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All Journal Jurnal Veteriner Jurnal Biologi Udayana Biotropika Jurnal Ilmu Ternak Veteriner Phenomenon : Jurnal Pendidikan MIPA BIOMA Abdimasku : Jurnal Pengabdian Masyarakat

R Susanti

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Author-ID : 2823408

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Physics Social Sciences Veterinary

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Waterfowl potential as resevoirs of high pathogenic avian influenza H5N1 viruses R Susanti; R.D Soejoedono; I-G.N.K Mahardika; I-W.T Wibawan; M.T Suhartono
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The high population of waterfowl subsequently with the high case fatality of poultry and people in West Java regency caused by HPAI H5N1 can raise possibility that waterfowl was a natural reservoir. This research aimed to prove that waterfowl in West Java served as reservoir of AI virus (primarily H5N1) and also identify the virus pathotype based on cleavage site of amino acid sequence. Cloacal swab sample was obtained from healthy and unvaccinated waterfowl from Sukabumi and Bogor Regency. Cloacal swab was propagated in 9 days old embryonic chicken eggs. Allantoic fluid was harvested at the 4th day of incubation and then tested for hemagglutination, and positive isolate continued with virus sub-typing using PCR method. H5 gene from H5N1 isolate then sequenced using dideoxy termination method. Multiple alignment of nucleotide sequences were analysed using MEGA-3.1 program. Sub-typing using PCR method indicated the existence of 25 strain H5N1, 16 strain HxN1, 4 strain H5Nx and 9 virus ND. Characterization of cleavage site amino acid sequence indicated that all H5N1 sample were pathogenic with sequence QRERRRKKR (23 sample) dan QRESRRKKR (2 sample). Waterfowl was HPAI H5N1 virus reservoir. Asymptomatic infection in waterfowl, but the virus shedding gradually occurred and therefore it became potential source of H5N1 virus infection. Our findings suggest that immediate action is needed to prevent the transmission of highly pathogenic avian influenza viruses from the apparently healthy waterfowl into terrestrial poultry or human. Key Words: HPAI, H5N1, Reservoir, Water Fowl

Molecular analysis of Hemagglutinin Gen of Highly Pathogenic Avian Influenza of H5N1 Subtype Isolated from Waterfowls R Susanti; Retno D Soejoedono; I Gusti Ngurah K Mahardika; I Wayan T Wibawan; Maggy T Suhartono
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Avian influenza viruses (AIV) subtype H5N1 isolated from waterfowls in West Java pose the known characteristic of highly pathogenic strains, with polybasic amino acid sequence of cleavage site QRERRRKKR and QRESRRKKR. This research aimed to analyze the important domains of hemagglutinin (HA) gene of those isolates. Fragment of HA gene was amplified using RT-PCR method with specifically-designed primer pairs and sequenced using dideoxy termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA-3.1 program. Some of biological domains of HA, i.e. antigenic sites, receptor binding pocket, and glycosylation sites of the isolates were polymorphic. The viruses also pose conserved glutamine (Q) and glisin (G) residues at the known receptor binding site, at the position 222 and 224 respectively. This findings clearly show that all AIV subtype H5N1 isolaed from waterfowl preservesthe α-2,3NeuAcGal avian receptor specificity. Key Words: Antigenic Sites, Glycosylation Sites, Receptor Binding Pocket, AIV H5N1, Waterfowls

Co-Authors Ani Julita Br Sipayung Ari Yuniastuti Devi Dwi Jayanti Endah Peniati Hidayat Trimarsanto I Gusti Ngurah K Mahardika I Gusti Ngurah Kade Mahardika I Wayan Suardana I Wayan T Wibawan I wayan Teguh Wibawan I-W.T Wibawan Krispinus Pukan M.T Suhartono Maggy T Suhartono Maggy Thenawidjaja Suhartono Mohamad Ikhsan Nurulloh Nur Kusuma Dewi R.D Soejoedono Retno D Soejoedono Retno Damajanti Soejoedono Sri Sukaesih Talitha Widiatningrum Yustinus Ulung Anggraito

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Journal : Jurnal Ilmu Ternak Veteriner