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ANALISA BEBERAPA PARAMETER MOTILITAS SPERMATOZOA PADA BERBAGAI BANGSA SAPI MENGGUNAKAN COMPUTER ASSISTED SEMEN ANALYSIS (CASA) Sarastina Sarastina; T. Susilawati; G. Ciptadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 6, No 2 (2007): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

ABSTRAKTujuan Penelitian ini adalah untuk mempelajari 18 parameter motilitasspermatozoa menggunakan metode CASA serta motilitas progresif secara visualdibandingkan ddengan metoda CASA. Materi adalah semen segar dari 8 bangsa sapiyaitu Limousin, Bali Madura, Simental, Brahman, dan Ongole. Sata dianalisa dengananalisis varian, sementara data motilitas progresif diuji t-test. Hasil menunjukkanbahwa motilitas progresif menggunakan CASA tidak berbeda nyata (P>0.05) diantara6 bangsa sapi. Sementara itu parameter motilitas hiperaktif menggunakan CASA tidakberbeda nyata (P>0.05) kecuali on linier, non limear, juga parameter: DCL, DAP,DSL, VCL, VAP, VSL, LIN< STR< WOB, ALH, AOH adalah berbeda nyata(P<0.05) diantara bangsa sapi. Uji t pada motilitas progresif menunjukkan tidak adabeda nyata (P>0.05) antara CASA dengan visula.Kata kunci: Motilitas, CASA, spermatozoa, sapi.ABSTRACTThe aims of this research were to determine 18 parameters spermatozoamotility using CASA method and to determine spermatozoa progressive motility usingvisual assessment and CASA. The material of this research were bull fresh semenfrom 6 breeds, there were Limmousin, Bali, Madura, Simmental, Brahman andOngole which owned by National Artificial Insemination Center Singosari. Data werecollected from CASA method of 18 parameters sperm motility and visual assessmentmethod of progressive sperm motility. Data were analyzed by variance and factoranalysis, and progressive motility using visual and CASA were analyzed by paired TTest.The result showed that motility and progressive motility using CASA was nosignificant different (P>0.05) for six breeds. There were no significant different(P>0.05) on hyperactive parameter using CASA, but on linear, non linear, curvelinear and cell detail parameter : DCL, DAP, DSL, VCL, VAP, VSL, LIN, STR,WOB, ALH, AOH were significant different (P<0.05) among breeds. Analysis usingpaired T-test of progressive motility assessment was no signifgicant different betweenCASA and visual microscopic (P>0.05).Key words : Motility, spermatozoa, CASA, breeds
ANALISIS ISOENZIM UNTUK MEMPELAJARI VARIASI GENETIK SAPI BALI DI PROVINSI BALI Sri Rahayu; SB Sumitro; T. Susilawati; Soemarno Soemarno
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/390

Abstract

The aim of this research was to know Bali cattle genetic variation according to the band pattern of isoenzyme. Esteraseand Malate dehydrogenase Isoenzymes of Bali cattle were studied. Using the native-PAGE method, the analysis has been made of the genetic structure and variation of the bali cattle population. Isoenzyme isolated from leucocytes cell using homogenation method by adding Phosphat Buffer Saline (PBS). A hundred sample of Bali cattles were taken in Mambang, Slemadeg and Kuwumkeladi. The result of this research indicate that from the 2 different enzyme, 3 loci were detected, and 1 of them was polymorphic (MDH-2). There was null allele phenomenom in MDH-2 locus. The loci polymorphic proportion of three population were 0,333. Chi-Square analysis of three population were 1.251–1.560. The heterozygosity value of three population (Mambang, Slemadeg and Kuwumkeladi) were 0.098, 0.111 and 0.118, respectively.