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Hasim Hasim
Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Biochemistry, Genetics & Molecular Biology Education

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Journal : current biochemistry

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Effect of Boiled Cassava Leaves (Manihot esculenta Crantz) on Total Phenolic, Flavonoid and its Antioxidant Activity Hasim Hasim; Syamsul Falah; Lia Kusuma Dewi
Current Biochemistry Vol. 3 No. 3 (2016)
Publisher : IPB University

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Abstract

Cassava leaves (Manihot esculenta Crantz) widely used as vegetables in many regions. Cassava leaves contain many minerals such as Fe, Zn, Mn, Cu, Mg, Ca, P, K, S, contain crude protein, β-carotene and have an active compound of flavonoids, phenolic, and contain chlorophyll which is a natural antioxidant. The aims of this study were to analyze the effect of boiled cassava leaves on total phenolics, flavonoids, and its antioxidant activity. Cassava leaves extraction was done by maceration method to obtain the methanol extract and infundation method to get the water extract. Based on phytochemical test, cassava leaves contained alkaloids, flavonoids, phenolic, tannins, and saponins. The highest levels of total phenolics and flavonoids contained in the methanol extract of simplicia, 30.57 mg GAE/g and 881.33 mg RE/g, respectively. Cassava leaves extract had potential as an antioxidant with the highest inhibition of DPPH radicals produced by the methanol extract of simplicia with IC50 values of 92.10 mg/L and followed by methanol extract of boiled leaves, water extract of simplicia, and water extract of boiled leaves with IC50 144.28, 155.76 dan 170.71 mg/L , respectively. In conclusion, the process of boiling cassava leaves could reduce total phenolic, total flavonoids and antioxidant activcity.
Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds Stefani Dhale Rale; Hasim Hasim; Syamsul Falah
Current Biochemistry Vol. 5 No. 3 (2018)
Publisher : IPB University

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This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.
Co-Authors Lia Kusuma Dewi Stefani Dhale Rale Syamsul Falah