Sri Poernomo
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Distribution of Escerichia coli serotype OIKi, 02Kt, and O7sK8o in poultry in Indonesia Sri Poernomo; E. Juarini
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 3 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.243 KB) | DOI: 10.14334/jitv.v1i3.34

Abstract

Fowl colibacillosis or colisepticaemia is commonly caused by Eschercia coli serotypes OIKI, 02Kt, and O7sKgo . To understand the distribution of these three serotypes of E. coli in poultry breeding farms in Indonesia, samples were collected from surveyed areas of Jakarta, Bogor, Tengerang, Bekasi and Sukabumi, West Java, Central Java, East Java, Bali, North Sumatera, Lampung, West Kalimantan and South Sulawesi . A total of 2,454 samples which consisted of 1,413 organs of sick birds, 398 feed, 336 drinking water and 307 litter, were collected throughout the surveyed areas . The specimens were then examined bacteriologically toward the presence of E. coli . As many as 950 E. coli isolates consisting of 85 (9 .0%) OIKI ; 489 (51 .5%) 02KI ; 101 (10.6%) 078K8o, and 275 (28.9%) of other serotypes were isolated from the samples .
Isolation of Pasteurella multocida from broiler chickens Sri Poernomo; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 2 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.839 KB) | DOI: 10.14334/jitv.v2i2.59

Abstract

Pasteurella multocida, the etiological agent of fowl cholera, was isolated from five, 32 days oldbroilerchickens in the late of 1992. The chickens were from a farm located in Bogor area, raised in cages and each flock consisted of 1,550 broilers . Therewere 230 birds, aging from 28-31 days old, died with clinical signs of lameness and difficulty in breathing. Serological test of the isolate revealed serotype Aof Carter classification . To prove  its virulences, the isolate was then inoculated into 3 mice subcutaneously. The mice died less then 24 hours postinoculation and P. multocida can be reisolated . The sensitivity test to antibiotics and sulfa preparations showed that the isolate was sensitive to ampicillin, doxycyclin, erythromycin, gentamycin, sulfamethoxazol-trimethoprim and baytril, but resistance to tetracyclin, kanamycin and oxytetracyclin. This is the first report of P. multocida isolation in broiler chickens in Indonesia, and it is intended to add information on bacterial diseases in poultry in Indonesia.   Keywords : Pasteurella multocida, isolation, broiler chickens, antibiotics
Haemophilus paragallinarum in chickens in Indonesia : II . Physiological and biochemical properties of Haemophilus spp . from diseased chickens Sri Poernomo; Sutama .; Yapto Nazrudin
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.386 KB) | DOI: 10.14334/jitv.v2i4.82

Abstract

A total of 46 isolates of Haemophilus spp . consisted of 42 local isolates from diseased chickens and four reference strains of Haemophilus paragallinarum were examined for their physiological and biochemical properties . These isolates consisted of 2 groups. One group consisted of 21 local isolates and reference strains of H. paragallinarum were catalase negative and identified as Haemophilus paragallinarum and they did not ferment galactose and trehalose . The other group was catalase positive and suspected as Haemophilus avium . This catalase positive group of these 21 avian Haemophili should be examined with other carbohydrates in order to be identified accurately .   Keywords : Haemophilus spp ., physiological, biochemical, chickens
Phage typing and sensitivity test to antibiotics of Salmonella enteritidis isolates from Indonesia Sri Poernomo; Adin Priadi; Lily Natalia
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.499 KB) | DOI: 10.14334/jitv.v11i2.521

Abstract

Salmonella enteritidis (SE) is frequently implicated in disease outbreaks such as human food poisoning. Phage typing have been proved to be a valuable and sensitive tool in the control of SE infections. The ability of phage to distinguish varieties among apparently identical serotypes led to the development and acceptance of phage typing as a significant epidemiological procedure. To determine the epidemiological pattern of SE, phage typing of 53 SE isolated from various sources in Indonesia during 1991–1999, has been conducted using 16 typing phages of phage typing scheme of SE obtained from the International Collaborating Center for Enteric Phage typing, Central Public Health Laboratory, Colindale, UK. The lyse blood isosensitest was then used to test the sensitivity of the Salmonella isolates to antibiotics. The phage typing results obtained that of 53 Salmonella isolates there were one S. infantis, one S. berta, and 46 SE phage type 4, 2 SE phage type 7 (from chicken and water), 1 SE phage type 6 (from chicken) and 2 SE phage type 1 (from chicken). SE phage type 4 isolates comprised of 2 isolates from human, 19 isolates from chicken (young and adult), 17 isolates from day old chicks, 4 isolates from fluff, 2 isolates from chicken meat, 1 isolate from poultry farm water, 1 isolate from dog organ. These findings indicated that contaminated chicken appeared to be the sources of human and dog for SE infection. The results of sensitivity test of the isolates to antibiotics showed that most of the Salmonella isolates from Indonesia were resistant to the antibiotics tested. Key Words: Salmonella Enteritidis, Phage typing, Sensitivity test, Indonesia, Chicken
Haemophilus paragallinarum in chickens in Indonesia: III. Antimicrobial drug sensitivity test ofHaemophilus paragallinarum from chickens suffering of coryza Sri Poernomo; Sutarma Sutarma; Sang Ayu Ketut Dewi Silawatri
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.846 KB) | DOI: 10.14334/jitv.v2i4.83

Abstract

An agar disc diffusion method was used to examine the sensitivity of 27 Haemophilus paragallinarum (Hpg) isolates consisted of 23 local isolates, 4 standard isolates (serotype A) and Escherichia coli ATCC 24922 as a control to eight antimicrobial drugs (ampicillin, erythromycin, oxytetracycline, doxycycline, neomycin, streptomycin, colistine and sulphanlethoxazole-trimethoprim) . Twenty one out of 23 local isolates of Hpg were sensitive to doxycycline, 19 isolates to ampsllin, 18 isolates to oxytetracycline, 17 isolates to sulphametoxazole-trimethoprim, 16 isolates to erythromycin, and 13 isolates to neomycin, while 13 isolates were resistance to colistine and 11 isolates were also resistance to streptomycin .   Keywords : Haemophilus paragallinarum, chicken, coryza, sensitivity test, antimicrobial drug
Salmonella Enteritidis infection in young broiler chickens from breeding farm: A case report Sri Poernomo; Indrawati Rumawasz; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.046 KB) | DOI: 10.14334/jitv.v2i3.70

Abstract

old from a breeding farm not far from Bogor. Samples were examined pathologic anatomically (PA) and bacteriologically to isolate the causative agents . The sensitivity of the main causative agents isolated from the samples was tested with some drugs, while its pathogenicity was tested in 3 days old chickens intramuscularly, subcutaneously, intraperitoneally and orally, three chickens per inoculations . Exudative and caseous omphalitis, pericarditis, hepatitis, sirsacculitis, and coxofemoral and knee joints were observed in PA examinations, while on bacteriological examination the main cusative agent, ie. Salmonella Enteritidis was isolated successfully . Drug sensitivity test showed that the pathogen was sensitive to chloramphenicol, baytril, gentamisin, and sulphametoxazole-trimethoprim, and resistant to erythromycin, colistin, streptomycin and kanamycin . On the other hand, pathogenicity test of the isolate showed that all but two chickens which were inoculated orally, were died 24 hours post-inoculation . It was concluded that young broiler chickens of the farm were infected by Salmonella Enteritidis.   Keywords : Salmonella Enteritidis infection, broiler chickens, breeding farm
Infectious Coryza (Snot) in Chicken in Indonesia Anni Kusumaningsih; Sri Poernomo
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 10, No 2 (2000)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.434 KB) | DOI: 10.14334/wartazoa.v10i2.744

Abstract

Infectious coryza (snot) is an infectious disease of poultry, especially chicken, caused by Haemophilus paragallinarum (Hpg). The bacterium is small gram negative, non-motile, microaerophilic, and nicotinamide adenine dinucleotide (NAD) dependent. In Indonesia, 3 serotypes of Hpg, A, B and C have been isolated. The course of the disease is usually about 1-3 weeks. In poultry farms with vaccination program, the mortality rate is very low, about 0-5%, but morbidity rate can reach 30-40% with loss of egg production up to 10-50%. Disease control with vaccination using a bivalent (A and C serotypes) or trivalent (A, B and C serotypes) killed vaccine is practiced. The treatments are carried out with drugs including antibiotics and sulpha preparation. Result of antibiotic sensitivity test of Hpg isolates shown that mostly of these isolates were resistance to colistin and streptomycin.   Key words : Infectious coryza, Haemophilus paragallinarum, chicken