A. P. Sinurat
Indonesian Research Institute for Animal Production

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Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper Tuti Haryati; A. P. Sinurat; B. Listian; H. Hamid; T. Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 4 (2016): DECEMBER 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.318 KB) | DOI: 10.14334/jitv.v21i4.1524

Abstract

Cellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments,  i.e., 30% manure (M30); 15 %  manure + 15 % paper waste  (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P<0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production. 
Performances of KUB Chickens Fed Diets with Different Nutrient Densities and BS4 Enzyme Supplementation A. P. Sinurat; T. Haryati; A. Herliatika; N. Pratiwi
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.73

Abstract

An experiment was conducted to study the effect of dietary enzyme supplementation (E) on the performance of KUB chickens fed different nutrient densities (ND). Diets with three densities: 70.7 g crude protein/Mcal or high (H), 66.1 g crude protein/Mcal or medium (M), and 59.3 g crude protein/Mcal or low (L), were formulated and supplemented with or without enzyme. Diets were given in four feeding programs, i.e., H-M-L, H-M-M, M-M-L, and M-L-L during the starter (1–28 d), grower (29–56 d), and finisher (57–84 d) periods, respectively. Each treatment was replicated five times. Bodyweight gain (BWG), feed intake, and FCR were measured each period. At the end of the trial, carcass yield and internal organs were measured. Results of the experiment (1–84 d period) showed that the feed intake was significantly affected by ND. Chickens fed the H-M-L diets have the highest feed intake, while the lowest was found in chickens fed M-M-L diets. A significant interaction was found in the FCR. The best FCR was found in chickens fed the H-M-M diets without enzymes, but the best FCR was found on the M-M-L diets with enzymes. Livability, carcass yield, abdominal fat, liver, proventriculus, and gizzard weights were not affected by the treatments. The jejunum sizes of chickens were significantly longer when fed the low-density diet than those fed the higher nutrient density diet. The ileum sizes of chickens were significantly shorter than chickens fed the diet without enzymes. The highest income over feed cost was achieved when chickens were fed the M-M-L diets supplemented with enzymes. It is concluded that the best performance of growing KUB chickens was obtained when fed M-M-L diets supplemented with BS4 enzymes (30 Units of saccharification/kg diet) and when fed H-M-M diets without enzyme supplementation.