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All Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan
Andi Magfira Satya Apada
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology Veterinary

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The identification of gram positive bacteria and their effects on kidney histopathology of Amazon Sailfin Catfish (Pterygoplichthys pardalis) in Lapompakka and Sidenreng Lakes, Wajo. Andi Magfirah Satya Apada; Dian Anugrah; Dwi Kesuma Sari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 N0. 2, JULY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i2.19077

Abstract

Pterygoplichthys spp or commonly referred as Pleco is originated from Amazon River in South American Continent.  The aim of this study is to isolate and identify the pathogenic gram-positive bacteria in pleco's kidney (Pterygoplichthys pardalis) and to observe the pleco's kidney histopathology colonized by gram-positive pathogenic bacteria in Sidenreng and Lapompakka Lakes. The samples employed in this experiment includes 10 plecos with each 5 samples represented each lake. The isolation and identification of positive gram bacteria were performed through bacterial culture produced from pleco's kidney swab test on blood agar using gram staining followed by biochemical testing with Vitek 2 compact system. From the results, there were 4 gram-positive bacteria successfully isolated, one of them was Enterococcus faecalis which is the suspected pathogen. Specimen preparation for kidney histopathology was carried out by 10% neutral buffered formalin (NBF) fixation, graded ethanol series dehydration, paraffin wax embedding, 4 μm sectioning, Hematoxylin and Eosin Staining and after that, the specimen was observed. Data analysis employed qualitative descriptive method. From the observation, damages such as necrosis, inflammatory cell infiltration, hemorrhage, and hypertrophy were identified from the pleco's kidney. Kidney damages were most probably caused by the infection by gram-positive pathogenic bacteria Enterococcus faecalis. Keywords : Sidenreng Lake, Lapompakka Lake, Kidney, Histopathology, Plecos.  
The The identification of gram-negative bacteria and their effects on liver histopathology of Amazon Sailfin Catfish (Pterygoplichthys pardalis) in Lapompakka and Sidenreng Lakes, Wajo. Hairul Afzan; Andi Magfirah Satya Apada; Andi Rianti Rhasinta; Wa Ode Santa Monica
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 N0. 2, JULY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i2.19105

Abstract

Amazon Sailfin Catfish (Pterygoplicththys pardalis) or commonly known as Plecos is originated from Amazon River in South America Continent. The aims of this study are to identify and isolate pathogenic gram-negative bacteria in the liver of the plecos from Lapompakka and Sidenreng Lakes and to observe the histopathological changes in liver identified with gram-negative bacteria infection. 10 samples were collected from both lakes with each 5 representing each lake. Identification and Isolation of gram-negative bacteria was performed through culture of pleco's liver swab results in MacConkey media using gram staining method and were continued by biochemical testing using VITEK 2 System. The acquired results indicated that there were 8 different species of gram-negative bacteria isolated and all of them were pathogenic to the plecos. The species of bacteria includes Klebsiella pneumoniae, E.coli, proteus sp., Acinetobacter baumannii, Aeromonas sobria, Enterobacterb cloacae complex, Citrobacter freundii, Enterobacter aerogenes. Specimen preparation for liver histopathology was carried out by 10% neutral buffered formalin (NBF) fixation, graded ethanol series dehydration, paraffin wax embedding, 4 μm sectioning, Hematoxylin and Eosin Staining. After that, the specimen was observed under a microscope. Data analysis employed qualitative descriptive method. From the observation, liver damages such as necrosis, inflammatory cell infiltration, hemorrhage, and cell hyperthrophy were identified. Liver damages were most probably caused by the infection of gram-negative bacteria. Keywords : Sidenreng Lake, Lapompakka Lake, Liver, Histopathology, Amazon Sailfin Catfish
EFEK ADAPTOGENIK EKSTRAK ETANOL KULIT BUAH MANGGIS (Garcinia mangostana L.) PADA MENCIT (Mus musculus) MENGGUNAKAN METODE SWIMMING ENDURANCE TEST Abdul Wahid Jamaluddin; A. Magfira Satya Apada
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 1 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.796 KB) | DOI: 10.36387/jiis.v5i1.385

Abstract

Adaptogenic activity was related to antioxidant activity which can reduce the number of free radicals, which in turn will reduce stress and improve body balance. Mangosteen rind (Garcinia mangostana L.) with a large antioxidant content promises to be Adaptogen. This study aims to examine the Adaptogenic effects of Ethanol Extract of mangosteen peel (Garcinia Mangostana L.) Using the Swimming Endurance Test (SET) in Mice. Simplicia of Mangosteen Fruit extracted by maceration using 96% ethanol, then tested its Adaptogen activity based on swimming endurance test. The group consisted of negative control (NaCMC 1%), positive control (Vit C. 100 mg / kg BB) and 3 groups treatment of ethanol extract of mangosteen peel ranging from 100, 200 and 400 mg / kg BB where all groups were given oral 1x1 oral administration for 7 days and on the 8th day, a SET test were performed. After that, data analysis was performed statistically. The results showed that the ethanol extract of mangosteen peel had the ability to be Adaptogenic where mice swimming in all groups given mangosteen peel extract was longer than the negative group with a significant difference (p <0.05). Ethanol extract of mangosteen peel showed a strong adaptogenic antistress effect in mice based on increased swimming time ability of mice.
Identification of Yersinia spp in Catfish (Claria spp) Attacked Enteric Redmouth Disease at the Education Veterinary Clinic, Hasanuddin University Fedri Rell; Dwi Kesuma Sari; Muhammad Fadhlullah Mursalim; Baso Yusuf; Andi Magfira Satya Apada; Astri Caturutami Sjahid
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.21091

Abstract

Catfish is one of the fish that has a high economic value so that it becomes one of the export commodities to meet the consumption and protein needs of the community so that it is widely cultivated. In cultivation, catfish can be attacked by various diseases, one of which can be caused by bacteria. The bacteria that often cause death in fish, both marine and freshwater fish, is Yersinia spp. Yersinia spp is a group of gram-negative bacteria, in the form of bacilli and motile and is also reported to be one of the pathogenic diseases that often attack fish. samples were taken on August 12, 2021 from the mouth organs of catfish at the Hasanuddin University Education Animal Clinic. Samples were cultured in Tryptic Soy Agar (TSA) for 24 hours and then purified in Mac Conkey Agar (MCA). The culture results showed the presence of bacterial growth. Follow-up examination using a microscope and showed the presence of red (Gram negative) and bacilli-shaped bacteria.
Co-Authors Abdul Wahid Jamaluddin Andi Rianti Rhasinta Astri Caturutami Sjahid Baso Yusuf Dian Anugrah Dwi Kesuma Sari Fedri Rell Hairul Afzan Muhammad Fadhlullah Mursalim Wa Ode Santa Monica