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H.A. Hassim
UPM
Veterinary

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Histological changes of liver tissue and serobiochemical relation in does with pregnancy ketosis A.F.M. Azmi; A.A.A. Ghani; A.S. Saadan; A. Mokrish; K.S. Lai; M. Zamri-Saad; A.B. Zuki; H.A. Hassim
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 2 (2016): JUNE 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.17 KB) | DOI: 10.14334/jitv.v21i2.1357

Abstract

Histological changes of liver in does with pregnancy ketosis were characterized. Twenty pregnant does at day 80 of pregnancy were used for this experiment. A total of 10 does were fed by grass (Napier) and goat concentrate with water ad libitum. Those 10 goats considered as healthy pregnant goat, and another 10 goats showing clinical and subclinical signs of ketosis considered as unhealthy pregnant does. Liver biopsies were performed when clinical signs appeared. Beta-Hydroxybutyrate (BHBA), free fatty acid (FFA), and glucose were dosed. Histological preparation revealed similar incidence and intensity of mild liver steatosis with lower cellular vacuolation in hepatocyte presence in healthy late pregnant does. Almost all of the pregnant does with ketosis state (n=8/10) had large amount of small lipid droplets in almost every hepatocyte over the whole liver acinus with higher number of cellular vacuolation, and related with higher BHBA and FFA levels while low in glucose level.
Determination of fibernolytic enzyme activities of white rot fungi isolated from oil palm fronds Mohammad Azri bin Azmi; S. Alias; A.F.M. Azmi; A.A.A. Ghani; M.S. Shahudin; Y.M. Goh; M.M. Noordin; M.T. Yusof; Z. Zunita; H.A. Hassim
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 2 (2016): JUNE 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.822 KB) | DOI: 10.14334/jitv.v21i2.1362

Abstract

Oil palm fronds (OPF) is widely used as the source of roughage for the farm animals. However, the full potential of OPF as animal feed is limited by their high lignin content which limits the rumen microbe’s access to the cellulose and hemicellulose. White rot fungi (WRF) are a group of fungi belonging to basidiomycete phylum and are commonly found in decaying woody plant. They possess the ability to degrade lignin. This experiment aims to identify the phylum of the best lignin decaying fungi based on their enzymes activity. In this experiment, 11 fungi species were isolated from decaying oil palm fronds. They are labelled as WR1, WR 2, WR3, WR4, WR5, WR6, WR7, WR8, WR9, WR10 and WR11. Their fibernolytic enzyme activities which includes laccase, manganese peroxidase, lignin peroxidase, avicelase, carboxylmethylcellulase and xylanase are analysed using the solid state fermentation method. It is was found that 5 fungi species which are the WR1, WR2, WR4, WR7 and WR10 produced the highest ratio of lignin degrading enzyme to cellulose and hemicellulose degrading enzyme. The fungi are then analysed under microscope to determine the phylum of the fungi. From the observation, the fungi are identified to belong to the phylum basidiomycetes due to presence of clamp connection.
Co-Authors A. Mokrish A.A.A. Ghani A.B. Zuki A.F.M. Azmi A.S. Saadan K.S. Lai M. Zamri-Saad M.M. Noordin M.S. Shahudin M.T. Yusof Mohammad Azri bin Azmi S. Alias Y.M. Goh Z. Zunita