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Pengaruh Perbedaan Waktu Awal Pemberian Pakan Buatan terhadap Pertumbuhan dan Aktivitas Enzim Pencernaan Larva Ikan Kerapu Bebek Cromileptes altivelis (Valenciennes 1828) Melianawati, Regina; Pratiwi, Rarastoeti; Astuti, Ni Wayan Widya
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3055

The effect of different initial time of artificial feeding to the growth and the activities of digestive enzymes of humpback grouper larvae Cromileptes altivelis (Valenciennes 1828).Humpback grouper is an economically marine finfish commodity, so it is important to scale up its culture. In aquaculture, feed is dominantly affecting to fish growth. Besides live feeds, theartificial feed is also given to fulfill the need of nutrient for larvae. However, the capability of larvae to digest the artificial feed is still limited because of its simplicity and immaturity of digestive system structure. Digestive enzymes are a biological indicator to figure out the capability of larvae to digest the artificial feed. This research was conducted to find out the influence of different initial time of artificial feeding to the growth and digestive enzyme activity of humpback grouper larvae. Larvae rearing were done in hatchery. Larvae were fed with live feed rotifers started in the evening of the second day. The treatment given in this research was the difference initial time of artificial feeding, i.e: (A) given at 8 days old larvae and (B) given at13 days old larvae. The variable observations were the growth and digestive enzyme activity of larvae. The results indicate that the different initial time of artificial feeding influenced to thegrowth and digestive enzyme activity of humpback grouper larvae. Total length, body weight, length of dorsal spine and ventral spine of 30 days old humpback grouper larvae on treatment A were 17.47Â±2.35 mm, 0.11Â±0.04 g, 6.83Â±0.45 mm and 5.07Â±0.50 mm, respectively, while on treatment B were 13.23Â±2.53 mm, 0.04Â±0.02 g, 5.73Â±1.11 mm and 4.48Â±0.50 mm, respectively. Up to 30 days old larvae, protease and amylase enzymes activities of larvae on treatment A was higher than on treatment B, while lipase enzymes activity of larvae on treatment B was higher than on treatment A. Therefore, the different initial time of artificial feeding gives the different effect on the growth and the activities of protease, amylase and lipase enzymes of humpback grouper larvae.Key words: artificial feed, growth, digestive enzyme activity, humpback grouper, larvae

PENGARUH PERBEDAAN WAKTU AWAL PEMBERIAN PAKAN BUATAN TERHADAP PERTUMBUHAN DAN AKTIVITAS ENZIM PENCERNAAN LARVA IKAN KERAPU BEBEK CROMILEPTES ALTIVELIS (VALENCIENNES 1828) Melianawati, Regina; Pratiwi, Rarastoeti; Astuti, Ni Wayan Widya
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3055

The effect of different initial time of artificial feeding to the growth and the activities of digestive enzymes of humpback grouper larvae Cromileptes altivelis (Valenciennes 1828).Humpback grouper is an economically marine finfish commodity, so it is important to scale up its culture. In aquaculture, feed is dominantly affecting to fish growth. Besides live feeds, theartificial feed is also given to fulfill the need of nutrient for larvae. However, the capability of larvae to digest the artificial feed is still limited because of its simplicity and immaturity of digestive system structure. Digestive enzymes are a biological indicator to figure out the capability of larvae to digest the artificial feed. This research was conducted to find out the influence of different initial time of artificial feeding to the growth and digestive enzyme activity of humpback grouper larvae. Larvae rearing were done in hatchery. Larvae were fed with live feed rotifers started in the evening of the second day. The treatment given in this research was the difference initial time of artificial feeding, i.e: (A) given at 8 days old larvae and (B) given at13 days old larvae. The variable observations were the growth and digestive enzyme activity of larvae. The results indicate that the different initial time of artificial feeding influenced to thegrowth and digestive enzyme activity of humpback grouper larvae. Total length, body weight, length of dorsal spine and ventral spine of 30 days old humpback grouper larvae on treatment A were 17.47±2.35 mm, 0.11±0.04 g, 6.83±0.45 mm and 5.07±0.50 mm, respectively, while on treatment B were 13.23±2.53 mm, 0.04±0.02 g, 5.73±1.11 mm and 4.48±0.50 mm, respectively. Up to 30 days old larvae, protease and amylase enzymes activities of larvae on treatment A was higher than on treatment B, while lipase enzymes activity of larvae on treatment B was higher than on treatment A. Therefore, the different initial time of artificial feeding gives the different effect on the growth and the activities of protease, amylase and lipase enzymes of humpback grouper larvae.Key words: artificial feed, growth, digestive enzyme activity, humpback grouper, larvae

PENGARUH LAMA WAKTU YANG BERBEDA PADA TRANSPORTASI BENIH IKAN KERAPU SUNU Plectropomus leopardus DENGAN SISTEM TERTUTUP Suko Ismi; Yasmina Nirmala Asih; Afifah Nasukha; Ni Wayan Widya Astuti
JFMR (Journal of Fisheries and Marine Research) Vol 4, No 3 (2020): JFMR VOL 4 NO 3
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.03.4

Ikan kerapu sunu Plectropomus leopardus merupakan komoditas perikanan laut yang bernilai ekonomis tinggi dengan harga yang mahal. Benih telah dapat diproduksi mulai dipasarkan ukuran 3,0 cm, proses transportasi sangat diperlukan mengingat konsumen yang memerlukan benih kerapu sunu untuk usaha pendederan atau budidaya yang tempatnya jauh dari sumber benih. Penelitian ini bertujuan mengetahui sampai berapa lama waktu tempuh yang dapat menghasilkan benih dengan kelangsungan hidup tinggi dan kondisi ikan sehat. Transportasi benih ikan kerapu sunu dilakukan dengan sistem tertutup, dengan menggunakan mobil bergerak, setelah 6 jam perjalanan oksigen dalam kantong plastik diganti, kemudian dikemas kembali seperti semula untuk melanjutkan perjalanan sesuai perlakuan. Benih ikan kerapu sunu yang digunakan ukuran 3,15±0,54 cm, dengan kepadatan 250 ekor per bok. Penelitian menggunakan rancangan acak lengkap dengan lima perlakuan dan tiga kali ulangan, dengan perlakuan beda waktu transportasi: A.12 jam; B. 16 jam; C.20 jam; D. 24 jam dan E. 30 jam. Hasil menunjukan lama waktu transportasi pada perlakuan 12 jam hingga 24 jam tidak berbeda nyata menghasilkan kelangsungan hidup 98,0 – 100% tetapi berbeda nyata (P<0,05) dengan tranportasi selama 30 jam dengan kelangsungan hidup 79,9%.

THE GROWTH PATTERN OF BLACKSADDLED CORALGROUPER LARVAE (Plectropoma laevis LACEPÈDE, 1801) AND THEIR CONSUMPTION RATE TO ZOOPLANKTON ROTIFER (Brachionus rotundiformis) Regina Melianawati; Ni Wayan Widya Astuti; Bejo Slamet
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 4 No. 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Blacksaddled coralgrouper Plectropoma laevis has been started to conserve to prevent from over exploitation. The study purposes were to determine the growth pattern during larvae to juvenile stage of Blacksaddled coralgrouper and their consumption rate to zooplankton rotifers Brachionus rotundiformis. Domesticated broodstocks have been rearing in 100,000 l concrete tanks. The eggs from spawning broodstocks were hatched and the larvae have been kept to juvenile. Larvae rearing was done in 6,000 l concrete tanks. During the rearing period, larvae were fed with zooplankton rotifer, artemia and artificial food. Parameters measured were eggs and oil globule diameter, larval total length, length of larval dorsal fin and ventral fin, the number of zooplankton that consumed by larvae and water temperature. Microscopic method was used to measure the parameters. The result showed that eggs and oil globule diameter ranged in 800-850 μm and 168-200 μm, respectively. Total length of newly hatched larvae was 2.53±0.13 mm. The growth pattern of total length from larva to juvenile stage was exponential, while the growth pattern of dorsal fin and ventral fin length was linear. Larvae grew very fast after 35 days old. The pattern of larval consumption rate to zooplankton rotifers was linear. Time period from larvae to juvenile stage was 45-55 days on water temperature 27-29oC.Keywords: growth, consumption rate, rotifers, larvae, Blacksaddled coralgrouper

EFFECT ON DIETARY PROTEIN AND FEEDING RATE ON GROWTH OF TIGER GROUPER (Epinephelus fuscoguttatus) JUVENILE Muhammad Marzuqi; Ni Wayan Widya Astuti; Ketut Suwirya
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 4 No. 1 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The grouper fish culture was developed after its succesful seed production in hatchery well known. In grow-out culture grouper, the protein requirement and feeding rate have to know well in order to understand the effectiveness on feed utilization . The experiment was designed by factorial design with the first factor as 3 dietary protein (36%, 42%, 48%) and the second factor as 3 feeding rate ( 1,5%, 2,0%, 2,5%). Ten of juvenile tiger grouper (102, 51-102, 73 g of body weight) were stocked in 27 polycarbonate tanks with 400 l volume each. Each tank was equiped with aeration and flow-through water system. Fish was fed three time per day i.e. 08:00, 11:30 and 15:30 Central Indonesia Standard Time for 16 weeks. Every week was measured body weight, survival rate, and feed quantity. The parameter used in this experiment were weight gain, spesific growth rate, feed efficiency and survival rate. The results of the experiment showed that the combination of protein level (36%, 42%, 48%) with feed ratio (1.5%, 2.0%, 2.5%) gave response on growth, weight gain and survival rate. Combination dietary protein level of 48% and feed ratio 1.5% gave higher weight gain and survival rate with values of 104.05 ± 5.89% and 96.67 ± 5.57 %, respectively.Keywords: Dietary protein and feeding rate , growth, tiger grouper (Epinephelus fuscoguttatus)

PERBEDAAN DOSIS PROBIOTIK Bacillus cereus DALAM PAKAN TERHADAP PERTUMBUHAN DAN AKTIVITAS ENZIMATIS JUVENIL IKAN KERAPU SUNU (Plectropomus leopardus) Marzuqi, Muhammad; Astuti, Ni Wayan Widya; Muzaki, Ahmad; Giri, Nyoman Adiasmara
Media Akuakultur Vol 17, No 1 (2022): (Juni, 2022)
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.17.1.2022.33-41

Kerapu sunu (Plectropomus leopardus) merupakan ikan laut ekonomis penting dan mempunyai prospek yang baik untuk budidaya. Sistem pencernaan ikan ini sangat dipengaruhi oleh pemanfaatan nutrien pakan yang digunakan. Pemanfaatan probiotik dapat mengaktifkan aktivitas enzim dalam pencernaan ikan. Tujuan penelitian untuk mendapatkan dosis probiotik Bacillus cereus yang tepat dalam pakan terhadap pertumbuhan dan aktivitas enzimatis juvenil ikan kerapu sunu. Penelitian dilakukan dengan 4 perlakuan dosis probiotik dalam pakan, yaitu 0%, 0,5%, 1,0% dan 1,5% dengan 4 kali ulangan. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Pakan uji diformulasikan dengan kandungan protein 48,32-49,25% dan kandungan lemak 11,97-12,98%. Juvenil ikan kerapu sunu memiliki berat rata rata 5,7±0,3 g dan panjang awal rata-rata 7,3±0,3 cm, juvenil dipelihara dalam 16 bak polikarbonat bervolume 30 L dengan sistem air mengalir dan kepadatan 12 ekor/bak. Lama pemeliharaan selama 8 minggu. Ikan diberi pakan percobaan dengan frekuensi 2 kali sehari. Parameter yang diamati pada penelitian ini adalah pertumbuhan, laju pertumbuhan spesifik, kelangsungan hidup, efisiensi pakan, viabilitas bakteri dalam pakan dan aktivitas enzimatis pada saluran pencernaan. Hasil penelitian menunjukkan bahwa pemberian probiotik dalam pakan ikan kerapu sunu mampu meningkatkan pertumbuhan, efisiensi pakan, viabilitas bakteri probiotik, aktivitas enzim protease dan aktivitas enzim lipase pada usus. Dosis pemberian probiotik 1,5% dalam pakan menunjukkan hasil lebih baik dibanding pakan lainnya (P<0,05).

Pengaruh Pemberian Pakan Kombinasi Nannochloropsis oculata Dan Terasi Dengan Dosis Yang Berbeda Terhadap Laju Pertumbuhan Rotifera (Branchionus Plicatilis) Paramitha, Komang Putri Regina; Amelia, Jasmine Masyitha; Fain, Hamdanul; Astuti, Ni Wayan Widya
Journal of Tropical Marine Science Vol 7 No 2 (2024): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v7i2.4150

Penelitian yang dilakukan bertujuan untuk mengetahui pengaruh kombinasi pakan Chlorella dan terasi yang berbeda terhadap laju pertumbuhan populasi rotifera (Branchiounus plicatilis). Penelitian dilakukan dari bulan Maret sampai dengan bulan Mei 2023 di Kawasan Konservasi Ilmiah Biota Laut Badan Riset dan Inovasi Nasional Gondol Bali. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan A sebagai kontrol hanya diberikan pakan Chlorella sebanyak 6,66 x 105 sel/ml perlakuan B yakni Chlorella 6,66 x 105 sel/ml dan terasi 1gr/L. perlakuan C yakni Chlorella 6,66 x 105 sel/ml dan terasi 2gr/L, dan perlakuan D yakni Chlorella 6,66 x 105 sel/ml dan terasi 3gr/L. dengan dosis pemberian pakan kombinasi dilakukan setiap 2 hari sekali. Parameter yang dianalisis hanya laju pertumbuhan populasi dan mencari dosis yang terbaik. Data di olah menggunakan One Way ANOVA dengan tingkat kepercayaan 95% dan jika hasil menunjukkan berbeda signifikan maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pertumbuhan populasi Rotifera meningkat dengan pemberian kombinasi pakan Chlorella ditambah terasi dengan dosis 1gr/L. pada perlakuan B pertumbuhan populasi memiliki puncak kepadatan sebanyak 53 ind/ml dengan laju pertumbuhan 0,8741 ind/ml/hari. Jadi dosis terasi terbaik yang diberikan untuk kultur rotifera sebanyak 1gr/L.

The use of technical chlorine for seawater sterilization in the rearing of barramundi larvae Lates calcarifer Astuti, Ni Wayan Widya; Mastuti, Indah; Sedana, I Made; Sunarto, Sunarto; Mustakim, Mustakim; Suwitra, I Nyoman; Mujiono, Mujiono; Kurdi, Kurdi; Dewi, Luh Yuliani; Tantra, Kadek Mas; Katimin, Katimin; Shadiq, Ja'far; Muzaki, Ahmad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan 2025: Special Issue ICFM
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46920

Disease prevention is very important in the rearing of barramundi larvae, one of which is by sterilizing the seawater using technical chlorine. This study aimed to determine the effect of seawater sterlization use technical chlorine for rearing barramundi larvae on growth, survival, and water quality. The treatment in this study was different seawater used for water exchange during larval rearing. Seawater was sterilized with 25 ppm technical chlorine for 24 hours. Before use, the water was neutralized using 12.5 ppm sodium thiosulfate. As a control in this study, direct seawater or untreated sea water was used for water exchange. The study was conducted with 3 replications. The parameters observed in this study were growth, survival, virus monitoring, growth variation, and water quality. Sterilization of seawater with 25 ppm technical chlorine used for rearing larvae of barramundi has no significant effect on growth, survival, growth variation and water quality of rearing larvae. The use of filtered seawater is recommended in the sterilization of seawater for rearing larvae of barramundi. Increasing the dose of chlorine or using chlorine which has a high active substance could increase effectiveness of water sterilization.Keywords:Technical chlorineSterilizationRearing larvaeBarramundiLates calcarifer

The use of technical chlorine for seawater sterilization in the rearing of barramundi larvae Lates calcarifer Astuti, Ni Wayan Widya; Mastuti, Indah; Sedana, I Made; Sunarto, Sunarto; Mustakim, Mustakim; Suwitra, I Nyoman; Mujiono, Mujiono; Kurdi, Kurdi; Dewi, Luh Yuliani; Tantra, Kadek Mas; Katimin, Katimin; Shadiq, Ja'far; Muzaki, Ahmad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan 2025: Special Issue ICFM
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46920

Disease prevention is very important in the rearing of barramundi larvae, one of which is by sterilizing the seawater using technical chlorine. This study aimed to determine the effect of seawater sterlization use technical chlorine for rearing barramundi larvae on growth, survival, and water quality. The treatment in this study was different seawater used for water exchange during larval rearing. Seawater was sterilized with 25 ppm technical chlorine for 24 hours. Before use, the water was neutralized using 12.5 ppm sodium thiosulfate. As a control in this study, direct seawater or untreated sea water was used for water exchange. The study was conducted with 3 replications. The parameters observed in this study were growth, survival, virus monitoring, growth variation, and water quality. Sterilization of seawater with 25 ppm technical chlorine used for rearing larvae of barramundi has no significant effect on growth, survival, growth variation and water quality of rearing larvae. The use of filtered seawater is recommended in the sterilization of seawater for rearing larvae of barramundi. Increasing the dose of chlorine or using chlorine which has a high active substance could increase effectiveness of water sterilization.Keywords:Technical chlorineSterilizationRearing larvaeBarramundiLates calcarifer

Potensi Antiparasitik Herbal Ekstrak Herbal Terhadap Lintah Laut (Zeylanicobdella arugamensis) Pada Juvenil Ikan Kerapu Hibrida Cantang (Epinephelus fuscoguttatus X E. lanceolatus) Mastuti, Indah; Sadewa, Ketut M. Arya; Ansari, Mohamad; Haryanto, Slamet; Zailani, Ahmad; Suwitra, I Nyoman; Ariani, Ni Kadek; Kurdi, Kurdi; Miniartini, Made; Mustaqim, Mustaqim; Sedana, I Made; Sunarto, Mujiono; Astuti, Ni Wayan Widya; Mahardika, Ketut
Media Akuakultur Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.1.2025.1-13

Lintah laut adalahektoparasit yang sangat merugikan bagbudidaya ikan kerapu di Bali Utara. Penelitian ini bertujuan menggali potensi antiparasitik jenis 76 tanaman dalam mengendalikan infeksi lintah laut (Zeylanicobdella arugamensis) pada juvenil ikan kerapu hibrida cantang (Epinephelus fuscoguttatus x E. lanceolatus). Sebanyak 76 jenis tanaman diekstrak menggunakan pelarut air laut steril untuk uji in vitro tahap 1 sebagai skrining awal, dilanjutkan dengan uji in vitro tahap 2 dan uji in vivo tahap 1 dan tahap 2. Uji in vivo dilakukan dengan merendam lintah laut dalam ektrak herbal 1000 ppm selama dua jam. Hasil uji in vitro tahap 1 mendapakan 11 tanaman berpotensi antiparasitik, yaitu lada putih, lada hitam, bunga cengkeh, lengkuas, cabai Jawa, jahe hitam, kulit buah manggis, daun sawo Manila, daun delima, daun brotowali dan batang brotowali. Berdasarkan uji tersebut dilakukan uji in vitro tahap 2 untuk mengetahui konsentrasi minimal yang melemahkan lintah laut. Uji in vitro tahap 2 menunjukkan bahwa konsentrasi minimum (500 ppm) hanya diperoleh dari tanaman lada putih. Uji in vivo dilakukan dengan menggunakan ikan kerapu cantang yang yang terinfeksi lintah laut. Uji in vivo tahap 1 menunjukkan bahwa sembilan (lada putih, lada hitam, bunga cengkeh, lengkuas, cabai Jawa, kulit buah manggis, daun sawo Manila, daun delima, daun brotowali) dari sebelas herbal tersebut belum mampu melepaskan lintah laut namun mematikan bagi ikan. Sedangkan uji in vivo tahap 2 menunjukkan sekaligus menyimpulkan bahwa ekstrak herbal, yaitu jahe hitam (Curcuma aeruginosa Roxb.) dan daun brotowali efektif (Tinospora cordifolia) dalam air laut konsentrasi 1000 ppm.Sea leeches are the major ectoparasites that infect grouper fish. This study explores the antiparasitic potential of 76 plants in controlling sea leech. The plants were extracted using sterile seawater and used in the in vitro test 1 (initial screening), followed by in vitro test 2 and in vivo test 1 and 2. The in vivo test was carried out by soaking sea leeches in 1000 ppm herbal extractsfor two hours. The results of the in vitro test 1 found that 11 plants had antiparasitic potential (white pepper, black pepper, clove flowers, galangal, Javanese chili, black ginger, mangosteen peel, Manila sapodilla leaves, pomegranate leaves, brotowali leaves and brotowali stems). Based on initial test, the in vitro test 2 was conducted to determine the minimum concentration. The in vitro test 2 showed that the minimum concentration (500 ppm) was obtained from white pepper . The in vivo test was conducted using infected fish. The in vivo test 2 showed that nine (white pepper, black pepper, clove flowers, galangal, Javanese chili, mangosteen peel, Manila sapodilla leaves, pomegranate leaves, brotowali leaves) herbals were not able to release leeches but deadly to the fish. Meanwhile, the in vivo test 2 concluded that black ginger (Curcuma aeruginosa Roxb.) and brotowali leaves (Tinospora cordifolia) in sea water at a concentration of 1000 ppm were effective in releasing leeches within 10 minutes and non toxic to fish.

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