and Natural Sciences Genbinesia Journal of Biology
Mahrus Ali
Insitut Sains dan Tenologi Annuqayah
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Mathematics

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DNA Barcoding: A Study of Guppy Fish (Poecilia reticulata) in East Java, Indonesia Hariyanto, Sucipto; Adro’i, Hasan; Ali, Mahrus; Irawan, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (870.898 KB) | DOI: 10.15294/biosaintifika.v11i2.20222

Abstract

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.
Pertumbuhan Pin Head Jamur Kuping Hitam (Auricularia Polytricha) Pada Media Tambahan Tetes Tebu Dengan Dosis Berbeda Mahrus Ali; Hefdiyah Hefdiyah
EVOLUSI: JOURNAL OF MATHEMATICS AND SCIENCES Vol 5 No 2 (2021): Oktober 2021
Publisher : Fakultas MIPA Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/evolusi.v5i2.773

Abstract

Mushroom growing media is the main source of mushroom production, so it needs attention, for example by changing the composition of the growing media such as adding supporting nutrients. Sugarcane drops are waste from the process of making sugarcane crystallization into sugar which is thought to have an effect on fungal growth. The purpose of this study was to determine the effect of molasses and the best dose on the growth of pin head A. polytricha. This research was carried out with the addition of molasses with different doses, namely, P0 (control), P1 (20 ml/liter), P2 (40 ml/liter), P3 (60 ml/liter) and P4 (80 ml/liter). This study uses One Way Anova analysis and 5% BNJ. Based on the results of the study, it was known that the addition of nutrients to the growing media of A. polytricha in the form of molasses had a fairly effective effect on the growth of the pin head of A. polytricha, namely at an additional dose of 80 ml/liter.
ISOLASI, ENUMERASI, DAN KARAKTERISASI BAKTERI PELARUT FOSFAT (BPF) INDIGENUS TANAH DESA KEBUNAN SUMENEP SEBAGAI AGEN PUPUK HAYATI ORGANIK Hefdiyah, H.; Ali, Mahrus; Utami, Vita Kurnia
Jurnal Biosilampari : Jurnal Biologi Vol 5 No 2 (2023): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v5i2.1972

Abstract

Phosphate Solubilizing Bacteria (PSB) are known that have important role to improve plant phosphate uptake as biofertilizer. These Microorganism hold potential to promote organic agriculture as eco friendly and efficiency. This study aims to isolate, enumerate and characterize indigenous PSB from Kebunan Village soil as the organic biofertilizer agent. Isolation and enumeration were carried out using the spread plate method on PCA media for enumeration of the total bacteria and Pikovskaya media (PKV) and NBRIP for BPF selection and enumeration. characterization includes observing the morphological colonies and cells. These experimental studies found that the soil pH was 6.5, the temperature range was 28-35 °C, the total P content was from 0.59 to 1.09 and the dissolved phosphate content sequentially from the smallest is 0.53 (KB2), 29.41 (KB1), 60.45 (KB3). The total abundance of bacteria at 3 different locations showed variations between 1.97 x 106 to 287 x 106. The highest abundance of BPF reached 5.45 x 106 cfu/g was in the watershed which had the lowest available P content. Total 7 isolates of BPF were successfully isolated and characterized with different colony characters and cell shapes. The selected BPF isolates were dominated by Gram-positive bacilli.
Analysis of diversity of macrobenthos in mangrove ecosystems as a bioindicator of pollution of the Kalianget coastal area Ferdiansyah, Akhmad; Ali, Mahrus
Genbinesia Journal of Biology Vol. 1 No. 2 (2022): March 2022
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v1i2.10

Abstract

Macrobenthos is a biological resource of water that lives on the bottom of the waters with characteristics that have a high level of sensitivity to changes in environmental quality as a bioindicator of pollution and environmental quality. The purpose of the study was to determine macrobenthos diversity as an indicator of pollution in the Kalianget coastal mangrove forest. Descriptive research with random sampling method with squared transects. Data collection through observation of macrobenthos populations. Research results a) the greatest diversity of macrobenthos of the class Crustaceans obtained 9 species, class Gastropods 5 species, class Bivalves found 3 species and from class Polychaeta found 1 species. b) The Macrobenthos Diversity Index on the Kalianget coast is 1.084539 with the category of moderate macrobenthos diversity, has moderate community stability and polluted waters but is still within the threshold that living things can tolerate.Diversity; Index diversity; Macrobenthos; Mangrove ecosystems
Keanekaragaman dan Kelimpahan Vegetasi Riparian di Sungai Saroka Kecamatan Saronggi Kabupaten Sumenep Ali, Mahrus; Wulandari, Sri Riani; Ferdiansyah, Akhmad
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.563

Abstract

Riparian vegetation is a plant community of several types of habitus such as trees, saplings and seedlings that live on river banks. The characteristics of riparian vegetation which have specifically adapted to environmental conditions make it have many strategic functions in maintaining the ecosystem. Some of the positive impacts of riparian vegetation depend on the varying structure and composition of the vegetation. The destruction of riparian vegetation will result in a decrease in the function of riparian vegetation in maintaining the ecosystem. The aim of this research is to study the diversity and abundance of riparian vegetation and to study environmental factors that influence the diversity and abundance of riparian vegetation. The method used is the belt transect method. The method is to make 4 belt transects, each measuring 10m x 80m. Each belt transect is made into a quadrant measuring 10m x 10m which is installed perpendicular to the river bank. Each quadrant is placed with a sampling plot measuring 2m x 2m for seedlings, 5m x 5m for saplings, and 10m x 10m for trees. Vegetation analysis includes: importance value index, diversity index (H'), uniformity index (E), and dominance index (C). The research was conducted on the Saroka River, Saronggi District, Sumenep Regency. The results of this research were the discovery of 37 types of riparian vegetation from 20 families whose habit was trees, saplings and seedlings. The highest INP for tree habitus is dominated by banana species, sapling habitus is dominated by cassava, while INP for seedling habitus is puzzle. The diversity index at all research stations is moderate (H'= 2.12), the uniformity index is high (E= 0.75) with a dominance index of 0.17 (almost no individuals dominate). Environmental factors that influence the diversity and abundance of riparian vegetation are air humidity, soil pH and environmental temperature.
Eksplorasi Etnobotani Sebagai Obat Tradisional Masyarakat Desa Duko Kecamatan Rubaru - Sumenep Argus, Argus; Zahiroh, Syarifahtuz; Ali, Mahrus
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.564

Abstract

The utilization of plants as traditional medicine has been known for a long time by the people of Duko Village, Rubaru Subdistrict, Sumenep District. However, there is no data that can be used as an archive about plants in Duko Village, Rubaru District, Sumenep Regency. This study aims to determine the exploration of ethnobotanical diversity as traditional medicine and its parts used in traditional medicine and how it is utilized. This research is a non-experimental qualitative descriptive research and literature study. This research used field survey methods, semi-structured interviews, and documentation. This research was conducted in March-May 2023 in Duko Village, Rubaru District, Sumenep Regency. Sampling was carried out using purposive sampling technique in three hamlets, namely East Pedatar Hamlet, West Pedatar Hamlet, and Laok Lorong Hamlet, so that 97 respondents were obtained. Data analysis in this study used the Use Value Species (Uvs) method. The results showed that there were 107 species used by the people of Duko Village as traditional medicine to treat various diseases. Plant parts used include leaves with a percentage of 51%, fruit 26%, rhizomes 14%, flowers 11%, seeds 8%, tubers 7%, roots 7%, sap 6%, stems 4%, bark 2%, and plant hair parts 1%. Ways of utilizing plants in medicine include boiling, extracting, mashing, consuming directly, and roasting
Co-Authors Adro’i, Hasan Akhmad Ferdiansyah, Akhmad Argus Argus, Argus Hefdiyah Hefdiyah Maemonah, Maemonah Sucipto Hariyanto Vita Kurnia Utami Wulandari, Sri Riani Zahiroh, Syarifahtuz