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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis BIOTROPIA - The Southeast Asian Journal of Tropical Biology Marine Research in Indonesia Jurnal Riset Akuakultur
Sari Budi Moria Sembiring
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Library & Information Science Veterinary

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REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra) Sembiring, Sari Budi Moria; Wibawa, Gigih Setia; Giri, I Nyoman Adiasmara; Hutapea, Jhon Harianto; Haryanti, -
Marine Research in Indonesia Vol 43, No 1 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.996 KB) | DOI: 10.14203/mri.v43i1.267

Abstract

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.
REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra) Sembiring, Sari Budi Moria; Wibawa, Gigih Setia; Giri, I Nyoman Adiasmara; Hutapea, Jhon Harianto; Haryanti, -
Marine Research in Indonesia Vol 43 No 1 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.996 KB) | DOI: 10.14203/mri.v43i1.267

Abstract

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.
GENETIC VARIATION OF HUMPBACK GROUPER (Cromileptes altivelis) ON F1 AND F3 GENERATIONS Sari Budi Moria Sembiring; Tridjoko Tridjoko; Haryanti Haryanti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.469 KB) | DOI: 10.29244/jitkt.v5i1.7755

Abstract

Hatchery and culture technology of Humpback grouper has been developed. However,  sometimes it is still found constraint in sedd production and caused instability of production due to failure by many factors. Breeding program become necessary to provide seed with high quality and good genetic variation. Quantitative characteristic selection is one of breeding program to improve productivity in grouper aquaculture. The aim of this research was to produce good phenotyphic and genotypic quality of Humpback grouper candidate broodstock. Selection of Humpback grouper F1 and F3 was done based on quantitative characteristic (body length and weight) and genotyphic characteristic by using ramdom amplified polymorphism DNA (RAPD) method.  The results of conventional selection were found candidate broodstock of Humpback grouper F1 and F3 with body weight range from 170-210 g and 160-170 g consecutively.  Heterozygosity values of Humpback grouper analized by RAPD of F1 and F3 were 0.7940 and 0.7749 consecutively and it was not significantly different. This value emphasis that population of this F3 Humpback grouper was still good to grow for broodstock. Keywords: genetic variation, humpback grouper, F1, F3.
PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI Ahmad Muzaki; Sari Budi Moria Sembiring; Ida Komang Wardana; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.364 KB) | DOI: 10.15578/jra.12.2.2017.131-137

Abstract

Pertumbuhan dan sintasan larva dan benih kerapu sunu turunan ketiga (F-3) dari induk turunan kedua (F-2) hasil seleksi telah diamati di Balai Penelitian dan Pengembangan Budidaya Laut, Gondol. Induk F-2 diseleksi menggunakan marka indikator tumbuh cepat PL-03 (alel tunggal berukuran 370 bp). Penelitian ini bertujuan untuk mengevaluasi keragaan pertumbuhan dan sintasan larva dan benih kerapu sunu F-3 serta pewarisan sifat tumbuh cepat dari induk kerapu sunu (F-2) kepada benih kerapu sunu F-3. Pembenihan menggunakan induk kerapu sunu F-2 dengan sifat tumbuh cepat (membawa marka PL-03) dan tanpa sifat tumbuh cepat. Telur dan larva yang dihasilkan dipelihara sebagai turunan F-3 hingga berukuran benih dan dibesarkan selama 10 bulan pemeliharaan. Hasil pengamatan sintasan larva kerapu sunu F-3 dari induk membawa marka PL-03 adalah sebesar 10,6% ± 9,1%; sedangkan larva F-3 dari induk yang tidak membawa sifat tumbuh cepat hanya sebesar 2,7% ± 1,7%. Benih F-3 dari induk membawa marka PL-03 dapat dipanen saat mencapai ukuran 2,5-3,0 cm pada umur 38 ± 2 hari, sedangkan benih F-3 dari induk tanpa marka PL-03 pada umur 40 ± 1 hari. Selanjutnya benih kerapu sunu F-3 dari induk yang mempunyai marka PL-03 yang dipelihara selama 10 bulan memiliki laju pertumbuhan harian lebih baik (3,11%) dibandingkan benih kerapu sunu F-3 tanpa marka PL-03 (3,01%). Sintasan benih kerapu sunu F-3 dengan marka PL-03 tidak berbeda nyata dengan yang tidak mempunyai marka PL-03. Persentase benih F-3 turunan dari induk F-2 dengan sifat tumbuh cepat yang membawa marka PL-03 sebesar 45%.The growth and survival rate of the third generation (F-3) of coral trout grouper larvae and fry from the genetically selected broodstock were observed at the Institute for Mariculture Research and Development, Gondol. The F-2 broodstock were selected using the fast-growing marker indicator, PL-03 (single allele of 370 bp in size). The aim of this study was to determine the growth performance and survival rate of larvae and fry of F-3 coral trout groupers, as well as inheritance of fast growth trait from the F-2 broodstock to the F-3. The eggs and larvae of F-3 produced from F-2 broodstock with fast growth trait (having PL-03 marker) and without fast growth trait were reared to reach fry stage for 10 months. The results showed that the survival rate of the F-3 larvae from broodstock with PL-03 marker was 10.6% ± 9.1%, while F-3 larvae from the broodstock without fast growth trait only reached 2.7% ± 1.7%. Fry (F-3) with PL-03 marker reached the optimum harvest size of 2.5-3.0 cm at 38 ± 2 days old, while the F-3 fry of broodstock without PL-03 marker took relatively longer to reach that size (40 ± 1 days age). The F-3 from the broodstock with PL-03 marker reared for 10 months had better growth rate (3.11%) compared to F-3 fry without PL-03 marker (3.01%). The survival rate of F-3 fry with PL-03 marker was not significantly different to F-3 fry without PL-03 marker. The percentage of F-3 fry from the broodstock of F-2 with fast growth trait was 45%.
The Survival, Growth, and Accelerating Morphological Development of Stichopus horrens are Affected by the Initial Larval Stocking Densities Sembiring, Sari Budi Moria; Setiawati, Ketut Maha; Widiastuti, Zeny; Hutapea, Jhon Harianto; Gunawan, Gunawan; Setiadi, Ananto; Haryanti; Giri, Nyoman Adi Asmara
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.233-240

Abstract

Stichopus horrens is highly exploited due to their use as a pharmaceutical ingredient. Since then, this species has become extinct; therefore, it is necessary to start aquaculture. Gonad maturation and broodstock spawning succeeded, but the optimum larval-rearing stocking density has not yet been determined. Therefore, the present study aimed to determine the optimal stocking density to improve survival and accelerate the development of larval morphology. Three treatments were used: 100, 150, and 200 larvae L-1, each with four replicates. Twelve 100-litre plastic containers filled with 80 litres of seawater as larval rearing media were placed in a concrete tank with a water-bath system using a heater and a thermostat (29.0°C±1.0°C). The larvae were fed a mixture of microalgae (Chaetoceros muelleri, Isochrysis galbana, and Nitzchia sp.) twice a day-1. The data collected included survival and growth rates, larval morphological development, and water quality. The fastest metamorphose from auricularia to the doliolaria stage occurred in 100 larvae L-1, 15 days after hatching. The highest survival rate, growth rate, and percentage of larvae metamorphose to the doliolaria stage were obtained in the 100 larvae L-1 as 26.38%, 26.4 µm day-1, and 65.27%, respectively, and were significantly different (P<0.05). A stocking density of 100 larvae L-1 was optimal for promoting survival and growth and accelerating the morphological development of auricularia to the doliolaria stage.
POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Khotimah, Fitriyah Husnul; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri; Nuryati, Sri; Arfah, Harton; Sugama, Ketut; Permana, Gusti Ngurah; Sembiring, Sari Budi Moria; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Abstract

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.
Effect of Salinity on the Survival, Growth and Immunity Rate of Juvenile Sea Cucumbers (Holothuria scabra) Sembiring, Sari Budi Moria; Wibawa, Gigih Setia; Hutapea, Jhon Harianto; Giri, I Nyoman Adiasmara
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.779 KB) | DOI: 10.11598/btb.2019.26.3.1041

Abstract

Sea cucumber (Holothuria scabra) is an important species as food and medicine, especially for the Chinese market. It is a stenohaline and osmoconforming organism with a low level of tolerance to salinity change. Salinity of the medium is one of the environmental factors that affect the physiology and survival of juvenile sea cucumbers. This study was aimed at assessing the effect of different salinities on the growth, survival and immunity rate of juvenile sea cucumbers (Holothuria scabra) and also determining the suitable salinity level for the optimal growth, survival and immunity rate of the sea cucumbers. This experiment used a completely randomized design with 5 salinity treatments: 24, 29, 34, 39, and 44 ppt with 3 replications using fifteen 30 L fiberglass tanks. The juvenile sea cucumbers measured 4.4 ± 0.2 cm in total length and 5.6 ± 0.3 g in body weight. The juveniles were raised at 15 individuals/tank, fed with cultured fresh benthos once a day in the afternoon. Coelomate was taken from the sea cucumber juveniles from each tank and used to determine the immunity rate and also for the osmolality. The data were analyzed using ANOVA followed by Tukey's test at 5% level of significance. The salinity of the medium significantly affected (p < 0.05) the growth, survival rate and immunity rate of sea cucumber juveniles. The 24–34 ppt salinity can support survival rate up to 100%, high growth (6.47–7.10 g) and immunity rate (27–76 × 10⁴ phagocytic cells/mL), while the 44 ppt salinity has resulted in not only a low survival rate (55.60%), but also had a bad effect on osmolality (303 ± 3.5 mOsm/kg), growth (3.12 ± 0.34 g), and immunity (209 × 10⁴ phagocytic cells/mL).
Co-Authors - Haryanti Ahmad Muzaki Dinar Tri Soelistyowati Gigih Setia Wibawa Giri, I Nyoman Adiasmara Giri, I Nyoman Adiasmara Giri, I Nyoman Adiasmara Giri, Nyoman Adi Asmara Gunawan Gunawan Gusti Ngurah Permana Harton Arfah Haryanti Haryanti Haryanti Haryanti Haryanti Ida Komang Wardana Jhon Harianto Hutapea Ketut Maha Setiawati Ketut Sugama Khotimah, Fitriyah Husnul Setiadi, Ananto Sri Nuryati Tridjoko Tridjoko Widiastuti, Zeny