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Tridjoko Tridjoko
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IMPLANTATION OF LHRH-a HORMONE TO STIMULATION OF GONAD DEVELOPMENT OF HUMPBACK GROUPER SECOND GENERATION ( F-2 ) Tridjoko Tridjoko
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.623 KB) | DOI: 10.29244/jitkt.v5i2.7561

Abstract

This study aimed to determine the effect of LHRH-a hormone implantation on gonadal development of humpback grouper, Cromileptes altivelis 2nd generation (F-2). Individuals used were 20 female grouper fish with weight range of 600-800 gram/fish and total length range of 28.0-30.0 cm. The treatment used 5 fish each with tagging. Grouper fishes were stocked into the 75 m3 circular concrete tank. Hormone of LHRH-a with dosage of 50 μg/kg body weight was used as implant treatment of (A) 1 time,, (B) 2 times, (C) 3 times, and (D) without implant. The results showed that implantation of LHRH-a hormone were significantly stimulate gonad development of humpback grouper second generation (F-2). Treatment of 3 times implant every month showed the best results produced  oocyte diameter up to 480 μm, while the control of oocyte diameter only reached <400 μm. Keywords: LHRH-a hormone, implantation, Humpback grouper, gonad development
GENETIC VARIATION OF HUMPBACK GROUPER (Cromileptes altivelis) ON F1 AND F3 GENERATIONS Sari Budi Moria Sembiring; Tridjoko Tridjoko; Haryanti Haryanti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.469 KB) | DOI: 10.29244/jitkt.v5i1.7755

Abstract

Hatchery and culture technology of Humpback grouper has been developed. However,  sometimes it is still found constraint in sedd production and caused instability of production due to failure by many factors. Breeding program become necessary to provide seed with high quality and good genetic variation. Quantitative characteristic selection is one of breeding program to improve productivity in grouper aquaculture. The aim of this research was to produce good phenotyphic and genotypic quality of Humpback grouper candidate broodstock. Selection of Humpback grouper F1 and F3 was done based on quantitative characteristic (body length and weight) and genotyphic characteristic by using ramdom amplified polymorphism DNA (RAPD) method.  The results of conventional selection were found candidate broodstock of Humpback grouper F1 and F3 with body weight range from 170-210 g and 160-170 g consecutively.  Heterozygosity values of Humpback grouper analized by RAPD of F1 and F3 were 0.7940 and 0.7749 consecutively and it was not significantly different. This value emphasis that population of this F3 Humpback grouper was still good to grow for broodstock. Keywords: genetic variation, humpback grouper, F1, F3.
REPRODUCTION PERFORMANCE OF HUMPBACK GROUPER (Cromileptes altivelis) FROM WILD BROODSTOCK (F-0), FIRST GENERATION BROODSTOCK (F-1), AND SECOND GENERATION BROODSTOCK (F-2) Tridjoko Tridjoko
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 2 No. 2 (2010): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.601 KB) | DOI: 10.29244/jitkt.v2i2.7847

Abstract

Grouper seeding technology still relies on the broodstock from the ocean, eventhough, the existence of the broodstock is rare and difficult to obtain. The broodstock takes a long time to mature and spawn. Therefore, to anticipate the scarcity humpback grouper from wild, it is important to produce broodfish from aquaculture. This study was aimed to know the reproduction performance of humpback grouper wild broodstock F-0, first generation broodstock (F-1) and second generation broodstock (F-2). The cultured of humback grouper broodstock used three tanks 75 m3 in volume (tank I, II and tank III). Tank I: reared 35 fishes broodstock F-0. Tank II and tank III for reared each 50 fishes F-1 and F-2 broodstock. Food for broodstock from the wild (F-0) was given from fresh trash fish, squid, and added vitamin mixture, vitamin C and vitamin E. Meanwhhile, food given to F-1 and F-2 broodstock was a commercial dry pellet (PG 9-10) that contains following nutrients: protein content of 43%, min. fat of 9%, ash content of 13%, fiber levels of 2%, and moisture content of 12%. Mixture of vitamin C and E were also added. The results showed that the humpback grouper F-0, F-1 and F-2 were cultured in 75 m3 concrete tank was successfully spawn with the fertilization rate of 87%, 78% and 45% respectively. This result proves that the broodstock from F-0 gaved the best results.Keywords: Humpback grouper reproduction, wild broodstock (F-0), first generation (F-1), seccond generation (F-2)
Co-Authors Haryanti Haryanti Sari Budi Moria Sembiring