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All Journal Current Biochemistry
Hasim Hasim
Department of Biochemistry, IPB University
Biochemistry, Genetics & Molecular Biology Education

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Antioxidant Activity, Inhibition α-Glucosidase of Cymbopogon nardus (L.) Rendle and Identification of Active Compounds Najmah Najmah; Hasim Hasim; Didah Nur Faridah
Current Biochemistry Vol. 8 No. 1 (2021)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.8.1.3

Abstract

Diabetes mellitus (DM) has become a health problem in various countries, even in Indonesia, multiple of Covid-19 sufferers have DM as comorbid. Treatment of DM can be conducted by delaying glucose intake performed by the α-glucosidase enzyme in the intestine. Treatment using synthetic drugs often causes side effects. Instead to overcome these problems, herbal medicine is a choice. Cymbopogon nardus (L.) Rendle served as a potential candidate as seen from prior research but related to DM drugs, further exploration needs to be performed. This study aims to acquire a DM medicine by using Cymbopogon nardus (L.) Rendle . The leaves were macerated using 96% concentration of ethanol followed by multilevel fractionation with n-hexane, water and ethyl acetate as solvents. The test results showed that the n-hexane fraction was the best for antioxidant activity and α -glucosidase inhibition with IC50 values of 8,23 ppm and 311,87 ppm while the identification of the compound by using Liquid Chromatograph-Mass Spectrometry (LC-MS/MS) suspected compounds that have a role were ar-turmerone, beesioside N and notohamosin A. Based on the test, the n-hexane fraction was found as a source of antioxidants as well as α -glucosidase inhibitors. Keywords: Antioxidant, α -glucosidase, Cymbopogon nardus (L.) Rendle, LC-MS/MS.
Antioxidant Activities and α-glucosidase Inhibition of Gayo Arabica Coffee Skin (Coffea arabica L) Baharuddin Yusuf Habiby Harahap; Hasim Hasim; Didah Nur Faridah
Current Biochemistry Vol. 8 No. 1 (2021)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.8.1.4

Abstract

This research objectives to determine antioxidant activities and inhibition of α-glucosidase 96 % ethanol extract of gayo arabica coffee skin, n-hexane fraction, ethyl acetate fraction, and water fraction. Gayo arabica coffee skin was extracted by ethanol using maceration and fractionation using n-hexane, ethyl acetate, and water. Quercetin and Gallic acid were used as standard to found total flavonoids and phenolics. Test of antioxidant activity using the DPPH method. After being tested the inhibition of the α-glucosidase IC50 ethanol extract, the n-hexane fraction, ethyl acetate fraction, and water fractions. The total flavonoids of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are 76.74, 99.07, 73.55, 50.24 mg QE/g extract . Total phenolics of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are respectively 211.85, 136.67, 145.19, 127.41 mg GAE/ g extract. Antioxidants activity of ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction are IC50 12.03 ppm, 36.08 ppm, 30.27 ppm, 11.62 ppm. Inhibition activity α-glucosidase enzyme respectively are 588.85 ppm, 341.81 ppm, 591.61 ppm, and 798.78 ppm. These showed that the ethanol extract and water fraction are the best in antioxidants and the ethanol extract and ethyl acetate fraction are the best in inhibiting the α-glucosidase enzyme. These results indicate that gayo arabica coffee skin extract has the potential to be developed as an antidiabetic compound. Keywords: Antidiabetic, Antioxidant, flavonoids, phenolics, α-glucosidase
Co-Authors Baharuddin Yusuf Habiby Harahap Najmah Najmah