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All Journal Microbiology Indonesia Journal of Degraded and Mining Lands Management Menara Perkebunan

Happy Widiastuti

Indonesian Research Institute for Biotechnology and Bioindustry

Author-ID : 2840332

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Trenching Construction in Peat Soil and the DGGE Analyses of nif Gene and Activity of Dehydrogenase HAPPY WIDIASTUTI; DARMONO TANIWIRYONO; IMAN RUSMANA; GALUH WENING PERMATASARI
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Basal stem rot (BSR) is a major disease in oil palm crops which also possible happen on peat soils. Ganoderma boninense is the pathogen that causes this disease infects the plants through the root. Maintaining root health expects to reduce the intensity of the occurrence of BSR disease and its spread. In this research, trenching construction and maintenance is carried out which aims to break the spread and activity of Ganoderma. Trenching maintenance is carried out by giving empty bunches oil palm inoculated with decomposer, Azotobacter inoculants and humic acid. Four treatments tested were trenched (T1) and untrenched (T0) in combination with two level of Ganoderma attack, which are moderate (Mo) and severe (Se). The soil sample were retrieved from different point, which are in the trenching area (TR), harvest path zone (H), and frond stack zone (M). The soil characteristic including microbe abundance, dehydrogenase activity, and PCR-DGGE of microbe community in each treatment were analyzed to reveal the effect of trenching construction. The results showed that trenching implementation reveals major results related to the abundance of microbes and fungi population, supported by the enhancement of dehydrogenase activity at the block with moderate Ganoderma attack. In addition, the DGGE study effectively separates the microbial population of four separate treatment into two clusters, splitting the grouping depending on the Ganoderma level attack. This study shows enhancement of soil characteristics biologically and nutrients status of oil palm leaves especially P, as the results of trenching construction in peatland. Keywords: peatland; oil palm; trenching, DGGE; microbes

Exploration of lignocellulolytic microbes in oil palm rhizosphere on peat soils and their respiration activities Happy Widiastuti; Siswanto; Saeful Anwar; Supiandi Sabiham; Husni Mubarok; Darmono Taniwiryono; Basuki Sumawinata; Heru B. Pulunggono
Microbiology Indonesia Vol. 15 No. 1 (2021): March 2021
Publisher : Indonesian Society for microbiology

Microbial respiration in peatlands plays a role in contributing CO2 emissions. Studies of microbial exploration and respiration on peat soils in oil palm plantations have not been widely reported. This study aims to explore lignocellulolytic microbes found in peat soils in compared with mineral soils planted with 12-year-old oil palm. Exploration is done by growing the samples on the specific medium of each group of microbial functions. In the next stage, the culture obtained was analysed the respiration activity based on the oxidation of peroxidase catalysis using a chromogen substrate (tetramethylbenzidine) and measured using spectrophotometry at a wavelength of 450 nm. The results showed that both in mineral and peat planted with oil palm in a depths of 0-20 cm were found lignolytic fungi with a population of 17 x 102. Similar results were also found in peat with fern vegetation but at a depth of 20-40 cm. Lignolytic bacteria (methylene blue degradation) can be found on peat soils planted with oil palm at a depth of 0-60 cm and the population increases with increasing depth. This bacterium is also found on peat soils with fern vegetation and mineral soils planted with palm. At a depth of 0-20 cm the population of lignolytic bacteria in non-oil palm peat is highest. Cellulolytic bacteria were isolated at a depth of 0-60 cm. Cellulolytic bacterial populations were highest in oil palm peat at all depths compared to other samples. Respiration analysis of several dominant isolates showed fairly high variation between microbial function groups and within the same function group. The lignolytic microbial group degrading methylene blue showed high respiration activity and varies greatly (0.19-1.85 MER). While the respiration activity of cellulolytic bacteria ranged from 0.45 to 0.62 MER.

Co-Authors . PRIYONO . SISWANTO Atang Sutandi Basuki Sumawinata Ciptadi Achmad YUSUP Darmono Taniwiryono Darmono Taniwiryono Deddy PURWANTORO Deden Dewantara ERIS Deden Dewantara ERIS Djoko Santoso Djoko Santoso GALUH WENING PERMATASARI Hayati Minarsih Heru B. Pulunggono Heru Bagus Pulunggono Husni Mubarok Husni Mubarok Iman Rusmana Moh Zulfajrin Nizam Tambusai Saeful Anwar Siswanto Siswanto Siswanto Syaiful Anwar Tri Eko WAHYONO Tri Panji

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Journal : Microbiology Indonesia