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Bioactive substances of some herbals and their effectiveness as antioxidant, antibacteria and antifungi Arnold P. Sinurat; Elizabeth Wina; Susana I.W. Rakhmani; T. Wardhani; Tuti Haryati; T. Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 1 (2018): MARCH 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was conducted to explore the bioactive substances of some local plants in order to find their effectiveness as antioxidant, antibacteria and antifungi to be used as feed additives. Twelve plants material were used in this study. The total phenol, tannin and saponin contents in the plant extract were assayed. The extracts were also assayed on their antioxidant activities and on their ability to depress in vitro gas production of microbes obtained from chicken’s guts, their ability to inhibit growth of bacteria (E. coli and Salmonella enteridis) and fungi (A. niger). The results showed that the highest total phenol and total tannin contents were found in clove leaf extract, while the highest saponin content was found in Sapindus rarak fruit pericarp. The highest antioxidant activity was found in the leaffruit extract. Gas produced by microorganims was reduced to the level similar to antibiotic addition were found with addition of hexane- extract of leaffruit, kapok seed or methanol- extract of mangosteen fruit rind pulp or clove leaves. The best inhibitory effect on E. coli growth (measured by clearing zone) was found in methanol extract of S. rarak fruit. However, the most effective growth inhibitor for both E. coli and Salmonella was the liquid smoke of cashew nut shell. The best growth inhibitor for fungal growth was found in extract of clove leaves. Therefore, clove leaves extract (anti fungi), liquid smoke of cashew nut shell (antibacteria) and leaffruit (antioxidant) may have potential to produce feed additives to substitute antibiotic growth promoters.

Performance of EPMp broiler ducks feed with various levels of dietary lysine up to 10 weeks of age Maijon Purba; Arnold P. Sinurat
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 1 (2017): MARCH 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Determining the optimum level of lysine in the ration will be a significant effort to avoid poor growth in duck husbandry. The purpose of this study was to determine the optimum dietary lysine requirement for EPMp broiler ducks, raised up to 10 weeks of age. The study was designed in a completely randomized design (CRD) with 4 dietary treatments, and 4 replicates. Each replicate consisted of 10 ducks. The 4 treatments were diets, containing lysine: 0.60% (T1); 0.70% (T2); 0.80% (T3) and 0.90% (T4). Variables measured included: feed consumption, body weight gain, feed conversion ratio (FCR), carcass and carcass cuts percentages. The results showed that the performance of 10 weeks of age EPMp broiler duck was significantly affected by the level of dietary lysine (P<0.05). The average body weight gain and the FCR of the duck were significantly affected (P<0.05) by dietary lysine level, but not for feed consumption and percentage of carcass (P>0.05). The average body weight gain of EPMp duck with T4 treatment (0.90% lysine) was significantly higher than that T1 (0.60% lysine) but did not significantly difference (P>0.05) with T2 and T3. The average FCR of EPMp duck under T3 and T4 were significantly (P<0.05) higher than that of EPMp duck under T1. It was concluded that the optimum dietary lysine to produce maximum body weight gain of EPMp duck raised up to 10 weeks of age was 0.70%, while for minimum FCR were at the level of 0.80% and 0.90%.

Effectivity of BS4 enzyme complex on the performance of laying hens fed with different ingredients Arnold P. Sinurat; Tresnawati Purwadaria; Tuti Haryati
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was carried out to evaluate the effectivity of an enzyme complex produced by Eupenicilium javanicum BS4 on the performance of laying hens fed with different feed ingredients. Three diets were formulated with similar nutrients (protein, ME, digestible amino acids, Ca and available P) value to meet the nutrient requirement of laying hens. Diets were formulated based on maize, palm kernel cake (PKC) or rice bran. The diets were either supplemented or not with BS4 enzyme complex and fed to Isa Brown pullet from 19 to 37 weeks old. Each diet was fed to 24 birds and the performances were recorded. Data were analysed statistically with 2 X 3 factorial design with six replicates. Results showed that supplementation of BS4 enzyme into laying hens diet did not influence body weight change, feed intake, mortalities, egg weight and egg shell thickness but significantly increased egg production (P<0.05) and the FCR (P<0.01) in any feedstuff tested. It also improved egg yolk colour score on diet contained 20% PKC. Laying hens fed 20% PKC produced more eggs than those fed maize or rice bran. The egg shell of laying hens fed rice bran were thicker than those fed with maize or PKC. It is concluded that BS4 enzyme complex is effective when supplemented into laying hens diet.

Carbohydrate and protein digestion on palm kernal cake by Mannanase BS4 and papain cocktail enzymes SIW Rakhmani; Y. Pangestu; Arnold P. Sinurat; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Supplementation of the mannanase BS4 enzyme on palm kernel cake (PKC) increased its metabolisable energy (ME), and supplementation with protease is expected to increase its protein digestibility. Therefore, the purpose of this research is to determine the optimum proportion of cocktail enzymes between BS4 β-mannanase (produced by Eupenicillium javanicum) and protease (papain) and their degradation activities on carbohydrate and protein of PKC. The β-Mannanase was produced by the mold through solid substrate fermentation (SSF) using coconut meal as the substrate. The papain was extracted from papaya latex (PL), collected by longitudinal incisions on unripe papaya fruit and oven dried overnight. The evaluation of enzyme cocktails for PKC hydrolysis was done at pH 5.8 and 40°C which are similar with poultry intestine condition and both enzymes are still active. The β- mannanase BS4 : papain were mixed with some proportions, i.e.: 100 : 0; 75 : 25; 50 : 50; 25 : 75 and 0 : 100% (by volume) in order to study the optimum cocktail composition ratio. The activities of β-mannanase towards gum locust bean was 86 U.ml-1 and papains PL activity was 18,000 U.g-1respectively. PL was chosen for synergistic reaction and compared with a commercial Merck papain (CMP, 20,000 U.g-1) as positive control. β-Mannanase BS4 showed carbohydrate digestion activity, and protein digestion activity was not detected. Papain showed protein digestion activity and no carbohydrate digestion activity. Enzyme cocktails of 50 : 50 from PL protease showed slightly increased in synergistic protein digestion activity in PKC. However, its reduction sugar production was much lower than 100 : 0 and 75 : 25. Amino acids production by enzyme cocktails 75 : 25 were higher than that of 100 : 0. As a result, the best volume composition of β-mannanase BS4 and papaya latex was 75 : 25 (v:v) or 14 : 10 (U:U).Key Words: Mannanase BS4, Papain, Cocktail-Enzymes, Palm Kernel Cake

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