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All Journal HAYATI Journal of Biosciences BERITA BIOLOGI
Ida Hanarida
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Inheritance of Blast Resistance (Pyricularia grisea Sacc.) on Interspecific Crossing between IR64 and Oryza rufipogon Griff DWINITA WIKAN UTAMI; HAJRIAL ASWIDINNOOR; SUGIONO MOELJOPAWIRO; IDA HANARIDA; REFLINUR REFLINUR
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.406 KB) | DOI: 10.4308/hjb.13.3.107

Abstract

Blast disease affected by Pyricularia grisea causes high percentage of yield losses in rice production. The improvement of durable Blast resistance is difficult due to the complexity of the inheritance of this trait. This study was conducted to evaluate the genetic control and inheritance of Blast resistance trait in interspesific population between IR 64 (accepted Indonesian rice type, medium resistant to Indonesian Blast pathogen) and Oryza rufipogon (AA genome; acc. No.IRGC#105491; donor for Blast resistance). Six populations, i.e. P1, P2, F1, F2, BCP1, and BCP2, were inoculated with three Indonesian races of Blast pathogen. The disease intensity was determined on the basis of disease leaf area (DLA). The three types of gene interactions (additive, dominance, and epistatis) were significantly involved in this trait. Among the digenic epistasis interactions, all of the additive x additive [i], additive x dominance (j) and dominance x dominance (l) contributed to the trait. Broad-sense heritability ranged from 50.30 up to 91.20%, while the narrow heritability ranged from 16.98 up to 73.20%. The presence of additive gene effect indicated that selection of this trait is promising. Key words: inheritance, Blast resistance, wild rice, Oryza rufipogon
SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH Dwinita W Utami; Aderahma Ilhami; Ida Hanarida
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1966

Abstract

Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.
Co-Authors Aderahma Ilhami DWINITA W UTAMI Dwinita Wikan Utami HAJRIAL ASWIDINNOOR REFLINUR REFLINUR SUGIONO MOELJOPAWIRO