Muhammad Ja’far Luthfi
Sunan Kalijaga State Islamic University Yogyakarta

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A Simple and Practical Method for Rat Epididymal Sperm Count (Rattus norvegicus) Muhammad Ja’far Luthfi
Biology, Medicine, & Natural Product Chemistry Vol 4, No 1 (2015)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.743 KB) | DOI: 10.14421/biomedich.2015.41.1-3

Abstract

Sperm sample from epididymal source can be determined its number using minimal amount of equipment. These method will aid researcher and practitioner in sperm quality analysis to determined sperm number rapidly and practically.
Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton Muhammad Ja’far Luthfi
Biology, Medicine, & Natural Product Chemistry Vol 5, No 1 (2016)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1068.409 KB) | DOI: 10.14421/biomedich.2016.51.19-22

Abstract

Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.
Morpho-Anatomical Analysis of Cosmostigma racemosum (Asclepiadoideae) Flowers Widodo Widodo; Mohamad Amin; Mimien Henie Irawati Al-Muhdar; Muhammad Ja’far Luthfi
Biology, Medicine, & Natural Product Chemistry Vol 3, No 1 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1600.439 KB) | DOI: 10.14421/biomedich.2014.31.35-46

Abstract

Cosmostigma racemosum is a plant species belonging to the family Apocynaceae, the subfamily Asclepiadoideae. Cosmostigma racemosum is found in Nglanggeran Mountain Gunungkidul, Yogyakarta. The local name and its original distribution are not known. Information or study of Cosmostigma racemosum in Indonesia is not available. Comprehensive characterization of this species is important for authentication and addition of data base. Characterization was conducted by analyzing the morphology and anatomy of flower. The objectives of this study were to describe and analyze the morphology and anatomy of C. racemosum flowers.  The method of research was based on observation method and exploration of plant systematics evidence or taxonomy evidence, including analyses and description of morphology and anatomy of flower structure and its development.  The results showed that the characteristics of flower morphology are in accordance with the existing description in literatures. Characteristics of pollinia are specific characters of morphological aspect of flower. Data of anatomy of flower and its parts development are the new ones which confirm the position of C.racemosum as a member of the tribe Marsdenieae. The data of anatomy also show new information of the ontogeny of the important parts of flower: pollinia formation, pollinia corpusculum, anther wall, anther sac, stigma, stamen, staminal tube, stigmatic chamber, and structure of ovary in Asclepiadoideae
Effect of Lunasia amara Blanco on Sperm Number, Sperm Motility, and Testicular Histology of Male Rats Muhammad Ja’far Luthfi
Biology, Medicine, & Natural Product Chemistry Vol 4, No 2 (2015)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.029 KB) | DOI: 10.14421/biomedich.2015.42.31-33

Abstract

Sanrego (Lunasia amara), has been used in the folk medicine to increase and/or to treat male fertility. However there is no scientific evidence to confirm the positive effect of the plant on an improvement of male fertility. The objective of this research was to study the effects of the plant (on adult Sprague-Dawley male rats) at the doses of 30 mg/kg, 60 mg/kg, and 90 mg/kg on the sperm count, motility, and testicular histology. Administration were given by force-feeding between 10.00 am and 12.00 pm daily for a period of 42 days followed by sperm quality analysis and testicular histology evaluation. The sperm analysis showed that the sanrego increased the sperm count and sperm motility. The testicular histology also revealed positive effect of the plant on spermatogenesis.   Overall the present study showed the sanrego is potential plant to increase male fertility.
Medicinal Plants: A Prospect in Developing Male Fertility Enhancing Agent Muhammad Ja’far Luthfi; Mahanem Mat Noor; Jalifah Latip
Biology, Medicine, & Natural Product Chemistry Vol 4, No 2 (2015)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.601 KB) | DOI: 10.14421/biomedich.2015.42.41-47

Abstract

Medicinal plants have been a revolutionary breakthrough in the treatment of male sexual dysfunction. Traditional medicine based on a holistic philosophy is quite different with the practice of “western” medicine. Phytochemical substances focus their mechanisms of healing to the root of cause, i.e. the inability of controlling the proper function of the whole body system. Hence, medicinal plants manage sexual dysfunction and male fertility in the frame of sexual dysfunction as a whole entity. Some previous researches prove that the use of medicinal plants have a good impact in the treatment of a variety of male sexual problems. This paper will discuss several important aspects of aphrodisiac plants and preliminary study regarding them in Indonesia. Difficulties inherent to activity guided isolation and the specific requirements of bioassays are also discussed.