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Unggul P Juswono
Jurusan Fisika, Fakultas MIPA, Universitas Brawijaya, Malang
Environmental Science

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Analysis of Radiant Gamma Illumination Effects on Pancreatic Insulin Levels Before and After The Administration of Pare Fruit Extract (M. charantia) On Weed Mice Glucose Zulkarnain Zulkarnain; Johan A. E. Noor; Unggul P Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1434.206 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.1

Abstract

Gamma rays are ionizing radiation causes cells damage. Cells damage was caused by radiation related to free radicals as a product when the interaction was happened. The cell defense mechanisms against free radicals involves an active compound called antioxidant that can neutralize it. The objectives of this research were to analyze effects of gamma radiation and to investigate the effects of M. charantia extract giving on the pancreas resistance in producing insulin. One hundred eighty mice were used in this research. They were divided into four group: negatif control (K-), radiation non-extract (R-), positif control (K+) and radiation with extract (R+). The Mice were treated with 200 mg/kg BB, 400 mg/kg BB, 600 mg/kg BB, 800 mg/kg BB, and 1000 mg/kg BB. Exposure of gamma rays is given fractionally for five days with 100 rad per fraction ranging from 100 rad, 200 rad, 300 rad, 400 rad and 500 rad per treatment. Insulin levels was measured by ELISA. The results showed that radiation exposure reduced the insulin levels, increase in radiation dose causes increasing of reduction of insulin levels. The giving of M. charantia extract reduced of reduction of insulin levels and also maintained the insulin levels.
Effects of Turmeric (Curcuma domestica) in Maintaining Reduced Raw Cattle Protein Levels Unggul P Juswono; Johan A E Noor; Avika Dyah Respati
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1390.023 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.15

Abstract

A biological tissue that exposed by gamma radiation is known to be ionized that cause heavily damage due to the presence of free radicals. One of the damage may occured in the protein molecules which cause decreaseing of protein level of the beef so it needs a research on the effect of turmeric yellow (as antioxidant) in the maintaining of the protein level of the beef. In this research, the sample were meats without added turmeric as a control and the other were meats added with turmeric and then irradiated using radioactive elements Cs-137 and Co-60. Protein levels were tested using the standart method to analyze the levels of soluble protein. The results showed that the addition of turmeric had an influence to keep the protein value of beef. This situation suggests that turmeric is an antioxidant that is very good in counteracting free radicals.
Radiation Effects on Decreased Estrogen with Isoflavone Consumption to Prevent Early Menopause in Radiation Therapy Yeni Cahyati; Didik Rahadi Santoso; Unggul P Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1456.776 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.2

Abstract

Radiation effect is one of which is the reduction in estrogen. Gamma rays are ionizing radiation that can lead to cell damage due to free radicals produced when the radiation interacts with cells. The level of cell damage can be minimized by antioxidants. Antioxidants used is the isoflavones of soy milk, so it can be know the effects of radiation on the decline in estrogen that accompanied the consumption of isoflavones to prevent early menopause in radiation therapy.Radiation exposure are given fractionally. The dose was 1,5 Gy in once exposure and given every 3 days up to a maximum dose of the treatment groups. Consumption of soy milk to the mice done orally. Blood was drawn and serum was made. Serum estrogen levels will be tested by using ELISA.The results showed that the ovarium disfunction and stress experienced by mice (Mus musculus) caused by radiation exposure. Maximum results in the consumption of isoflavones on estrogen levels the subject to radiation exposure is 44 mg/day and 131 mg/day of soy milk. This isoflavone dose increase in estrogen levels maximumly. The increase was the response given by the body's immune system of mice due to start reacting and isoflavones are phytoestrogens.
Co-Authors Avika Dyah Respati Didik Rahadi Santoso Johan A E Noor Johan A. E. Noor Yeni Cahyati Zulkarnain Zulkarnain