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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Susan Maphilindawati Noor
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Veterinary

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The mucosal and systemic immune responses in chickens orally immunised with Campylobacter jejuni antigen entrapped in poly-lactide-co-glycolide microparticles Susan Maphilindawati Noor
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.249 KB) | DOI: 10.14334/jitv.v3i4.127

Abstract

An oral immunisation in chickens with antigen entrapping in biodegradable microparticles was evaluated in order to achieve optimal antibody responses following oral vaccination. This study was adapted to Campylobacter jejuni antigen in chickens to observe its stimulation both mucosal and systemic immune responses. A group of 5 embryonated chicken eggs was immunised with heat-killed C. jejuni entrapped in poly-lactide-co-glycolide (PLG) microparticles at day 17 of incubation deposited into the amniotic fluid. Seven days after hatching the chickens were oral boostered, these was design to as Group A. In the Group B, each embryonated egg was immunised with soluble C. jejuni as in the same as Group A. Immune responses of post vaccination were observed at day-14, the humoral immunity was evaluated with an ELISA and whereas mucosal antibody response was detected by fluorescent histology. The serum IgG and IgA antibody responses, and also the bile and intestinal scrapping IgA antibody responses to campylobacter in Group A were significantly higher than those of the soluble antigen of Group B (P<0.05). Total number of immunoglobulin-containing cells for IgG, IgM, and IgA isotypes in the ileum in Group A chickens were also significantly higher than those of Group B (P<0.05) but was not in the duodenum and spleen. Key words : Campylobacter jejuni, poly-lactide-co-glycolide microparticles, oral immunisation, chickens
Study in ovo immunisation with flagellin and whole cell protein antigens of Campylobacter jejuni in chickens Susan Maphilindawati Noor; Alan J Husband
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.048 KB) | DOI: 10.14334/jitv.v5i2.208

Abstract

In ovo immunisation of chickens with flagellin and whole cell protein antigens of Campylobacter jejuni was examined to determine Campylobacter infection. Four groups of embryonated chicken eggs (10 eggs per group) were immunised in ovo at day 17 of incubation and booster was given at 7 days post-hatch. Group I was immunised in ovo and oral booster with whole cell protein of C. jejuni, group II was immunised in ovo and oral booster with C. jejuni flagellin protein, group III was immunised in ovo and intraperitoneal booster with whole cell, and group IV was treated as control. The humoral immune responses were determined by enzyme-linked immunosorbent assay (ELISA) and the mucosal immune responses were examined by a direct fluorescent histology antibody technique. Immunised chickens of Group I, II, and III shown to have higher antibody titers than  those of control chickens (group IV). The titres of anti-campylobacter antibodies of all isotypes in serum, bile, and intestinal scrapping after challenge were not significantly different in all groups. In addition, when immunised chickens were orally challenged with a homologous strain of viable C. jejuni organism, the chickens remained infected throughout the experiment based on cloacal swabs and caecal contents. These findings indicated that although in ovo immunisation resulted in increasing of the mucosal and humoral immune responses in chickens, it is not strong enough to protect the Campylobacter colonisation in the intestinal tract.   Key words : In ovo immunisation, Campylobacter jejuni, whole cell, flagellar, chickens
Brucellosis: An Unrecognized Zoonotic Disease in Indonesia Susan Maphilindawati Noor
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.624 KB) | DOI: 10.14334/wartazoa.v16i1.867

Abstract

Brucellosis, a bacterial zoonosis, is a disease caused by members of the genus Brucella . In Indonesia, brucellosis has known as a contagious reproductive disease in animals, however, only certain people know that brucellosis can be transmitted to human . Bovine brucellosis is characterised by one or more of the following signs : abortion, retained placenta . orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk . The Brucella organism is transmitted to human most commonly by ingestion of untreated milk or milk products or through the mucous membranes and wound of the skin . The severity of human disease varies, depending largely upon the infecting strain . Brucella abortus, B . melitensis. B . suis and B. canis are highly pathogenic for humans . Clinical symptoms of human brucellosis may include an intermittent fever, headaches, weakness, arthalgia, myalgia and weight loss . Occasional complications include arthritis, endocarditis, hepatitis granuloma, meningitis, orchitis dan osteomyelitis have also been reported . Brucellosis can also produce spontaneous abortion in pregnant woman . Diagnosis is based on the isolation of the organism and serology . Antibiotics are usually the mainstay of treatment and long-term treatment may be required . Brucellosis can be controlled by comprehensive campaigns to eradicate the disease by vaccination programme followed by test and slaughter of domestic animals which exhibit positive serologic reactions to brucellae.   Key words : Brucellosis, zoonosis, diagnose, control
Efektivitas Ekstrak Biji Mangga Harumanis terhadap Staphylococcus aureus, Bacillus subtilis, Shigella sp., dan Escherichia coli (EFFECTIVITY OF MANGO HARUMANIS SEED EXTRACT TO STAPHYLOCOCCUS AUREUS, BACILLUS SUBTILIS, SHIGELLA SP., AND ESCHERICHIA COLI) Sri Suryatmiati Prihandani; Susan Maphilindawati Noor; Andriani .; Masniari Poeloengan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.274 KB)

Abstract

Mango (Mangifera indica L.) is a tropical fruit trees in the flowering plant. Mango has been reportedas an antibacterial. The experiment was conducted to analysis the secondary compounds in seed Mangiferaindica L. extract and to test the antibacterial of seed Mangifera indica L. Staphylococcus aureus, Bacillussubtilis, Shigella sp and Escherichia coli. The plants material was extracted by percolation with ethanol.The assays were performed by using paper diffusion for determination of inhibition zone and dilutionmethod for determination of minimum inhibitory concentration. The result showed that secondarycompounds in seed mango extract were flavonoid, triterpenoid, saponin. Seed mango extract hasantibacterial activity on Staphylococcus aureus, Bacillus subtilis, Shigella sp and Escherichia coli.
Co-Authors Alan J Husband Andriani . Masniari Poeloengan Sri Suryatmiati Prihandani