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Endang Listyaningsih Suparyanti
Sebelas Maret University
Biochemistry, Genetics & Molecular Biology

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Potency of Moringa Leaf Extract (Moringa oleifera, Lam.) in Lowering the fatty infiltration of Heart on Hypercholesterolemia Rat (Rattus norvegicus). Raden Ismail Hafidh Adinugroho; Endang Listyaningsih Suparyanti; Muthmainah Muthmainah
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: The high consumption of high cholesterol foods associated with increased mortality from cardiovascular disease. Because of the side effects like myopathy and the high price of hipercholestrolemia therapy, public interest for the use of natural medicine increased. Moringa leaves contain flavonoids, polyphenols, and ?-sitosterol so that it can reduce fatty infiltration at heart. Histological Study of effect moringa leaves on heart has never been performed before. This study aimed to determine whether the extract of Moringa leaf (Moringa oleifera Lam.) can lower the fatty infiltration of heart on hypercholesterolemia rat model (Rattus norvegicus). Methods: This study was an experimental laboratory with post test only control group design. A sample of male rats were consisting of Sprague Dawley rats, weight 150-200 g, aged 2-3 months. The sampling technique used is incidental sampling. Twenty eight of samples were randomly divided into four groups, each group consisted of 7 rats.Group KK is given aquades. Group K (-), K1, and K2 is given a high-fat diet for 42 days. Group K1 and K2 were each given Moringa leaf extract at a dose of 200 and 400 mg pe kg of body weight from day 42nd until day 70th. Termination was performed on 71th day, then their hearts were prepared for specimen stained with hematoxylin eosin. Heart ventricle fat area was observed with 1000x magnification on light microscope. The data were analyzed using Kruskal-wallis comparative test and then Post-hoc test Mann Whitney was performed (? = 0.05). Results: The observation area of fat at left and right ventricle in KK, K (-), K1, K2 were 378.8153.32; 1280.15133.94; 386.7457.87; and 326.129.89 ?m. Kruskall-Wallis test showed significant result (p = 0.000). Mann Whitney test results showed no statistically significant difference in area of ventricle fat (p <0.05) among all groups, except between groups of KK and K1 (p = 0.633). Conclusion: The extract of leaves of Moringa (Moringa oleifera Lam.) can reduce steatosis / fatty liver histopathology picture rat heart (Rattus norvegicus) model of hypercholesterolemia. Keywords:Moringa leaf extract, heart, fatty infiltration, rat.
Effect of Ethanol Extract of Soursop (Annona muricata Linn.) Leaves on Intestinal Motility of Mice (Mus musculus) Induced by Oleum Ricini Agil Noviar Alvirosa; Setyo Sri Rahardjo; Endang Listyaningsih Suparyanti
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Diarrhea is one of major problem in the world. There are about 2 billion cases of diarrhea with a mortality rate of 1.5 million per year in the world. Diarrhea is the first cause of death in infants and toddler, while in all age groups is the fourth leading cause of death in Indonesia. One of the pathogenesis of diarrhea is an increase of intestinal motility. Leaves of soursop (Annona muricata Linn.) contains tannins, flavonoids and steroids which effect of intestinal antimotility. This study aims to prove that the ethanol extract of soursop leaves may inhibit intestinal motility in mice induced by oleum ricini. Methods: This study was a laboratory experimental with posttest only controlled group design. Samples were 30 Swiss Webster male mice, aged 2-3 months, and weight 20 g. The samples were divided into 5 groups, namely Negative Control Group (KKN), Positive Control Group (KKP), Treatment I Group (KP1), Treatment II Group (KP2), and Treatment III Group (KP3) were respectively given distilled water; loperamide HCl; ethanol extract of soursop leaves with dose of 5.6 mg/20 g BW; dose of 11.2 mg/20 g BW; and dose of 22.4 mg/20 g BW. After 60 minutes, all of mice were given oleum ricini and it was waited until 45 minutes. Then all of mice were given 10.0% activated carbon suspension in 3.0% pulvis gummi arabicum and left for 20 minutes. Furthermore, the mice were sacrificed by spinal dislocation and their intestines were removed. Length of intestine through by activated carbon marker was measured and compared to the length of intestine entirely. Data were analyzed by One Way ANOVA and Post Hoc Multiple Comparisons LSD (? = 0.05). Results: One Way ANOVA test results showed significant differences between the five groups with p = 0.000 (p < 0.05). The test results Post Hoc Multiple Comparisons LSD showed significant differences (p < 0.05) between KKN and KKP (p = 0.000), KKN and KP2 (p = 0.000), KKN and KP3 (p = 0.000); whereas there is no significant difference (p > 0.05) between KKP and KP3 (p = 0.810). Conclusion: The ethanol extract of soursop (Annona muricata Linn.) leaves can inhibit intestinal motility in mice (Mus musculus) induced by oleum ricini. Keywords: soursop leaves, oleum ricini, intestinal motility
Co-Authors Agil Noviar Alvirosa Muthmainah Muthmainah Raden Ismail Hafidh Adinugroho Setyo Sri Rahardjo