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Wa Ode Harlis

Unknown Affiliation

Author-ID : 2865827

Agriculture, Biological Sciences & Forestry Veterinary

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Articles

Kandungan Antioksidan Teh Hijau Daun Mangrove dan Uji Efektifitasnya Sebagai Antikolesterol Pada Mencit Analuddin Analuddin; Andi Septiana; Wa Ode Harlis
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKPenelitian ini bertujuan untuk menentukan senyawa antioksidan teh hijau dan menjelaskan efektifitas teh hijau daun mangrove sebagai anticholesterol pada mencit. Senyawa kimia bahan antioksidaan teh hijau pada daun mangrove Bruguiera gymnorrhiza, B. parviflora, Rhizophora stylosa, R. mucronata, Lumnitzera racemosa, Ceriops tagal dan C. decandra dianalisis dengan GCMS, sedangkan khasiat teh hijau daun mangroves sebagai antikolesterol di ujikan pada mencit. Hasil penelitian menunjukan bahwa senyawa bahan teh hijau bervariasi diantara daun mangrove yaitu polifenol sederhana ditemukan pada daun semua jenis mangrove yang menjadi sampel penelitian dengan konsentrasi yang bervariasi. Senyawa katekin hanya ditemukan pada daun mangrove Ceriops decandra, L. racemosa, R. mucronata dan R. apiculata. Di sisi lain, flavonoid terdeteksi hanya pada daun C. tagal, B. gymnorrhiza dan R. stylosa, sedangkan senyawa T-flavin hanya ditemukan pada daun B. parviflora. Teh hijau daun mangrove mampu menurunkan kadar kolesterol mencit dengan kisaran 33,33 sampai 53,67% mengindikasikan besarnya potensi daun mangrove sebagai bahan teh hijau antikolesterol.Kata kunci: bahan teh hijau, polifenol sederhana, katekin, flavonoid, antikolesterol, daun mangroveABSTRACTThis study aimed to determine the antioxidant properties of green tea material in mangrove leaves and elucidate their capacity on reducing the cholesterol of mice. The chemical properties in leaves of Bruguiera gymnorrhiza, B. parviflora, Rhizophora stylosa, R. mucronata, Lumnitzera racemosa, Ceriops tagal and C. decandra were analyzed by GCMS, while their capability as anticholesterol of mice were examined. The results showed that simple polyphenols were found in all sampled mangrove leaves with different concentration, while the chatechine was found only in leaves of four mangroves including Ceriops decandra, L. racemosa, R. mucronata and R. apiculata. On the other hand, flavonoids was detected only in leaves of C. tagal, B. gymnorrhiza and R. stylosa. Meanwhile, T-flavine was detected only in leaves of B. parviflora. However, green tea material for all of sampled mangroves showed high capacity to reduce cholesterol of mice that ranging from 33.33% to 53.67%, which indicated high potentitiality of mangroves leaves as green tea material of anticholesterol.Keywords: Green tea material, simple polyphenol, cathechin, flavonoid, anticholesterol, Mangroves leaves

Jenis – Jenis Amphibi (Ordo Anura) di Kecamatan Palangga Selatan Kabupaten Konawe Selatan Sulawesi Tenggara Nasarudddin Nasarudddin; Wa Ode Harlis
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 1 (2016): BIodiversitas
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v3i1.1485

ABSTRACT Amphibious existence of the habitat is influenced by several types of habitats eg rice fields, rivers, swamps and secondary forests. South Palangga is a region located in Konsel which has several ecosystems such as forests, savannahs, swamps and mangrove forests. So far the scientific information related to the presence Konawe amphibious species in the South, particularly in the district of South Palangga still very minimal, so it takes a more comprehensive scientific studies related to the presence of wildlife. Destination of the study is to determine the type of amphibious -type (Order: Anura) in the district of South Palangga Privinsi Southeast Sulawesi. The method used is the method VES (Visual Encounter Survey) is a way down both sides of the river and the rice fields and record types of amphibious found. This research was conducted 2 stations 2 stations river habitat and habitat fields. The research result was 606 people composed of 8 types and four families are Bufonidae, Dicroglossidae, Ranidae, and Rhacophoridae.Keywords: Diversity, Amphibian, Habitat, Southern Palangga

PENGARUH PEMBERIAN MONOSODIUM GLUTAMAT TERHADAP STRUKTUR MIKROSKOPIS OVARIUM MENCIT (Mus musculus, L.) Wa Ode Harlis; Sitti Wirdhana Ahmad; Marwati Marwati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 2 (2015): Keragaman dan Bioteknologi
Publisher : University of Halu Oleo

ABSTRACTThis study aims to determine the effect of MSG to the microscopic structure of the ovaries of mice. This study was a experimental study using a completely randomized design (CRD). A total of 16 female mice aged 3 month were devided into 4 groups namely P0 (Control), P1 (0,012 mg/gr BB), P2 (0,015 mg/gr BB) and P3 (0,018 mg/gr BB), MSG administration carried out of 14 days. On day 15 the animals were sacrificed and performed organ harvesting ovaries to make histological preparations. Observation were made by observing the histological structure of the ovaries , and count the number of primary follicles, secondary, tertiary, the korpus luteum and follicular atretic. Data were tested with ANOVA, and LSD (α=0,05%). The result showed the existence of structural damage in the form of a gap between the ovarian follicular granulosa cells and ovarium follicle diameter reduced with increasing doses of MSG given. MSG administration also decreases the average number of follicles in all treatment doses, but the mean of the highest drop in the number of follicles present in treatmen P3 that is, the number of primary follicles (2,50), secondary follicles (2,25), tertiary follicles (1,25) and follicular atretic (4,25), while the corpus luteum is not found.Keywords : Monosodium glutamate, Damaged Structure of The Ovaries, Mice (Mus musculus,L)

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