Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Current Biochemistry Jurnal Kompetitif BioEksakta : Jurnal Ilmiah Biologi Unsoed
Dodi Safari
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Current Biochemistry

 1 
Activity of Skin Secretions of Frog Fejervarya limnocharis and Limnonectes macrodon against Streptococcus pneumoniae Multidrug Resistant and Molecular Analysis of Species F. limnocharis Jajang Suhyana; I Made Artika; Dodi Safari
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia has about 450 frog species which is approximately 20% of frog species in the world. Among frog species found in Indonesia are Fejervarya limnocharis dan Limnonectes macrodon belonging to family Dicroglossidae. Frog skin secretion is considered to have a potency to be used as an alternative source of antibacterial agent against Streptococcus pneumoniae multidrug resistant (MDR). The aims of the present study were to analyze antibacterial activity of skin secretions of F. limnocharis and L. macrodon against S. pneumoniae multidrug resistant (MDR) and conduct molecular phylogenetic analysis of the frog used to ensure classification of frog species. The release of skin secretion was stimulated using epinephrine injection. Antibacterial activity of the skin secretions was tested using the well and paper disc methods. Results showed that skin secretions of F. limnocharis have antibacterial activity against S. pneumoniae multidrug resistant (MDR) SPN1307. The activity, however, was lower compared to that of chloramphenicol in both well and paper disc methods. On the other hand, skin secretions of L. macrodon failed to inhibit the growth of S. pneumoniae multidrug resistant (MDR) SPN1307. Molecular phylogenetic analysis was carried out on F. limnocharis based on DNA sequence of a partial fragment of mitochondrial cytochrome oxidase subunit I (COI) gene. Results showed that the frog F. limnocharis is closely related (97%) to Fejervarya sp from Bali. Skin secretions of F. limnocharis, therefore, has the potency to be developed as a source of antibacterial agents against S. pneumoniae multidrug resistant (MDR) SPN1307.
Co-Authors Daniel Joko Wahyono Eka Sevtia Mesta I MADE ARTIKA Jajang Suhyana Miranti Oviani