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Pengaruh Variasi Konsentrasi NaNO3 pada Medium Raoof terhadap Kultivasi Spirulina Platensis Iqbal Syaichurrozi; Wardalia Wardalia; Sharfan Dwicahyanto; Yustinus Selis Toron
Eksergi Vol 19, No 1 (2022)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v19i1.6581

Abstract

Nutrition is one of the factors that affect the growth of Spirulina platensis. The cultivation medium proposed by Raoof is a modified version of Zarrouk medium. Raoof medium requires less nutrients than Zarrouk medium but the biomass produced is almost the same. This study was conducted to vary the concentration of NaNO3 of 0.5; 1.5; 2.5; 3.5 g/L in Raoof medium to study its effect on growth and biomass composition of S. platensis. At each concentration of NaNO3 of 0.5; 1.5; 2.5; 3.5 g/L obtained the highest biomass concentration of 0.6425; 0.6455; 0.6745; 0,5193 g/L on days 12, 12, 12, 10. Concentration of NaNO3 of 2.5 g/L resulted in the highest specific growth rate of 0.1371/day and the lowest double time of 5.0566 days. In general, increasing the concentration of NaNO3 from 0.5 to 2.5 g/L increased the protein content from 33.30 to 36.98%. The addition of higher NaNO3 (3.5 g/L) actually decreased the protein content to 35.52%.
Pengaruh Perbedaan Jenis dan Konsentrasi Sumber Nitrogen (NaNO3 dan urea) terhadap Produksi Biomasa Spirulina Platensis Iqbal Syaichurrozi; Yustinus Selis Toron; Sharfan Dwicahyanto; Wardalia Wardalia
Eksergi Vol 20, No 2 (2023)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v20i2.9367

Abstract

The goal of this study was to investigate the effect of the concentration and the type of nitrogen sources in the cultivation medium on the production of biomass and biochemical content (carbohydrates, proteins, fats) in the Spirulina platensis biomass. Types of nitrogen sources included NaNO3 and urea, where the concentrations were varied from 0.5 to 3.5 g/L. The results showed that the use of urea tended to produce more S. platensis biomass than the use of NaNO3. The best concentrations that produced the highest biomass were NaNO3 2.5 g/L (biomass 0.6745 g/L) and urea 0.5 g/L (biomass 0.7158 g/L). The use of urea also tended to produce a higher specific growth rate and a shorter doubling time than the use of NaNO3. The high concentration of nitrogen sources caused the pH of the medium to increase more rapidly due to the accumulation of ammonium in the medium. Using NaNO3 can produce biomass with a higher protein content (28.34-36.98%) than using urea (25.86-33.52%).