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COMBINATION OF BROWNING INHIBITOR SUBSTANCE AND SUCROSE ON THE GROWTH OF MAS KIRANA BANANA PLANLET(Musa acuminata C.) IN VITRO Dofan Rizki Baskara; Ari Wijayani; Rina Srilestari
Jurnal Agrivet Vol 24, No 1 (2018): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v24i1.4662

Abstract

Mas Kirana Banana (Musa acuminata C.) is one of the most popular tropical fruit plants in the community. The problem of banana tissue culture is browning on the media against phenolic substances that need to be done before the browning inhibitor. This study was conducted to determine the effect of browning inhibitor substance and sucrose on the growth of plantlet banana mas kirana. Research method with RAL (Completely Random Design) two factors + 1 control. The first ingredient consisted of 3 levels, namely polyvinylpyrrolidone 75 g / L, Vitamin C 0,50 ppm, Active Charcoal 1 g / L, while the second factor consisted of 3 levels, sucrose 15 g / L, 20 g / L and 25 g / L Each treatment was repeated 3 times. Data with anova and test with DMRT at 5% level. Browning inhibitors Vitamin C results in the number of shoots, wet weight,  dry weight  and  browning level compared with polyvinylpyrrolidone and activated charcoal. The addition of sucrose concentrations of 20 g / L canincrease length of rootKeywords: Banana, tissue culture, browning inhibitor substance, sucrose 
Induction of “Sakuntala” chrysanthemum bud using in-vitro culture Tutut Wirawati; Ari Wijayani; Rina Srilestari
Jurnal Agrivet Vol 18, No 1 (2012): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v18i1.4645

Abstract

The research was to determine the best combination of Chrysanthemum explant and concentrations of BAP (6 - benzyl amino purine), the effect of the explant material and the effect concentrations of BAP (6 - benzyl amino purine) in vitro culture by Murashige and Skoog medium. The experimental methode was Completely Randomizes Design (CRD) which consisted of two factors. The first factor is the explant material, consisting of three standards are: P1 = node, P2 = shoots, P3 = leaf. The second factor is the concentration of BAP which consists of four levels, namely: B1 = 0.5 ppm, 1.0 ppm = B2, B3 = 1.5 ppm, B4 = 2.0 ppm. The results showed there were significant interaction on fresh weight, dry weight, number of shoots and length of shoots. The treatment combination between bud explants with BAP 0.5 ppm, showed the best results on fresh weight, number of shoots and long shoots. Use of explant shoots, can accelerate the current growth of shoots, expanding the number and length of root buds. The addition of plant growth regulators BAP 0.5 ppm in the tissue culture medium, can spur the rapid growth of shoots, root number and length of root best.Key words : in vitro, chrysanthemum, kinds of eksplant, the concentration of BAP
Addition Of Gibberellin (GA3) to Gladiolus Cormel Growth (Gladiolus communis L.) on Various Medium in Vitro Egy Aerani; Rina Srilestari; Ari Wijayani
Jurnal Agrivet Vol 23, No 2 (2017): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v23i2.4811

Abstract

Gladiolus flower can be multiplied by using corm or cormel. The multiplication of this flower using plating material takes a long time, about 3,5-6 months. By using in vitro method, the seed of this flower can be produce in relatively short time. The aim of this research was to determine the interaction of gibberellins on any kind of media (MS, B5, and MS + vitamin B5), to determine the perfect combination of concentration of Gibberelin, and to determine the perfect type of media that can be use for induction of root and shoot of gladiolus cormel. The research design used was a Completely Randomized Design (CRD) of the factorial pattern with 3 replications. The first factors (concentration of Gibberellin) G1: 1 ppm, G2: 2 ppm, dan G3: 3 ppm, the second factors (type of media) M1: MS , M2: B5 (Gamborg) , M3: MS + B5 vitamin. The result showed that the addition of various concentration of Gibberelin (GA3) and any type of media had no significantly improve the growth of gladiol cormel explant, by addition concentration of Gibberelin 2 ppm has significantly improved fresh weight of planlet and percentage of life, by addition B5 media, has significantly improved length of root and growth of shoot, and by using MS media with B5 vitamin has significantly improved the growth of shoot, height and fresh weight of planlet.Keywords: Gladiol, gibberellin, plant media, in vitro
THE GROWTH OF “PISANG RAJA BULU” PLANLETS IN THE LIGHTING OF INCUBATION ROOMS AND GROWTH REGULATOR AGENTS OF BROWNING PREVENTION BY IN VITRO Utami Setyawati; Ari Wijayani; Endah Wahyurini
Jurnal Agrivet Vol 25, No 1 (2019): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v25i1.4170

Abstract

The aims of this research were to determine the lighting of incubation rooms and browning prevention agents on the growth of “Pisang Raja Bulu” planlets by in vitro and determine whether there is a best interaction between the incubation rooms lighting and the type of browning prevention agents in the growth of “Pisang Raja Bulu” planlets that plants in vitro. This research was conducted in the Biotechnology laboratory of the Faculty of Agriculture, UPN "Veteran" Yogyakarta on June - August 2018. The research method was a laboratory experiment method compiled with the Split Plot Design of two factors. The first factor as a main plot is the incubation room lighting, which is with light for 90 days, without light for 90 days, without light in the first 45 days, and without light in the last 45 days. The second factor as a sub plot is browning prevention agents, named thidiazuron, activated charcoal, and vitamin C. Each combination of treatments was repeated 3 times. The result showed that there was the best combinations of treatments that is all combination of lighting and vitamin C 0,88 mg / l in terms of browning. The first 45 days lighting treatment gave the best result on the percentage of life, plantlet height, number of shoots, number of leaves, root length, and fresh weight. The treatment of vitamin C 0.88 mg / l gave the best result on the percentage of life, plantlet height, number of shoots, root length, number of roots, fresh weight, and dry weight.Keyword: in vitro, lighting, browning, raja bulu banana.
Growth and Brix of Sweet Sorghum under Different Fertilizer Application in Maginal Land Rukmowati Brotodjojo; Mohammad Nurcholis; T Marnoto; Ari Wijayani; Rochman Isdiyanto
Jurnal Agrivet Vol 23, No 2 (2017): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v23i2.4738

Abstract

Sorghum is one of the commodities that are potentially very good to meet the needs of food, feed, industrial and renewable energy sources. Sorghum has protein, iron and calcium content that is much higher than rice. Additionally, sorghum has wide adaptability and is a plant that is tolerant to drought and low fertility. Therefore it can be cultivated on marginal lands. This study aimed to study the effects of fertilization on the growth and brix value of sweet sorghum cultivated on marginal land. The experiment was arranged in randomized completely block design, with three treatments, namely NPK, NPK + LOF (Liquid Organic Fertilizer) / 1 week, NPK + LOF/ 2 weeks. Each treatment consisted of 5 replication, and for each replication 6 plant samples was observed. Each plot consisted of 12 rows and in each row there were 7 plants. Sorghum was planted with the distance between rows 75 cm and the distance between plants in rows was 30 cm. The data was subjected to analysis of variance followed by Duncan’s Multiple Range Test. The application of NPK fertilizer plus LOF once a week resulted in significantly higher plant height, number of leaves and brix value compared to NPK fertilizer plus LOF fortnightly or just NPK fertilizer alone. Fertilization treatment did not significantly affect sorghum stem diameter, except at the age of 3 weeks after planting.Keywords: fertilization, growth, brix, sweet sorghum.
IN VITRO PLANLET INDUCTION OF TROPICAL PITCHER PLANT (NEPENTHES AMPULLARIA JACK) BY VARIOUS THIAMIN AND BENZYL AMINO PURINE CONCENTRATE Arwinda Dinar Cryssanti; Ari Wijayani; Endah Wahyurini
Jurnal Agrivet Vol 25, No 2 (2019): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v25i2.4285

Abstract

Nepenthes is one of Indonesian tropical plant as a biodiversity source that endangered from its extinction. One of the effort to prevent its extinction by using plant tissue isolation method on Nepenthes multiplication are needed. This research was aimed to determine the best interaction between Thiamin and benzyl amino purine concentration on in vitro Nepenthes planlet growth, Thiamin best concentration on in vitro Nepenthes planlet growth, and benzyl amino purine best concentration on in vitro Nepenthes planlet growth. The research was conducted in Agriculture Department Biotechnology Laboratory Universitas Pembangunan Nasional “Veteran” Yogyakarta on January – April 2018. Completed Random Design method with 2 factors was used in this research. The first factor was various concentration of Thiamin with 8 ppm, 10 ppm, and 12 ppm. The second factors was various concentration of benzyl amino purine with 0,5 ppm, 1 ppm, and 1,5 ppm. Every combination was repeated 3 times. The result of this research showed that no interaction was found in every Thiamin and benzyl amino purine various concentration on in vitro Nepenthes planlet. The usage of 10 ppm Thiamin concentration (T2) produce the best results on sprout’s amount and sprout’s height. On 1 ppm of benzyl amino purine concentration produce the best result on sprout’s amount, sptout’s height, and amount of leaves.Keyword: Nepenthes, Thiamin, Benzyl Amino Purine
ARABICA AND ROBUSTA COFFEE PRODUCTION IN DIFFERENT GROWING ENVIRONMENTS ON THE SLOPES of MOUNT ARJUNA MALANG Ari Wijayani; Sari Virgawati; Ninik Probosari
Jurnal Agrivet Vol 27, No 2 (2021): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v27i2.5654

Abstract

The area on the slopes of Mount Arjuna that has the potential to be developed as a center for Arjuna coffee is Toyomarto, which is located in Singosari District, Malang Regency at an altitude of 700-1400 meters above sea level. The types of coffee produced from the Toyomarto, Singosari plantations are Arabica and Robusta. The characteristics of the coffee have a sweet-sour character and a fruity aroma such as bananas, apples, and so on. The problem, in the field, shows that the low quality of coffee is due to the absence of standard methods of cultivation and post-harvest handling. At different altitudes, it shows differences in coffee production as well. The average productivity of Arabica coffee at an altitude of 1100 m above sea level is 55.97 tons, significantly different from coffee cultivated at an altitude below 1000 m above sea level. Meanwhile, Robusta coffee production is not significantly different at all altitudes where it is cultivated.
The improvement of agronomic traits of chrysanthemum through additional lighting and flower faselife assessment in Hargobinangun, Sleman, DIY Ari Wijayani; Tutut Wirawati; Wongsoyudo Wongsoyudo
Jurnal Agrivet Vol 18, No 2 (2012): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v18i2.4650

Abstract

Chrysanthemum cultivation in medium height plains is still facing obstacles where the agronomic properties are not as good as in the highlands. It is important to find cultivation techniques that can improve agronomic properties of chrysanthemum. The setting of additional lighting technique remains to be learned then applied at planting chrysanthemum plain medium. The research purpose is to determine the best additional length of radiation for the improvement of agronomic properties of chrysanthemum. In addition to determining the best chrysanthemum varieties to be developed in the medium height plain as Hargobinangun. The research has been conducted in Hargobinangun, Sleman in April-August 2011 in two stages. The first stage is to determine the length of the addition of irradiation to improve the agronomic properties of chrysanthemum, namely 2,3,4 and 5 hours in the evening starting at 22:00 pm. While the second stage is to look at various kinds of flower faselife varieties, namely Sakuntala, Snow white, Wastu kania, Shamrock, Puspita Nusantara, Padmabuana and Tirta ayuni. The results was showing that cultivation techniques with the addition of irradiation for 5 hours will improve agronomic traits such as chrysanthemum plant height, stem diameter, flower diameter, flower number and brightness of flower color ribbon. While the flower varieties that have good prospects to be developed in the plain medium Hargobinangun because it has a much longer period of flower freshness is Puspita Nusantara, Sakuntala and Snow White.Key words: chrysanthemum, additional lighting, faselife of flowers
CALLUS REGENERATION OF Chrysanthemum AFTER GAMMA RAY IRRADIATION FOR THE RESILIENCE OF MEDIUM PLAIN Ari Wijayani; Muafi Muafi; Endah Wahyurini; Rina Sri Lestari
Techno LPPM Vol 1, No 1 (2015)
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Development of chrysanthemum plants in the medium plains is still facing obstacles due tounfavorable climate. Until today, the availability of tolerant chrysanthemum seeds grown in the mediumplains is not maximized so that the necessary effort in order to increase the genetic diversity as thematerial selection to obtain tolerant chrysanthemums grown in medium plain. This research is beingconducted in order to follow up the problems of availability of tolerant chrysanthemums seeds grown inthe medium plains at several stages. Stage one is the induction of callus after gamma ray irradiation invitro. This research has been conducted in tissue culture laboratory of the Faculty of Agriculture UPN"Veteran" Yogyakarta from February to June 2015. Tested regeneration media is ½ MS with the additionof IAA 0.1 mg/l; 0.2 mg/l; 0.3 mg/l; 0.4 mg/l and 0.5 mg/l. The results showed that ½MS regenerationmedia with the addition of IAA 0.3 mg/l spur sprouts emerge percentage (100%); while growing sprouts(8.67 days), height are about sprouts (3:10 cm) and number of sprout (7:00). Regeneration mediumTreatment with kinetin 2 mg/l + IAA 0.5 mg/l gives the number of root growth (13) and root length (4:07cm)Keywords: Chrysanthemum, tolerant medium plains, in vitro selection, gamma ray irradiation,
Usaha Meningkatkan Kualitas Beberapa Varietas Tomat dengan sistem Hidroponik Ari Wijayani; Wahyu Widodo
Jurnal Ilmu Pertanian Vol 12, No 1 (2005): Juni
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.59933

Abstract

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