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Sendow I
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The prevalence of Japanese-B-Encephalitis in different species in Indonesi I, Sendow; Bahri, S; Sarosa, A
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.881 KB) | DOI: 10.14334/jitv.v5i1.178

Abstract

Japanese-B-Encephalitis (JE) is a zoonotic disease which is characterized by encephalitis, caused by JE virus. The situation of this disease has not been known in both animals and human in Indonesia. This paper reports serological finding using competitive - ELISA to evaluate 953 serum samples, comprised of chicken, ducks, cattle, goats, horses, dogs, pigs and human from different areas in Indonesia. The antibody against JE virus was detected in animals and human sera, with prevalence varied among species and location. Cattle showed the highest prevalence of reactor (51 %) while pigs, dogs and horses had the lowest reactor (11%,12% and ]4%). The highest prevalence of reactor in cattle was found in North Sumatera (86%) and the lowest was found in West Java (23%). In goat, the highest prevalence of reactor was found in West Kalimantan (59%) and the lowest was detected in South Sulawesi (14%). Antibody against JE virus was also detected in chicken with the highest prevalence in North Sumatera and West Kalimantan (44%) and the lowest was in South Sulawesi (36%). The highest percentage of reactor in pigs was detected in South Sulawesi (50%) and the lowest was detected in West Kalimantan (2%). In human, the highest prevalence of reactor was found in West Kalimantan (30%) and the lowest was fowld in Irian Jaya. This result provide more information for further research, therefor the JE cases in Indonesia and its social, economic and psychological impacts can be anticipated as earlyas possible.   Key words: Japanese-B-Encephalitis, antibody, animals, human, ELISA
Effectivity of PCR and AGID methods to detect of enzootic bovine leukosis in Indonesia Saepulloh M; Sendow I
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.402 KB) | DOI: 10.14334/jitv.v20i1.1120

Abstract

Enzootic Bovine Leucosis (EBL) is one of viral diseases in cattle caused by bovine leukemia virus (BLV), from Retroviridae. The virus can be detected using severals methods such as Polymerase Chain Reaction (PCR), while antibody can be detected using Agar Gel Immunodifussion (AGID). The aim of this experiment was to study the effectivity of PCR and AGID methods to detect enzootic bovine leukosis virus in Indonesia. Samples of peripheral blood leukocyte (PBL) were collected from cattles those with and without showing clinical signs. A total of 307 blood  and serum samples were  tested against BLV using PCR and AGID tests, while 21 semen samples which were from similar animals for blood collection were collected only for PCR test. The results indicated that twelve cattles have positive  results with PCR test in PBL, but from those cattles  only seven were positive with AGID. On the other hand, the PCR did not detect EBL in 21 bovine semen samples tested, although one sample gave positive result with PCR in PBL. This results indicated that PCR method from blood samples was more sensitive than that AGID method. The PCR detection was also more sensitive for PBL than that for semen samples.
The prevalence of Japanese-B-Encephalitis in different species in Indonesi Sendow I; S Bahri; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 1 (2000): MARCH 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.881 KB) | DOI: 10.14334/jitv.v5i1.178

Abstract

Japanese-B-Encephalitis (JE) is a zoonotic disease which is characterized by encephalitis, caused by JE virus. The situation of this disease has not been known in both animals and human in Indonesia. This paper reports serological finding using competitive - ELISA to evaluate 953 serum samples, comprised of chicken, ducks, cattle, goats, horses, dogs, pigs and human from different areas in Indonesia. The antibody against JE virus was detected in animals and human sera, with prevalence varied among species and location. Cattle showed the highest prevalence of reactor (51 %) while pigs, dogs and horses had the lowest reactor (11%,12% and ]4%). The highest prevalence of reactor in cattle was found in North Sumatera (86%) and the lowest was found in West Java (23%). In goat, the highest prevalence of reactor was found in West Kalimantan (59%) and the lowest was detected in South Sulawesi (14%). Antibody against JE virus was also detected in chicken with the highest prevalence in North Sumatera and West Kalimantan (44%) and the lowest was in South Sulawesi (36%). The highest percentage of reactor in pigs was detected in South Sulawesi (50%) and the lowest was detected in West Kalimantan (2%). In human, the highest prevalence of reactor was found in West Kalimantan (30%) and the lowest was fowld in Irian Jaya. This result provide more information for further research, therefor the JE cases in Indonesia and its social, economic and psychological impacts can be anticipated as earlyas possible.   Key words: Japanese-B-Encephalitis, antibody, animals, human, ELISA
Co-Authors A Sarosa S Bahri S Bahri Saepulloh M