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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner Informatika Pertanian Jurnal Medik Veteriner Jurnal Kedokteran Hewan
Sri Suryatmiati Prihandani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Veterinary

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UJI DAYA ANTIBAKTERI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, Salmonella typhimurium DAN Pseudomonas aeruginosa DALAM MENINGKATKAN KEAMANAN PANGAN Prihandani, Sri Suryatmiati
Informatika Pertanian Vol 24, No 1 (2015): Juni 2015
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.762 KB) | DOI: 10.21082/ip.v24n1.2015.p53-58

Abstract

Garlic is widely used as a herbal medicine. In order to support the empirical use and improve its application in food safety, this study was conducted to test the antibacterial activity of garlic against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa) bacteria. Garlic was used in the form of fine powder. Garlic powder then dissolved in sterile distilled water and squeezed to obtain garlic solution with a concentration of 50%, 25% and 12.5%. Antibacterial activity of garlic juice was tested by diffusion method using paper disc to determine diameter of bacterial growth inhibition zones. Screening results demonstrated that the chemical constituents of garlic powder were saponins, flavonoids and triterpenoids. Garlic powder has antibacterial activity to Gram-positive S. aureus and Gram-negative bacteria E. coli, S. typhimurium and P. aeruginosa. Garlic has antimicrobial potential and can be used as decontaminant against Escherichia coli to maintain quality of food safety such as meat.
Serotype detection, molecular characterization and genetic relationship study on Pasteurella multocide local isolate Sri Suryatmiati Prihandani; Susan M Noor; Asmarani Kusumawati
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 2 (2017): JUNE 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.334 KB) | DOI: 10.14334/jitv.v22i2.1630

Abstract

Pasteurella multocide is a bacteria that causes snoring disease or Haemorrhagic Septicaemia (HS) in Indonesia with high mortality and morbidity in heterogeneous species including cattle as a source of animal products with high economic value. The complexity of conventional and biochemical identification is a major obstacle in the detection of this disease because P. multocide has five serotypes A, B, D, E and F, while serotype B is the leading cause of HS cases in Asia including Indonesia. Therefore, it is necessary to conduct a research that can determine the serotype and molecular characterization and genetic study of five isolates of P. multocide from Lampung and Kupang by Polymerase Chain Reaction (PCR) technique. After PCR was performed on specific genes, capsular genes, 16S rRNA genes, sequencing and analysis using Bioedit, BLASTn, CLUSTALW and MEGA7.0.25, it was found that the five isolates were divided into two serotype groups: A and B. Isolate P. multocide (code: PMc) from Lampung is high homolog with ATCC isolate 12945, so it can be used as a positive control serotype A in the detection of other P. multocide isolates with PCR. Whereas, isolate P. multocide from Kupang can be used as positive control of serotype B because it is identical to P. multocide PMTB2.1 (CP007205.2) from Malaysia that is isolated from buffalo infected by HS.
Efektivitas Ekstrak Biji Mangga Harumanis terhadap Staphylococcus aureus, Bacillus subtilis, Shigella sp., dan Escherichia coli (EFFECTIVITY OF MANGO HARUMANIS SEED EXTRACT TO STAPHYLOCOCCUS AUREUS, BACILLUS SUBTILIS, SHIGELLA SP., AND ESCHERICHIA COLI) Sri Suryatmiati Prihandani; Susan Maphilindawati Noor; Andriani .; Masniari Poeloengan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.274 KB)

Abstract

Mango (Mangifera indica L.) is a tropical fruit trees in the flowering plant. Mango has been reportedas an antibacterial. The experiment was conducted to analysis the secondary compounds in seed Mangiferaindica L. extract and to test the antibacterial of seed Mangifera indica L. Staphylococcus aureus, Bacillussubtilis, Shigella sp and Escherichia coli. The plants material was extracted by percolation with ethanol.The assays were performed by using paper diffusion for determination of inhibition zone and dilutionmethod for determination of minimum inhibitory concentration. The result showed that secondarycompounds in seed mango extract were flavonoid, triterpenoid, saponin. Seed mango extract hasantibacterial activity on Staphylococcus aureus, Bacillus subtilis, Shigella sp and Escherichia coli.
SEROLOGICAL DETECTION OF BOVINE LEPTOSPIROSIS IN SUKABUMI AND CIANJUR REGIONS Susanti, Susanti; Rachmawati, Faidah; Mulyadi, Andi; Sukatma, Sukatma; Purba, Hastuti Handayani S; Anastasia, Yessy; Ariyanti, Tati; Prihandani, Sri Suryatmiati
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.33751

Abstract

This study aims to detect leptospirosis serologically and serovars found in cattle in the Sukabumi and Cianjur areas. 165 cattle serum samples were taken randomly in the Sukabumi and Cianjur region. Microscopic Agglutination Tests (MAT) were used for serological testing using the antigens of Leptospira serovar Icterohaemorrhagiae, Javanica, Celledoni, Canicola, Ballum, Pyrogenes, Cynopteri, Rachmati, Australis, Pomona, Grippotyphosa, Hardjo, Bataviae, and Tarassovi. The MAT results showed that positive serum leptospirosis in cattle in Sukabumi region was 18.75%, with serovars Hardjo 16.25%, Tarassovi 1.25%, and Hardjo and Tarassovi (1.25%). Meanwhile, in Cianjur region, positive serum was 30.60% with serovar Hardjo 22.35%, Tarassovi 2.35%, Icterohaemorrhagiae 1.18%, Grippotyphosa 1.18%, Hardjo and Tarassovi 1.18%, Cynopteri and Hardjo (1.18%), Rachmati, Pomona, Bataviae and Tarassovi (1.18%). Leptospira serovar Hardjo was the dominant serovar in both regions. Overall, one serovar was detected in 22.42% of cattle, two serovars in 1.82% and four serovars in 0.61% cattle.
Genomic Characterization of Antimicrobial Resistance Genes in Pasteurella multocida Isolates from East Nusa Tenggara and Lampung, Indonesia Prihandani, Sri Suryatmiati; Wibawan, I Wayan Teguh; Noor, Susan Maphilindawati; Ekawasti, Fitrine; Khairullah, Aswin Rafif; Purba, Hastuti Handayani S.; Puarada, Alif Rahman Rohim; Safika, Safika
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.368-381

Abstract

Haemorrhagic Septicaemia (HS) is a devastating disease affecting cattle and water buffaloes in Indonesia, causing annual economic losses in livestock industries, particularly in Asia, Africa, and the Middle East. Pasteurella multocida, the causative agent of the disease, has shown increasing antimicrobial resistance, complicating treatment efforts. Therefore, this study aims to provide the first genomic analysis of P. multocida isolates from different Indonesian provinces, focusing on genes conferring resistance to beta-lactam antibiotics based on Whole Genome Sequencing (WGS) results. Genomic data can be used to confirm the results of phenotypic antibiotic resistance testing. P. multocida isolates analyzed in this study were sourced from the Indonesian Research Center for Veterinary Science (IRCVS) collection, which included 2 samples originating from different geographic locations within Indonesia. The samples were subjected to biochemical, molecular, and antimicrobial susceptibility testing. WGS was performed using Oxford Nanopore Technologies (ONT) with subsequent bioinformatics analysis for genome assembly and resistance profiling. Phenotypic analysis showed significant variations between the isolates. The NTT isolate showed resistance to Penicillin, while the Lampung 2952 remained susceptible in the disk antibiotic test. Genome sequencing revealed extensive resistance determinants, including β-lactamase genes (blaZ, blaR1) in the NTT isolate, correlating with its phenotypic resistance. The high-quality genome assemblies (N50: 17,225 bp for NTT; 12,662 bp for Lampung 2952) enabled comprehensive resistome characterization, identifying more than 22 resistance genes in each isolate, including novel variants not previously reported in Indonesian strains. Therefore, this study provides the first genomic and resistance analysis based on Whole Genomic Sequencing data of Indonesian P. multocida isolates. The results show the urgent need for enhanced surveillance and prudent antimicrobial use in livestock management.
Co-Authors Andi Mulyadi Andriani . Asmarani Kusumawati Fitrine Ekawasti, Fitrine I wayan Teguh Wibawan Khairullah, Aswin Rafif Masniari Poeloengan Puarada, Alif Rahman Rohim Purba, Hastuti Handayani S Purba, Hastuti Handayani S. Rachmawati, Faidah Safika S, Safika Sukatma Sukatma Susan M Noor Susan Maphilindawati Noor Susan Maphilindawati Noor Susanti Susanti Tati Ariyanti Yessy Anastasia, Yessy