Garuda - Garba Rujukan Digital

Muhammad Marzuqi

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Author-ID : 2890527

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

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NUTRIENT DIGESTIBILITY FEED WITH DIFFERENT LEVELS OF PROTEIN AND LIPID ON CORAL ROCK GROUPER (Epinephelus corallicola) JUVENILE Muhammad Marzuqi; Dewi Nasbha Anjusary
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The aims of this study was to determine the nutrient digestibility coefficient of diet with diertary levels of protein and lipid on coral rock grouper (Epinephelus corallicola) juvenile. This study used completely randomized design with factorial arranged in 3 different treatment of dietary protein levels i.e., 36%, 42%, and 48%;and 2 different treatment of dietary lipid levels i.e., 9% and 18%; with 3 repetition. The average initial body weight of fishes for this study was 29.19 ± 0.97 g/fish. Fishes were reared in 18 pieces of polycarbonate tank with water volume of 30 liters with stocked density of 7 fishes/tank. The tank equipped with aeration with water flow change of 20 liters/hour. Fish were fed 2 times per day on at satiation and fish rearing for 150 days. Parameters measured were protein, lipid, carbohydrate and energy of digestibility coefficient . The results showed that the interaction of protein and lipid levels that differ in feed ratio significantly affected on protein, lipid, carbohydrate, and energy digestibility coefficients. In general, feed with a protein content of 36% and 9% lipid produced good nutrient digestibility coefficient and better economic value. Keywords: protein, lipid, feed nutrient digestibility, coral rock grouper

PERIODS OF MOUTH OPENING AND YOLK ABSORPTION RELATED TO THE ACTIVITY OF DIGESTIVE ENZYMES IN THE EARLY STADIA FROM THE SECOND GENERATION OF HUMPBACK GROUPER BROODSTOCK Wawan Andriyanto; Muhammad Marzuqi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 4 No. 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The experiment was carried out to determine the exposure time of the first mouth opening on humpback grouper larval from F2 broodstock on hatchery until 10 DAH (days after hatching) because this phase is a critical phase of larval adaptation from food endogenous (from yolk sac) to the ability of absorbing nutrients from external (exogenous). The eggs used were collected from natural spawning of second generation of humpback grouper reared in the tank size of 150 litre. The mouth opening was observed at 90 and 45 degrees under computerize integrated microscope and processed by a specific software. The rate of yolk absorption was observed in larvae from the beginning to the end of breeding, while the activities of digestive enzymes (trypsin and chymotripsin) were analyzed by enzyme assay techniques. The results showed that the period of mouth opening of larvae of humpback grouper occurred at 3 DAH with mouth openings of 45° as large as 0.103 mm and of 90° as large as 0.156 mm. In the early stadia, the diameter of yolk was 0.1875 mm, while the oil globule was 0.0537 mm. At 4 days after hatching, the yolk and oil globule had been absorbed, while the enzymes trypsin and chymotripsin were detected in the early stadia. The results also showed that the larvae was started to use foods from outside after the age of 4 DAH. Up to the age of 10 days, the mouth opening increased and the activity of trypsin and chymotripsin were still detected.Keywords: progeny larvae of humpback grouper F2, mouth opening, yolk sac, enzyme

EFFECT ON DIETARY PROTEIN AND FEEDING RATE ON GROWTH OF TIGER GROUPER (Epinephelus fuscoguttatus) JUVENILE Muhammad Marzuqi; Ni Wayan Widya Astuti; Ketut Suwirya
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 4 No. 1 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The grouper fish culture was developed after its succesful seed production in hatchery well known. In grow-out culture grouper, the protein requirement and feeding rate have to know well in order to understand the effectiveness on feed utilization . The experiment was designed by factorial design with the first factor as 3 dietary protein (36%, 42%, 48%) and the second factor as 3 feeding rate ( 1,5%, 2,0%, 2,5%). Ten of juvenile tiger grouper (102, 51-102, 73 g of body weight) were stocked in 27 polycarbonate tanks with 400 l volume each. Each tank was equiped with aeration and flow-through water system. Fish was fed three time per day i.e. 08:00, 11:30 and 15:30 Central Indonesia Standard Time for 16 weeks. Every week was measured body weight, survival rate, and feed quantity. The parameter used in this experiment were weight gain, spesific growth rate, feed efficiency and survival rate. The results of the experiment showed that the combination of protein level (36%, 42%, 48%) with feed ratio (1.5%, 2.0%, 2.5%) gave response on growth, weight gain and survival rate. Combination dietary protein level of 48% and feed ratio 1.5% gave higher weight gain and survival rate with values of 104.05 ± 5.89% and 96.67 ± 5.57 %, respectively.Keywords: Dietary protein and feeding rate , growth, tiger grouper (Epinephelus fuscoguttatus)

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