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All Journal Hemera Zoa Media Veteriner Jurnal Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Pengabdian Masyarakat Medika
Dwi Kesuma Sari
Study Program Of Veterinary Medicine, Faculty Of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, South Sulawesi, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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The identification of gram positive bacteria and their effects on kidney histopathology of Amazon Sailfin Catfish (Pterygoplichthys pardalis) in Lapompakka and Sidenreng Lakes, Wajo. Andi Magfirah Satya Apada; Dian Anugrah; Dwi Kesuma Sari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 N0. 2, JULY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i2.19077

Abstract

Pterygoplichthys spp or commonly referred as Pleco is originated from Amazon River in South American Continent.  The aim of this study is to isolate and identify the pathogenic gram-positive bacteria in pleco's kidney (Pterygoplichthys pardalis) and to observe the pleco's kidney histopathology colonized by gram-positive pathogenic bacteria in Sidenreng and Lapompakka Lakes. The samples employed in this experiment includes 10 plecos with each 5 samples represented each lake. The isolation and identification of positive gram bacteria were performed through bacterial culture produced from pleco's kidney swab test on blood agar using gram staining followed by biochemical testing with Vitek 2 compact system. From the results, there were 4 gram-positive bacteria successfully isolated, one of them was Enterococcus faecalis which is the suspected pathogen. Specimen preparation for kidney histopathology was carried out by 10% neutral buffered formalin (NBF) fixation, graded ethanol series dehydration, paraffin wax embedding, 4 μm sectioning, Hematoxylin and Eosin Staining and after that, the specimen was observed. Data analysis employed qualitative descriptive method. From the observation, damages such as necrosis, inflammatory cell infiltration, hemorrhage, and hypertrophy were identified from the pleco's kidney. Kidney damages were most probably caused by the infection by gram-positive pathogenic bacteria Enterococcus faecalis. Keywords : Sidenreng Lake, Lapompakka Lake, Kidney, Histopathology, Plecos.  
PF-26 The Use of Endemic Sulawesi Medaka Fish (Oryzias celebensis) as an animal model candidate Dwi Kesuma Sari; Irma Andriani; Khusnul Yakin; Magfira Satya
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

        Medaka fish (Oryzias sp) or also known as "rice fish" are non-consumption fish or ornamental fish that are in great demand by humans. Medaka fish is an animal model that is very well known and has been widely used by researchers in the world for studies in various fields of science, especially biology and medicine, as well as Zebras which have been developed as model animals. Medaka fish also used as animal models for testing Parkinson's disease caused by environmental pollution and genetic factors. Several types of mutant medaka fish have also been made for screening diabetes, cancer and other degenerative diseases.                    From the exploration results, the researchers showed that Sulawesi has a high endemism especially for the family Adrianichthyidae. The high endemism is not only supported by zoogeographic boundaries as islands located on the Weber line and Wellacea lines, but also because of this fish that are traded so that the chance of migrating this fish is small. These facts underlie the statements of several researchers that fish medaka can reveal the mystery of the evolution of aquatic fauna in Sulawesi.                    As an effort to develop Sulawesi Medaka fish (Oryzias celebensis) as a model animal, studies have been conducted with various histology studies with various staining methods in Sulawesi medaka fish so that Hasanuddin University is expected to be a gateway for researchers to obtain information about Medaka fish in the Sulawesi islands and as a center for learning fish medaka.
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells I Ketut Mudite Adnyane; Savitri Novelina; Dwi Kesuma Sari; Tutik Wresdiyati; Srihadi Agungpriyono
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
AH-41 Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate Muhammad Syukur Hamdan; Novi Susanty; I Ketut Mudite Adnyane; Dwi Kesuma Sari
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate
Ultrastructural and Immunohistochemical Studies of Transplanted Canine Lung Carcinoma Cell to Severe Combined Immunodeficiency Mice (STUDI ULTRASTRUKTUR DAN IMUNOHISTOKIMIA TRANSPLANTASI SEL KANKER PARU-PARU ANJING PADA MENCIT SEVERE COMBINED IMMUNODEFF Dwi Kesuma Sari; Bambang Pontjo Priosoeryanto; Dewi Ratih Agungpriyono; Ryoji Yamaguchi; Kazuyuki Uchida; Susumu Tateyama
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Primary lung cancers, or tumors originating in the lung, are relatively uncommon in dogs. The objectiveof this study was to describe the canine lung carcinoma that serially transplanted into severe combinedimmunodeficiency (SCID) mice, in order to established cell line from this tumor cell.  Morphology andcharacteristic of this canine lung carcinoma in SCID mice by histopathological and ultrastructuralexaminations with metastatic lesion in lung were also examined.  Histopathologically, the tumor masswere consisted of cuboidal to columnar cells with papillary pattern, uniform in size, the nuclei were oftenvariable in size, and some cells have vacuole on their cytoplasms.  Glandular forms were predominant withlobulated pattern, ductal pattern with papillary injected into tube-like structure were also encountered.Mitotic figures commonly found with inflammatory reaction were sometimes present in the interstitiumand lumen gland.  Ultrastructural analysis of the tumor cells showed round to oval cells with one or moreprominent nucleoli.  The cells possessed numerous mitochondria, smooth endoplasmic reticulum, andindividual cells which were interconnected via desmosomes.  Tonofilament characterize by long cytoplasmicmaterial was encountered.  Positive reaction of the round to oval tumor cells to anti keratin antibodyconfirmed that their epithelial cell nature.  Lung metastatic lesions were found in SCID mice aftertransplantation and this phenomenon indicated that canine lung carcinoma is tumorigenic to SCID mice.
Histopathologic features of male domestic cat (Felis domestica) testes induced by bisphenol-A orally Fachira Ulfa Makmur; Fika Yuliza Purba; Dwi Kesuma Sari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 2 NO. 1, JANUARY 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v2i1.4341

Abstract

Infertility in cat becomes a serious problem in pet community since many compounds of infertility-caused can be found widely in the environment. One of the compounds causing infertility in mammals is Bisphenol-A (BPA), which is known as an estrogenic compound. The purpose of this research is to observe the changes in testicular histology of male domestic cat (Felis domestica) induced by BPA orally. Sample used in this study were 24 male domestic cats divided into 4 groups, namely the group 1 as control, group 2, 3 and 4 were treated with BPA about 5, 10 and 100 mg/kg bodyweight, respectively for 5 days. The results showed that the estrogenic effect of BPA affects the reproductive system of the cat. The effects of BPA in cat included decrease in the amounts of spermatozoa, vacuolization of seminiferous tubules cells, degeneration of Leydig cells, degeneration of tubular epithelial cells,  irregular forms of the germ, interstitial tissue hemorrhage, debris cell filled the lumen of seminiferous tubules. These results suggested that BPA can be harmful to the reproductive system of the cat
Effects of Kapok Seed Extract(Ceiba pentandra Gaertn) as Contraceptive Agent to the Quality of the Spermatozoa in Domestic Cat (Felis domestica) Sitti Mughniati; Dwi Kesuma Sari; Dedy Rendrawan; Lellah Rahim
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 2 NO. 1, JANUARY 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v2i1.4370

Abstract

Contraception is a way to prevent the fusion of mature egg cell and sperm during the intercourse, so the fertilization will not occur. Some contraceptive methods in small animals have been evolved. This research aimed to determine the effects of kapok seed (Ceiba pentandra Gaertn) extract as contraception agent to the quality of spermatozoain domestic cats. The animals were divided into four group with each group consisted of six male cats. Group I, II, III was designated as the treatment groups and IV as the control group. The cats were given the emulsion of kapok seeds extract orally with concentration of 0,1%  b/v, 0,71 mg/kg BB, 0,36 mg/kg BB and 0,21 mg/kg BB doses. This study used Randomized Complete Random Design (RAL) design from sperm quality data and used ANOVA statistic test. The result showed that the concentration of sperm decreased in the treatment groups compared with the control group. Statistically, the sperm concentration decreased significantly (P < 0,05) between the control group and treatment groups. The average motility in low and medium doses was above 40% while it was below than 40% in the high doses group. Based on the results, the granting of kapok seed extract inhibited the viability of spermatozoa of cats with significant results (P < 0,05). Sperm abnormalities in this study was obtained from the control group with percentage average of 1 % , while it was significantly increased in the treatment groups marked by abnormalities in the shape of the head and tail of the spermatozoa. These results suggested that kapok seed extract can be used as one of the contraceptive agents in domestic cat (Felis domestica) because it can decrease the sperm quality significantly.
The Analysis of Renal Anatomy and Histological Changes of Green Iguana (Iguana Iguana) After Feeding With Red Spinach (Amaranthus tricolor L.) Annita Vury Nurjunitar; Fika Yuliza Purba; Wahyuni Wahyuni; Ismartoyo Ismartoyo; Dwi Kesuma Sari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 1, JANUARY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i1.5816

Abstract

Background and Objective: Iguana breeders usually used red spinach as a feed for iguanas. However, several studies have indicated that red spinach contains oxalate and purine which are harmful if consumed at a certain level because they can interfere with kidney function. This study aimed to investigate the change of anatomy and histology of green iguana (Iguana iguana) kidney after feeding with red spinach. Materials and methods: Twelve iguanas were used in this study and were divided into four groups in accordance to the dose level of red spinach (100%, 75%, 50% and 25%, namely group I to IV, respectively). The red spinach was given for 30 days through the nasogastric intubation. The effect of red spinach administration was observed through the anatomy and histological changes. The results were statistically analyzed with One Way ANOVA with p<0.05 was considered significant, and Post Hoc Test. The histopathology change of the kidney was analyzed descriptively. Results: The administration of red spinach in iguanas caused some anatomy changes of the kidney; such as the enlargement of iguana’s kidney. The histological findings included the enlargement of the glomerulus, capsular space constriction, hydropic degeneration, tubular dilation, necrosis, and formation of connective tissue (fibrosis), uric acid crystal sediment (gout), oxalate crystal, and lymphocyte infiltration. Among four different dose levels of red spinach, 100% of red spinach caused the highest damage to the iguana’s kidney. The significant change grew as the increasing dose of red spinach that was given to the green iguanas’ bodies. Conclusion:  Red spinach caused changes in anatomy structure as well as kidney histology of green iguanas. Severe damages occurred in the treatment group III, moderate damage occurredin the treatment group II, and light damage occurred in the treatment group I. Keywords: Green iguana, Red spinach, Kidney, Anatomy Change, Histology Change
Combination Effect of Golden Snail Powder and Fermented Coconut Cake on Quality of Duck Eggs Nurmauliah Syaharuddin; Rasdyanah Rasdyanah; Dwi Kesuma Sari; Waode Santa Monica; Abdul Wahid Jamaluddin
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 2, JULY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i2.6627

Abstract

This study aims to determinate the effect of combination golden snail powder and fermented coconut cake on quality of duck eggs. We were using experimental feeds combination form golden snail powder and fermented coconut cake as raw material for livestock rearing for duck. This research was conducted in July - September 2018 in Mangkoso Village, Barru Regency and continued with sample testing at the Laboratory Animal Husbandry Faculty of Livestock Product Technology University of Hasanuddin. This study used 16 female duck who are 20 weeks old with four types of treatments consists of four replications of each treatment. The egg quality was carried out using a sample of 16 eggs from Ducks which was maintained and treated with different rations. The data obtained were analyzed using variance analysis with a Completely Randomized test for significant different results. The results of the combination of golden snail powder and fermented coconut cake on egg color, egg white index, egg yolk index, haugh unit (HU) value of egg units. All treatments (P1, P2, P3) gave effect to three parameters, namely eggshell quality, egg yolk index and egg yolk color, while the other three parameters namely egg weight, egg white index and Haugh Unit (HU) value did not differ significantly from negative controls. The best treatment was shown by treatment P3 which gave an effect on the heavier egg weight than other treatments, eggshell quality grade A, Egg White Index and Egg Yolk Index showed good quality and bright golden egg yolks. 
Antibiotic Resistance of E. Coli Isolates from Broiler Chick's Cecum in Makassar City Erdi Purwanto; Dini Marmansari; Dwi Kesuma Sari; Muhammad Hatta
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 2, JULY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i2.7462

Abstract

Broiler chickens or broilers are the cheapest source of animal protein that can be obtained apart from eggs. The high demand for broiler chicken meat is fulfilled because modern broilers grow rapidly and are maintained massively and intensively. Massive maintenance systems make the spread of disease easier. Treatment of bacterial diseases using inappropriate antibiotics can lead to antibiotic resistance. This study aims to look at the antibiotic resistance of Escherichia coli (E. coli) bacteria from broiler chickens. E coli bacteria were isolated from 34 samples of broiler chicken cecum taken from several large traders in the city of Makassar. The E. coli isolates were then tested using Kirby-Bauer's diffuse disc method on 5 types of antibiotics from 5 classes of antibiotics namely: Ampicillin (β-lactam); Tetracycline (Tetracycline); Gentamicin (Aminoglycosides); Enrofloxacin (Flouroquinolone); Sulfamethoxazole-Trimethroprim (Sulfonamide). The inhibition zone formed was interpreted using the CLSI 2018 standard. The results of this study were 79.4% of the samples resistant to Ampisillin, 85.3% resistant to Tetracycline, 61.8% resistant to Sulfamethoxazole-Trimethroprim, 76.5% resistant to Enrofloxacin and 52.9% were resistant to Gentamicin. With 91.18% of the sample being resistant to at least 2 types of antibiotics.
Co-Authors A. Magfira Satya Apada A. MUH. ICHLASUL AKMAL Abdul Halim, Musdalifah Abdul Wahid Jamaluddin Adryani Ris Adyatma Muhammad Nur Alni, Nurul Istiana Amriani, Rini Andi Magfira Satya Apada Andini, Nurul Sulfi Annita Vury Nurjunitar Aras, Irwin Arif, Makruf Arsyad, M Aryadi Astri Caturutami Sjahid Bambang Pontjo Priosoeryanto Baso Yusuf Cangara, Husni Dedy Rendrawan Dewi Mutisari Dewi Ratih Agungpriyono Dian Anugrah Dian Fatmawati DIDIK IMAM SAKIRIN Dini Marmansari Djabir, Yulia Yusrini Erdi Purwanto Fachira Ulfa Makmur Fatimah Mappanyompa Fatmawati, Dian Fedri Rell Ferra Indrawati Fika Yuliza Purba Hamria Pratiwi Hartarto Akhmad Hendra Wibawa Ilham Akbar Rahman Irfan Idris Irfan Idris Irma Andriani Ismartoyo (Ismartoyo) Kazuyuki Uchida Ketut Adnyane Mudite Khusnul Yakin Kusumaningrum, Arsyinta Retno Lellah Rahim Magfira Satya Mufidatul Asmi Muflihanah Muh. Nasrum Massi MUHAMMAD DIRGA GIFARDI Muhammad Fadhil Shalih Muhammad Fadhlullah Mursalim Muhammad Hatta Muhammad Muflih Nur Muhammad Syukur Hamdan Muhammad Zulfadhillah Sinusi Musdalifah Natsir, Sartini Novi Susanty Nurlaila Fitriani Nurmauliah Syaharuddin Purba, Fika Yuliza Rarasmika, Ainun Rasdiyanah Rasdyanah Rasdyanah Ratna Ryoji Yamaguchi Sartini Natsir Savitri Novelina Sitti Mughniati Sri Deniati Srihadi Agungpriyono Suanti Supri Susumu Tateyama Tutik Wresdiyati Wahyuni Wahyuni WAODE SANTA MONICA Yulia Yusrini Djabir