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All Journal BALI MEDICAL JOURNAL (BMJ) The Indonesian Biomedical Journal
Andi Asadul Islam
Faculty of Medicine, Hasanudin University, Makasar-Indonesia
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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MAXIMIZATION OF DNA DAMAGE TO MGMT(+) EGFR(+) GBM CELLS USING OPTIMAL COMBINATION OF TEMOZOLOMIDE-ANTI EGFR MONOCLONAL ANTIBODY NIMOTUZUMAB Inggas, Made Agus Mahendra; Wahjoepramono, Eka J.; Maliawan, Sri; Islam, Andi Asadul
BALI MEDICAL JOURNAL Vol 4 No 3 (2015)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.158 KB)

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Background: Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor in adultswith dismal prognosis due to the unavailability of an effective therapy. Up to now, there had been no definitive studies published on EGFR inhibition therapy as a chemosensitizer for GBM therapy using Temozolomide (TMZ). This study aims to reveal the most effective method and timing to administer TMZ anti EGFR targeted therapy which causes maximal DNA damage on GBM cells. Methods: Various regimens of anti EGFR monoclonal antibody Nimotuzumab (NMZ) was administered in different combinations with TMZ, performed on U87MG MGMT(+) EGFR(+) cells. The effectiveness of the combinations were evaluated by measuring yH2AX levels which reflects the degree of DNA damage. One-way Anova and LSD tests were performed to determine the effects of each treatment with p
THE ROLE OF RECOMBINANT IL-10 ON THE SERUM LEVEL OF TNF-?, ONE HOUR POST TRAUMATIC BRAIN INJURY OF THE WISTAR RAT Adhimarta, Willy; Islam, Andi Asadul; Maliawan, Sri; Lowrence, Gatot S.; Patellongi, Ilhamjaya
BALI MEDICAL JOURNAL Vol 4 No 1 (2015)
Publisher : BALI MEDICAL JOURNAL

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Background: Brain injury often occurs not only primary brain injury, but often also occur secondary brain injury. Inflammation is a process that occurs immediately after trauma characterized by activation of the mediator substance. TNF-? is a major cytokinee involved in the inflammatory processes that have adverse effects if the serum level are excessive. There needs to be a balance of the inflammatory process in the brain injury so things that harm does not occur. As anti-inflammatory IL- 10 plays an important role in maintaining the balance. The objective of this study is to determine the effect of IL-10 intervention as an anti -inflammatory will decrease the serum level of TNF-? in traumatic brain injury. Material And Method: Experimental Study in the Rattus Wistar rats, post test control group design, male, aged 3-4 months, with body weight (BW) 300-400g, were obtained from the Laboratory Animal Faculty of Medicine, University of Hasanuddin as much as 24 tails, which is the result of breeding. Subjects were divided into four groups, each group of six rats, treated with controlled cortical impact model (Feeney’s weight-drop) of traumatic brain injury. Blood taken with capillary tube in retro-orbita plexus or sinus.This study has approved by ethical clearance for research. Results: Levels of TNF-? group of rats 1 hour post-trauma without administration of recombinant IL-10 (28.58 ± 7.28) pg / mL; was significantly higher (p
MLC 901 Decreases HSP-70, MMP-9, Cerebral Infarction Volume and Functional Outcome in Acute Ischemic Stroke Rat Model Hunaifi, Ilsa; Bintang, Andi Kurnia; Tammasse, Jumraini; Wahid, Isra'; Hatta, Mohammad; Islam, Andi Asadul; Zainuddin, Andi Alfian; Sugianto, Paulus
The Indonesian Biomedical Journal Vol 17, No 3 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i3.3610

Abstract

BACKGROUND: Acute ischemic stroke (AIS) is usually treated with thrombolysis, however the percentage of patients receiving this therapy is not quite low. Therefore, it is necessary to find alternative therapy using neuroprotective agent such as Moleac (MLC) 901. Heat shock proteins (HSP)-70 and matrix metalloproteinase (MMP)-9 are usually related to AIS due to the triggered stroke-induced physiological stress. However, the effect of MLC 901 on Hsp70 mRNA expression, HSP-70 and MMP-9 remains unclear. This study was conducted to determine the effect of MLC 901 on those three parameters in relation to cerebral infarction volume and functional outcomes in an AIS model.METHODS: Rats were induced with AIS using unilateral common carotid artery occlusion (UCAO) and received three different treatments: 43.2 mg/200 gBW MLC 901, 21.6 mg/200 gBW MLC 901, and sodium carboxymethyl cellulose (CMC-Na), that were administered orally for 14 days. HSP-70 and MMP-9 protein levels were assessed using enzyme-linked immunosorbent assay (ELISA), and Hsp70 mRNA expression was assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Foot fault scores for evaluation functional outcome and infarction volume were assessed by ImageJ.RESULTS: AIS-induction increased HSP-70, MMP-9, and Hsp70 mRNA expression within 24-48 h. MMP-9, HSP-70 , and Hsp70 mRNA expression were reduced by MLC 901. MLC 901 at dose of 43.2 mg/200 gBW and 21.6 mg/200 gBW were effective in reducing these levels compared to the control. MLC 901 improved functional outcomes and decreased cerebral infarction volume. Moreover, a dosage of 43.2 mg/200 gBW was more effective in reducing Hsp70 mRNA expression and HSP-70, improving functional outcomes, and reducing cerebral infarction volume than a dosage of 21.6 mg/200 gBW, but not MMP-9 protein.CONCLUSION: MLC 901 effectively decreased Hsp70 mRNA expression, HSP-70 and MMP-9 protein levels, infarct volume, and functional outcomes. MLC 901 could be a potential therapeutic agent for AIS.KEYWORDS: MLC 901, HSP-70, MMP-9, acute ischemic stroke
Co-Authors Andi Alfian Zainuddin Andi Kurnia Bintang, Andi Kurnia Eka J. Wahjoepramono Gatot S. Lowrence, Gatot S. Hatta, Mohammad Ilhamjaya Patellongi Ilsa Hunaifi, Ilsa Made Agus Mahendra Inggas, Made Agus Mahendra Paulus Sugianto, Paulus Sri Maliawan Tammasse, Jumraini Wahid, Isra' Willy Adhimarta