Elizabeth Wina
IRIAP

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Bioactive substances of some herbals and their effectiveness as antioxidant, antibacteria and antifungi Arnold P. Sinurat; Elizabeth Wina; Susana I.W. Rakhmani; T. Wardhani; Tuti Haryati; T. Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 1 (2018): MARCH 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.936 KB) | DOI: 10.14334/jitv.v23i1.1660

Abstract

A study was conducted to explore the bioactive substances of some local plants in order to find their effectiveness as antioxidant, antibacteria and antifungi to be used as feed additives. Twelve plants material were used in this study. The total phenol, tannin and saponin contents in the plant extract were assayed. The extracts were also assayed on their antioxidant activities and on their ability to depress in vitro gas production of microbes obtained from chicken’s guts, their ability to inhibit growth of bacteria (E. coli and Salmonella enteridis) and fungi (A. niger). The results showed that the highest total phenol and total tannin contents were found in clove leaf extract, while the highest saponin content was found in Sapindus rarak fruit pericarp. The highest antioxidant activity was found in the leaffruit extract. Gas produced by microorganims was reduced to the level similar to antibiotic addition were found with addition of hexane- extract of leaffruit, kapok seed or methanol- extract of mangosteen fruit rind pulp or clove leaves. The best inhibitory effect on E. coli growth (measured by clearing zone) was found in methanol extract of S. rarak fruit. However, the most effective growth inhibitor for both E. coli and Salmonella was the liquid smoke of cashew nut shell. The best growth inhibitor for fungal growth was found in extract of clove leaves. Therefore, clove leaves extract (anti fungi), liquid smoke of cashew nut shell (antibacteria) and leaffruit (antioxidant) may have potential to produce feed additives to substitute antibiotic growth promoters.
Chitosan nanoparticle of hCG (Human Chorionic Gonadotrophin) hormone in increasing induction of dairy cattle ovulation Fitra Aji Pamungkas; Ria Sari Gail Sianturi; Elizabeth Wina; Diana Andrianita Kusumaningrum
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.19 KB) | DOI: 10.14334/jitv.v21i1.1343

Abstract

A controlled release delivery system of human chorionic gonadotropin (hCG) hormone helps to overcome the rapid degradation of hCG hormone in the blood, to avoid the use of multiple injections for ovulation induction and to enhance reproductive efficacy. This study aimed to prepare chitosan nanoparticles hCG (CS-NPh) and to determine its efficacy as nasal spray of CS-NPh. The observed parameters include physico-chemical characteristics of CS-NPh and the follicle size, corpus luteum, the time of ovulation and onset of estrus performed after administration of CS-NPh as a nasal spray compared with intramuscular hCG (control) at a dose of 1,000 IU in dairy cattle. The result showed that the formation of the hormone hCG nanoparticles is still in the size range of nanoparticles with a well and more stable molecular mass distribution, so it can be used as a carrier component of hormones. The result showed that the time of ovulation after hCG by intramuscular (day to 3.13±0.35) and CS-NPh as a nasal spray (days to 3.33±0.49) with the follicle size by 1.62±0.22 and 1.76±0.28 cm showed no significant differences (p> 0.05), likewise the size of the corpus luteum and onset of oestrus. This indicates that administration of CS-NPh as a nasal spray can be used in enhancing the induction of ovulation in dairy cattles.Key Words: Nanoparticles, hCG, Nasal Spray, Ovulation
Synergistic Effect of Biofat and Biochar of Cashew Nutshell on Mitigate Methane in the Rumen Andi Saenab; Komang G Wiryawan; Y Retnani; Elizabeth Wina
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 3 (2020): SEPTEMBER 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i3.2475

Abstract

The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 treatments and 4 replications. A series of biofat (BF) and biochar (BC) combination were added each to substrate as followed BFBC1 = 0: 100%; BFBC2 = 25:75%; BFBC3 = 50:50%; BFBC4 = 75:25%; BFBC5 = 100: 0%. While, a series of biofat (BF) and biosmoke (BS) combination as followed BFBS1 = 0: 100%; BFBS2 = 25:75%; BFBS3 = 50:50%; BFBS4 = 75:25%; BFBS5 = 100: 0%. Both series used a control treatment which contained only substrate. The in vitro experiment was repeated 4 times and each treatment was done in duplicates. The measured variables were: total gas and CH4 productions, dry matter, organic matter, NDF degradability, NH3 and partial VFA concentrations. The results showed that the combination of biofat and biochar levels resulted in a significant decrease (P