Robby Makimian
Departemen Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan Unika Atma Jaya

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EFEKTIVITAS BIOLARVASIDA EKSTRAK DAUN KEMANGI (Ocimum basilicum) TERHADAP LARVA Aedes aegypti Anastasia Christella Christella; Robby Makimian; Rita Dewi
Bahasa Indonesia Vol 19 No 1 (2020): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v19i1.1204

Abstract

Introduction: Indonesia as a tropical country is vulnerable to mosquito-borne diseases like dengue fever, chikungunya, and zika by Aedes aegypti mosquito. Various efforts have been made to eradicate mosquitoes, one of which is by fogging. But this method only eradicates adult mosquitoes and its smoke can also have a bad impact for human health. Therefore, a variety of herbal plants began to be used as biolarvacide, including basil plants (Ocimum basilicum). Methods: This is an experimental research by comparing the lethal effect between concentration and time. In this experiment there were 10 larvas used for each concentration, which were 0,4%, 0,7%, 1,0%, 1,5%, and 2,0%. The lethal effect arising in larval groups is observed every 1 hour in the first 6 hours, and subsequently every 3 hours until 24 hours after exposure. Results: With one way ANOVA test, the result is p=0,012 (p<0,05) this shows that there is a significance difference between the different concentrations and the lethal effect of A.aegypti larvae. With Friedman test, the result is p=0,000 (p<0,05), this shows that there is a significance difference between the different time of exposure of the extract and the lethal effect of A.aegypti larvae. Conclusion: Leaves extract of O.basilicum has lethal effect as biolarvacide against A.aegypti larvae. The higher concentration and the longer time of exposure to O.basilicum extract, increases the lethal effect of A.aegypti larvae.
BIOINSEKTISIDA TERHADAP NYAMUK AEDES AEGYPTI BERBAHAN DASAR EKSTRAK ETANOL DAUN SERAI (CYMBOPOGON CITRATUS) Jennifer Wiranatha; Robby Makimian; Rita Dewi
Bahasa Indonesia Vol 20 No 1 (2021): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v20i1.1222

Abstract

Introduction: Dengue hemorrhagic fever is one of the most common infectious diseases in Indonesia. The vector of this disease is Aedes aegypti (Ae. aegypti). Dengue hemorrhagic fever can be controlled by a few measures, one of them is using insecticide. However, frequent use of chemical insecticide could lead to resistance and is harmful to non-target organisms. One of the solutions for this problem is the use of bioinsecticide derived from lemongrass (Cymbopogon citratus or C. citratus) leaves extract. Methods: The design of this study is true experimental post-test only control group. The population used are adult Ae. aegypti mosquitoes. For each experiment, 10 mosquitoes are required and given the extract of C. citratus in 2%, 10%, and 20% concentrations, and negative control respectively.The lethal effect of the extract is observed in 10, 30, 60 minutes and 6, 12, 18, 24 hours. Results: There is a significant difference in the lethal effect of Ae. aegypti mosquitoes with variations in duration of exposure to the C. citratus leaves extract (p = 0.007), but no significant difference with variations in extract concentration given (p = 0.281). Conclusion: C. citratus leaves extract has bioinsecticidal effect on Ae. aegypti mosquitoes. The optimal result of mosquito mortality is achieved by using the extract in 20% concentration with duration of 12 hours.