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Tuti Haryati
Indonesian Research Institute for Animal Production
Veterinary

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Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper Tuti Haryati; A. P. Sinurat; B. Listian; H. Hamid; T. Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 4 (2016): DECEMBER 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.318 KB) | DOI: 10.14334/jitv.v21i4.1524

Abstract

Cellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments,  i.e., 30% manure (M30); 15 %  manure + 15 % paper waste  (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P<0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production. 
Optimization of BS4 Enzyme production with Different Substrate Thickness and Type of Trays Tuti Haryati; Arnold P Sinurat; H Hamid; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 4 (2019): December 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i4.2055

Abstract

BS4 enzyme that is produced from solid substrate fermentation (SSF) on coconut cake with Eupenicillium javanicum BS4 in tray bioreactor has been applied as a feed additive. It increases the nutritional value of animal feedstuff. The BS4 production on SSF may be influenced by the better aeration through the perforated trays or by the thinner substrate. The aim of this research is to optimize the production of BS4 with different substrate thicknesses and types of trays. The trial was carried out using a factorial randomized design (2x2x3) with 6 replicates. The first factor was the type of trays: i.e., non-perforated and perforated tray. The second factor was the thickness of the substrate: i.e., 1.5 and 3.0 cm, while the third factor was the duration of fermentation: i.e. 5, and 7 days. The variables observed were moisture content, dry matter loss (DML), mannanase and saccharification activities, soluble protein content, their specific activities, and yield. Statistical analysis showed no interactions between the three factors, but there were interactions between types of trays and substrate thicknesses, as well as type of trays and incubation times on the mannanase activity and yield of mannanase. The results showed that DML was observed on day 7 were around 31.43- 36.89. The highest mannanase activity was observed on the non-perforated tray with 3 cm thickness on day 7. The saccharification activity towards palm kernel meal was better in the non-perforated tray on day 7 but not influenced by The yield value of mannanase and saccharification activities on a non-perforated tray with 3.0 cm thickness on day 7 was also the highest. Based on energy efficiency and the cost of production, it can be concluded that the optimum condition to produce the BS4 enzyme was observed in the non-perforated tray with 3 cm thickness and fermented for 7 days.
Effectivity of Dry and Liquid BS4 Enzymes in Improving Performance of Broiler Chickens Fed Different Nutrient Density Diet Arnold Parlindungan Sinurat; Tuti Haryati; Nurul Pratiwi; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 2 (2022): June 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i2.3051

Abstract

Supplementation of enzymes in feed is now commonly practiced to increase the nutrient availability of feed and the performance of poultry. A new enzyme called BS4 was produced by cultivating Eupenicilium javanicum. It is necessary to test the efficacy of this enzyme since the effectiveness of enzyme supplementation depends on many factors. An experiment was conducted to study the effect of dietary BS4 enzyme supplementation in improving the performance of broiler chickens. A number of 300 broilers DOC was distributed into 30 pens and reared until 35 d. Six experimental diets i.e., factorial of 2 (Standard diet, and low nutrient density diet) X 3 (Control, BS4 liquid enzyme, and BS4 powder enzyme) were formulated with 5 replications. The performance (feed intake, body weight, FCR, and survival rates) were observed during the starter (1-21 d) and whole (1-35 d) periods. At the end of the trial, measurements were also made on the carcass yield, abdominal fat, liver, and gizzard weights. Results showed that performances of broilers from 1-35 d were not significantly affected by interaction between nutrient density and enzyme supplement. The nutrient density also did not affect performances of broilers. However, dietary enzyme supplementation significantly reduced feed intake and improved FCR of broilers as compared to the control. Supplementation of BS4 in liquid or powder form, reduced feed intake by 3.6%. Supplementation of liquid and powder BS4 enzymes improved FCR by 6.4% and 8.9%, respectively, but no different effect between liquid and powder BS4 enzymes on performance of broilers. Nutrient density, enzyme supplementation, and interactions between the two factors did not significantly influence carcass yield, abdominal fat, liver, and gizzard relative weights of broilers.
Co-Authors A. P. Sinurat Arnold P Sinurat Arnold Parlindungan Sinurat B. Listian H Hamid H. Hamid Nurul Pratiwi T. Purwadaria TRESNAWATI PURWADARIA Tresnawati Purwadaria