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A. Indrianto
Faculty of Biology, University of Gadjah Mada, Jl. Teknika Selatan, Sekip Utara Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Peningkatan Pembentukan Embrio Somatik pada Wortel (Daucus carota L) Menggunakan N6-benzylaminopurine (BAP) ., Rusdianto; Indrianto, A.
bionature Vol 16, No 2 (2015): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.982 KB) | DOI: 10.35580/bionature.v16i2.2462

Abstract

Salah satu proses pembentukan planlet dalam teknik kultur jaringan tumbuhan adalah embriogenesis somatik, yaitu suatu proses pembentukan embrio dari eksplan yang berupa sel-sel somatik yang telah mengalami dediferensiasi. Penelitian ini bertujuan meningkatkan pembentukan embrio somatik pada waortel (Daucus carota L) menggunakan BAP. Penelitian ini dilakukan dalam 3 tahap yaitu; (1) Tahap perkecambahan  in-vitro, menggunakan medium ¼ MS; (2) Tahap induksi dan pemeliharaan kalus, menggunakan medium MS + 2,4-D 2 mg/l ; (3) Tahap induksi embrio somatik. Hasil penelitian menunjukkan biji wortel dapat berkecambah dengan baik pada medium ¼ MS dengan rata-rata persentase perkecambahan mencapai 98 % dan panjang hipokotil 3,84 cm. Efisiensi pembentukan kalus mencapai 90.83 %, Warna kalus umumnya putih bening atau putih kekuningan dengan tekstur friable atau remah. Perlakuan 0,5 mg/l BAP merupakan konsentrasi yang paling tepat meningkatkan pembentukan embrio somatik, dengan rata-rata jumlah embrio fase globuler 54,00; fase jantung 5,33; dan fase torpedo 4,66, dibandingkan dengan konsentrasi perlakuan BAP yang lain.
MORPHOLOGICAL CHARACTERISTICS, PRODUCTIVITY AND QUALITY OF THREE NAPIER GRASS (Pennisetum purpureum Schum) CULTIVARS HARVESTED AT DIFFERENT AGE Budiman, B.; Soetrisno, R.D.; Budhi, S.P.S.; Indrianto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 4 (2012): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.4.294-301

Abstract

The research was conducted to determine the morphological characteristics, productivity andquality of Napier grass cultivars Taiwan, King and Mott harvested at 8 and 13 after planting. Effect ofcultivar and harvest arranged in a nested design (3x2). The variables were observed consisting ofmorphological characteristics, dry matter production and forage quality. The results showed that themorphological characteristics, leaf stem ratio, fiber content, crude protein content and in vitro dry matterdigestibility of Napier grass Taiwan and King cultivar were not different, but both morphologicalcharacteristics, fiber content was higher (P <0.05) compared to Mott cultivar, whereas leaf stem ratio,crude protein content and in vitro dry matter digestibility Mott cultivar was higher (P <0.05) comparedto Taiwan and King cultivars. In general, an increase in plant height and fiber content at the age of 13weeks, whereas crude protein content and in vitro dry matter digestibility decreased. It was concludedthat the highest dry matter production found in Taiwan and the King cultivars, whereas highest qualityfound in Mott cultivar. Dry matter production was highest harvested at 13 weeks, whereas the highestquality harvested at 8 weeks after planting for all cultivars tested.
TOTAL NON-STRUCTURAL CARBOHYDRATE (TNC) OF THREE CULTIVARS OF NAPIER GRASS (Pennisetum purpureum) AT VEGETATIVE AND REPRODUCTIVE PHASE Budiman, B.; Soetrisno, R.D.; Budhi, S.P.S.; Indrianto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.126-130

Abstract

An experiment was conducted to determine Total Non-structural carbohydrates (TNC) of threecultivars of napier grass (Pennisetum purpureum) harvested at vegetative and reproductive phases. Thecultivars tested were Taiwan (Gt), King (Gk) and Mott (Gm) and arranged in a 3 x 2 of treatments withfour replicates following nested design. The results showed that the highest sugar content (P<0.01) wasfound in Gt cultivar and the lowest was in Gm cultivar. The highest starch content (P<0.01) was found inGk cultivar and the lowest was in Gt cultivar. TNC content of Gt and Gk cultivars were not significantlydifferent, but both were significantly higher (P<0.01) compared with the Gm cultivar. It can beconcluded, that there were differences in TNC between cultivars, however, the TNC content in Gkcultivar was not different with Gt cultivar, while Gm cultivar have the lowest (P<0.01) TNC content. Atreproductive phase all cultivars have higher (P<0.01) TNC and starch content than at vegetative phase
Co-Authors B. Budiman R.D. Soetrisno RUSDIANTO ., RUSDIANTO S.P.S. Budhi