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Endah Wahyurini
Universitas Pembangunan Nasional "Veteran" Yogyakarta
Agriculture, Biological Sciences & Forestry

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The Effect of 2,4 d on Multiplication Eksplan of Various Dragon Fruit (hylocereus sp) by In Vitro Endah Wahyurini; Susilowati Susilowati
Jurnal Agrivet Vol 23, No 2 (2017): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v23i2.4774

Abstract

Propagation of seedling by tissue culture technique is challenging step during cultivation of these plant. The addition of 2.4 D is very influential on multiplication plant on various varieties of dragon fruit. This research aimed to determine the interaction between varieties and 2.4 D for the multiplication of planlet, to determine the right varieties of plants and to determine the appropriate 2.4 D concentration yielding superior plantlet. The experiments were performed using Complete Random Design with two treatments and three replication. The first factor is varieties wich four ll evetreatments of red dragon fruit, super red, white and yellow. The second factor is 2,4 D which also consists of three level of treatment that is 2 mg / L, 3 mg / L and 4 mg / L. The results were analyzed using ANOVA with further Duncan Multiple Range Test at the level 5%. The results showed that the white of dragon fruit showed the longest shoot height, the use of 2.4 D did not affect the multiplication of dragon fruit eksplan, and the combination of white dragon fruit treatment with 2.4 D at 2 mg / L concentration gave a better influence in inducing growth of fresh weight of planlet, dry weight of planlet and antioxidant compound. While red dragon fruit with 2.4 D at concentration 3 mg / L give a better influence in inducing root length and root number.Keywords: 2,4 D, varieties of dragon fruit, in vitro.
THE GROWTH OF “PISANG RAJA BULU” PLANLETS IN THE LIGHTING OF INCUBATION ROOMS AND GROWTH REGULATOR AGENTS OF BROWNING PREVENTION BY IN VITRO Utami Setyawati; Ari Wijayani; Endah Wahyurini
Jurnal Agrivet Vol 25, No 1 (2019): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v25i1.4170

Abstract

The aims of this research were to determine the lighting of incubation rooms and browning prevention agents on the growth of “Pisang Raja Bulu” planlets by in vitro and determine whether there is a best interaction between the incubation rooms lighting and the type of browning prevention agents in the growth of “Pisang Raja Bulu” planlets that plants in vitro. This research was conducted in the Biotechnology laboratory of the Faculty of Agriculture, UPN "Veteran" Yogyakarta on June - August 2018. The research method was a laboratory experiment method compiled with the Split Plot Design of two factors. The first factor as a main plot is the incubation room lighting, which is with light for 90 days, without light for 90 days, without light in the first 45 days, and without light in the last 45 days. The second factor as a sub plot is browning prevention agents, named thidiazuron, activated charcoal, and vitamin C. Each combination of treatments was repeated 3 times. The result showed that there was the best combinations of treatments that is all combination of lighting and vitamin C 0,88 mg / l in terms of browning. The first 45 days lighting treatment gave the best result on the percentage of life, plantlet height, number of shoots, number of leaves, root length, and fresh weight. The treatment of vitamin C 0.88 mg / l gave the best result on the percentage of life, plantlet height, number of shoots, root length, number of roots, fresh weight, and dry weight.Keyword: in vitro, lighting, browning, raja bulu banana.
IN VITRO PLANLET INDUCTION OF TROPICAL PITCHER PLANT (NEPENTHES AMPULLARIA JACK) BY VARIOUS THIAMIN AND BENZYL AMINO PURINE CONCENTRATE Arwinda Dinar Cryssanti; Ari Wijayani; Endah Wahyurini
Jurnal Agrivet Vol 25, No 2 (2019): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v25i2.4285

Abstract

Nepenthes is one of Indonesian tropical plant as a biodiversity source that endangered from its extinction. One of the effort to prevent its extinction by using plant tissue isolation method on Nepenthes multiplication are needed. This research was aimed to determine the best interaction between Thiamin and benzyl amino purine concentration on in vitro Nepenthes planlet growth, Thiamin best concentration on in vitro Nepenthes planlet growth, and benzyl amino purine best concentration on in vitro Nepenthes planlet growth. The research was conducted in Agriculture Department Biotechnology Laboratory Universitas Pembangunan Nasional “Veteran” Yogyakarta on January – April 2018. Completed Random Design method with 2 factors was used in this research. The first factor was various concentration of Thiamin with 8 ppm, 10 ppm, and 12 ppm. The second factors was various concentration of benzyl amino purine with 0,5 ppm, 1 ppm, and 1,5 ppm. Every combination was repeated 3 times. The result of this research showed that no interaction was found in every Thiamin and benzyl amino purine various concentration on in vitro Nepenthes planlet. The usage of 10 ppm Thiamin concentration (T2) produce the best results on sprout’s amount and sprout’s height. On 1 ppm of benzyl amino purine concentration produce the best result on sprout’s amount, sptout’s height, and amount of leaves.Keyword: Nepenthes, Thiamin, Benzyl Amino Purine
IN VITRO SHOOT INDUCTION OF GARUT (Maranta arundinacea) WITH THE ADDITION OF 2,4-D BENZYL ADENIN Endah Wahyurini
Jurnal Agrivet Vol 26, No 1 (2020): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v26i1.4314

Abstract

Arrowroot (Maranta arundinacea L.) is a type of forest tuber plant that potentially developed as a local food crop. Arrowroot tubers can be processed as chips and starches. The difficulty of getting superior varieties and seeds in relatively large quantities and uniform can be overcome by in vitro techniques. The success of tissue culture depends on the planting media, PGR, vitamins and plant genetics. The purpose of this study was to determine the effect of giving 2.4 D and BA on the growth of arrowroot explants, as well as getting the proper concentrations of 2.4 D and BA to stimulate the growth of arrowroot tubers in vitro. The study wa conducted in a laboratory with two factors Complete Random Design Method. The first factor is the 2.4 D concentration which consists of three levels, namely: 0.5 mg / L (D1), 1 mg / L (D2) and 1.5 mg / L (D3). The second factor is the concentration of BA consisting of three levels, namely: 1 mg / L (B1), 2 mg / L (B2) and 3 mg / L (B3). Variance analysis were done at 5% level. To find out there is a real difference between treatments, Duncan Multiple Range Test (DMRT) at 5% level were conducted. The results showed that the 2.4 D treatment concentration of 1 mg / L produced a greater percentage of shoot life than other treatments. Giving 2.4 D concentrations of 1 mg / L and BA 2 mg / L stimulated quicker emergence of shoots and higher shoot lengths compared to other treatments.Keyword: 2,4 D, Benzyl adenine, Maranta arundinacea, in vitro
Co-Authors Ari Wijayani Arwinda Dinar Cryssanti Susilowati Susilowati Utami Setyawati