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All Journal Tropical Medicine Journal Sari Pediatri Paediatrica Indonesiana JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Jurnal Gizi Klinik Indonesia Majalah Farmaseutik Medica Hospitalia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Berita Kedokteran Masyarakat

Ida Safitri Laksanawati

Departemen Ilmu Kesehatan Anak, Fakultas Kedokteran, Kesehatan Masyarakat Dan Keperawatan Universitas Gadjah Mada/ RSUP Dr. Sardjito, Yogyakarta

Author-ID : 2892961

Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Medicine & Pharmacology Nursing Public Health

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The HLA-DR Expression on Monocytes in Acute Dengue Infection Umi Solekhah Intansari; Muhammad Ilham Bin Azib; Mohd Nazirul Shareef bin Mohd Ridhwan; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Dengue is the most common disease of mosquito-borne infection. According to the World Health Organization, it is estimated that 50 million cases ofdengue infection have been reported annually. Macrophages andmonocyte are thought to play an important role in dengue infection both as primary targets of viral infection and as a source of immunomodulatory cytokines.Whenmonocytes are infectedby dengue viruses, it processed the virus by lyses it and expressed the antigen on its surfacemembrane bind together with HLA class II molecules. HLA-DR-expressing monocytes in acute dengue patientsespecially the intensity is still poorly understood.Measurement of at difference days of infectionwill give an evidence about the role ofmonocytes in dengue pathogenesisObjectives: To observe the kinetics on percentage of HLA-DR expressingmonocytes and themedian of HLA-DR expression intensity in acute dengue infection.Methods: This researchwas an observational type study conducted by cross sectionalmethod towards all the dengue patients in Dr. Sardjito’s General Hospital. Blood samples were drawn from 32 acute dengue infected patients from Day 1 to Day 6th. The HLA-DR expression was measured flow cytometrically using FACS Calibur.Results: The data showed that the mean difference in acute dengue infection from Day 1 to Day 6 is not significant with the p-value larger than 0.05, (p>0.05).Conclusion: The peak level of activated HLA-DR monocyte was at day 2 and then decreasing until day 6. There were no significant changes in percentage of HLA-DR-expressing monocytes from the day 1 to the day 6 and the median intensity of HLA-DR expression in acute dengue infection.Keywords HLA-DR-expressing monocytes, Acute Dengue Infection

THE KINETIC OF ACTIVATED MONOCYTES IN ACUTE PHASE DENGUE INFECTION Umi Solekhah Intansari; Arif Bahiyuddin bin Badaruddin; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introductions: Nowadays dengue infection is at present one of the most common mosquito-borne viral diseases of humans worldwide. Initially, Dengue infections were primarily recorded when they occurred as epidemics in tropical and subtropical countries. Monocyte/macrophage infection is central to the pathogenesis of dengue fever and to the origin of dengue hemorrhagic fever. Increased activation of monocytes and greater numbers of DEN-infected cells were associated with more severe Dengue, implicating a role for monocyte activation in dengue immunopathogenesis. Thus, more knowledge about the relation between numbers of activated monocyte with dengue severities is essential for better understanding regarding this subject.Objectives: Studying the relation between numbers of activated monocyte, marked by HLA-DR marker intensity with Dengue Fever severity throughout the disease course, from day 2 until day 6 of acute dengue fever.Methods: This research is cross sectional observational study. The location in done at Dr. Sardjito Hospital in 6 months time.Results: There is a negative correlation between median number of activated monocyte with dengue severity, on day 3 of acute dengue fever.Conclusion: There was a negative correlation between numbers of activated monocyte marked by HLADR intensity with disease severity on day 3 of acute dengue infection.Keywords: Activated Monocyte; Human Leukocyte Antigen (HLA)-DR intensity; Percentage of Activated Monocyte; Dengue Fever Severity.

The Kinetics of White Blood Cells in Acute Dengue Infection Mohd Nasrul Bin Mohd Ghazali; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 3, No 1 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTIntroduction: Dengue is a mosquito borne viral febrile illness with a high incidence rate of approximately 50 million cases of infection world wide every year. Dengue virus can infect many cells, e.g. monocytes, dendritic cells, Kuppfer cells, B cells including bone marrow and lung. Leukocytes plays an important roles in eliminating dengue virus especially monocytes. However, dengue virus sometimes attack the monocytes and uses them for replication causing monocyte to unde go apoptosis in order to prevent spreading by certain mechanisms.Objectives: To explore the difference in white blood cells count in acute dengue patients from day 2 to day 6.Methods: This research is conducted in a cross sectional observational study method by recording the WBC count, Lymphocytes count, Neutrophils Count, Relative Monocytes Count, and Absolute Monocytes Count from NS-1 positive dengue infection patient using the hematology analyzer. The data was taken from day 2 to day 6 of the fever. One-way ANOVA test was used and a p value <0.05 was considered as significant.Results: In this study, there is a significant difference of leukocyte count, relative and absolute lymphocytes count, relative and absolute neutrophils count, and relative and absolute monocytes count from day 2 to day 6 of dengue infection (p value less than 0.05).Conclusion: Dengue patient have leucopenia on day 2 until day 6 of dengue fever. Lymphocytosis occurs on day 6 of dengue fever. Neutrophils decrease in early infection. Monocytes count is normal in dengue fever, but decrease in DHFKeywords: dengue in fection, leukocyte, lymphocytes, neutrophils, monocytes

The Kinetics of CD8+ T Lymphocytes in Dengue Patients in Yogyakarta Loo Huai Na; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTIntroduction: Dengue fever can be graded into dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The CD8+ T lymphocytes mediate antiviral activity by producing cytokines and directly destroyed the dengue virus infected cells. This study focuses in observing the kinetics of CD8+ T lymphocytes absolute and relative count in dengue patients.Objectives: To observe the kinetics of CD8+ T lymphocytes absolute and relative count in dengue patients.Methods: The research design used is a descriptive study. This research measures and observes the kinetics CD8+ T lymphocytes absolute and relative count from day 2 to day 7. The CD8+ T lymphocytes count was determined using flowcytometry. Data was analyzed using ANOVA and independent t test with p<0.05 considered as significant.Results: The CD8+ T lymphocytes absolute count is low during the beginning of disease course and it gradually increases from day 2 to day 7. The CD8+ T lymphocytes relative count decreases from day 2 to day 3, and start to increase back from day 3 to day 7. There is no difference between the level of CD8+ T lymphocytes absolute count and relative count between DF and DHF patients.Conclusion: There is an increase in CD8+ T lymphocytes absolute count and relative count in dengue patients. There is no difference between DF and DHF patients in CD8+ T lymphocytes absolute and relative count. Keywords: dengue fever; dengue hemorrhagic fever; CD8+ T lymphocytes; absolute count; relative count.

Kinetics of CD69 Expression on Natural Killer Cells During Acute Phase of Dengue Infection` Marshita Binti Kamarudin; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTIntroduction: Dengue infection is major annual public health problem in Indonesia. NK cells have a role in cellular immunity to viral infection, however only a few studies of NK cells and were conducted in vivo especially in Indonesia.Objectives: To explore the kinetics of CD 69 expression on NK cells during the acute phase of dengue infection.Methods: Observational cohort study in Dr. Sardjito Hospital was conducted. Clinical data and laboratory data was collected to measure the number of activated NK cells (CD69) using flowcytometry. The percentage of CD69 then calculated using non- parametric test (Kuskal-Wallis Test) and Student t-test. The fluorescence intensity of CD69 was also analyzed.Results: The mean of activated NK cells (CD69) percentage was higher during the early days of acute phase (day 2 to day3), and continuously declined until the seventh day but statistically they were not significant. Fluorescence intensity of CD69 showed its peak during the fifth day of fever.Conclusion: CD69 expression on activated NK cells were increased during the early days (day 2-day 3) of acute fever but decreased after that (day 4-day 7). The highest intensity of CD69 expression was on the fifth day of fever. Keywords: Dengue infection, kinetics, NK cell, CD69, cellular immune response, acute phase, adults.

Co-Authors Anggy Pangaribuan Antonia Adeleide Anutopi Arguni, Egi Arif Bahiyuddin bin Badaruddin Armytasari, Inggar Deddy Hediyanto Desi Rusmawaningtyas Dibyo Pramono Dominicus Husada Dwiyanti Puspitasari, Dwiyanti Eddy Supriyadi, Eddy Eggi Arguni Egi Arguni Eko Andriyanto Endy Paryanto Prawirohartono Endy Paryanto Prawirohartono Ika Puspitasari Ika Puspitasari Indah Kartika Murni Lea Sutrisna Loo Huai Na Maria Mahdalena Tri Widiyati Marshita Binti Kamarudin Milza Delfina Mohd Nasrul Bin Mohd Ghazali Mohd Nazirul Shareef bin Mohd Ridhwan Muhammad Ilham Bin Azib Novrilia Atika Nabila Patmasari, Linda Ratni Indrawanti Rina Triasih Rizaldy Pinzon Sabrina Handayani Tambun Setya Wandita Siti Nurhayati Tambun, Sabrina Handayani Umi Solekhah Intansari Widya Adhitama Wirawan, Muhammad Taufik Yanri Wijayanti Subronto Yudi Pradipta Zita Dhirani Pramono

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