Article Per Year (5 Year)
p-Index From 2020 - 2025
1.497
P-Index
This Author published in this journals
All Journal Tropical Medicine Journal Sari Pediatri Paediatrica Indonesiana JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Jurnal Gizi Klinik Indonesia Majalah Farmaseutik Medica Hospitalia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Berita Kedokteran Masyarakat
Ida Safitri Laksanawati
Departemen Ilmu Kesehatan Anak, Fakultas Kedokteran, Kesehatan Masyarakat Dan Keperawatan Universitas Gadjah Mada/ RSUP Dr. Sardjito, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Medicine & Pharmacology Nursing Public Health

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 19 Documents
Search

 1   2 
The HLA-DR Expression on Monocytes in Acute Dengue Infection Umi Solekhah Intansari; Muhammad Ilham Bin Azib; Mohd Nazirul Shareef bin Mohd Ridhwan; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.945 KB) | DOI: 10.22146/tmj.4311

Abstract

Introduction: Dengue is the most common disease of mosquito-borne infection. According to the World Health Organization, it is estimated that 50 million cases ofdengue infection have been reported annually. Macrophages andmonocyte are thought to play an important role in dengue infection both as primary targets of viral infection and as a source of immunomodulatory cytokines.Whenmonocytes are infectedby dengue viruses, it processed the virus by lyses it and expressed the antigen on its surfacemembrane bind together with HLA class II molecules. HLA-DR-expressing monocytes in acute dengue patientsespecially the intensity is still poorly understood.Measurement of at difference days of infectionwill give an evidence about the role ofmonocytes in dengue pathogenesisObjectives: To observe the kinetics on percentage of HLA-DR expressingmonocytes and themedian of HLA-DR expression intensity in acute dengue infection.Methods: This researchwas an observational type study conducted by cross sectionalmethod towards all the dengue patients in Dr. Sardjito’s General Hospital. Blood samples were drawn from 32 acute dengue infected patients from Day 1 to Day 6th. The HLA-DR expression was measured flow cytometrically using FACS Calibur.Results: The data showed that the mean difference in acute dengue infection from Day 1 to Day 6 is not significant with the p-value larger than 0.05, (p>0.05).Conclusion: The peak level of activated HLA-DR monocyte was at day 2 and then decreasing until day 6. There were no significant changes in percentage of HLA-DR-expressing monocytes from the day 1 to the day 6 and the median intensity of HLA-DR expression in acute dengue infection.Keywords HLA-DR-expressing monocytes, Acute Dengue Infection
THE KINETIC OF ACTIVATED MONOCYTES IN ACUTE PHASE DENGUE INFECTION Umi Solekhah Intansari; Arif Bahiyuddin bin Badaruddin; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1044.323 KB) | DOI: 10.22146/tmj.4572

Abstract

Introductions: Nowadays dengue infection is at present one of the most common mosquito-borne viral diseases of humans worldwide. Initially, Dengue infections were primarily recorded when they occurred as epidemics in tropical and subtropical countries. Monocyte/macrophage infection is central to the pathogenesis of dengue fever and to the origin of dengue hemorrhagic fever. Increased activation of monocytes and greater numbers of DEN-infected cells were associated with more severe Dengue, implicating a role for monocyte activation in dengue immunopathogenesis. Thus, more knowledge about the relation between numbers of activated monocyte with dengue severities is essential for better understanding regarding this subject.Objectives: Studying the relation between numbers of activated monocyte, marked by HLA-DR marker intensity with Dengue Fever severity throughout the disease course, from day 2 until day 6 of acute dengue fever.Methods: This research is cross sectional observational study. The location in done at Dr. Sardjito Hospital in 6 months time.Results: There is a negative correlation between median number of activated monocyte with dengue severity, on day 3 of acute dengue fever.Conclusion: There was a negative correlation between numbers of activated monocyte marked by HLADR intensity with disease severity on day 3 of acute dengue infection.Keywords: Activated Monocyte; Human Leukocyte Antigen (HLA)-DR intensity; Percentage of Activated Monocyte; Dengue Fever Severity.
The Kinetics of White Blood Cells in Acute Dengue Infection Mohd Nasrul Bin Mohd Ghazali; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 3, No 1 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (963.547 KB) | DOI: 10.22146/tmj.5827

Abstract

ABSTRACTIntroduction: Dengue is a mosquito borne viral febrile illness with a high incidence rate of approximately 50 million cases of infection world wide every year. Dengue virus can infect many cells, e.g. monocytes, dendritic cells, Kuppfer cells, B cells including bone marrow and lung. Leukocytes plays an important roles in eliminating dengue virus especially monocytes. However, dengue virus sometimes attack the monocytes and uses them for replication causing monocyte to unde go apoptosis in order to prevent spreading by certain mechanisms.Objectives: To explore the difference in white blood cells count in acute dengue patients from day 2 to day 6.Methods: This research is conducted in a cross sectional observational study method by recording the WBC count, Lymphocytes count, Neutrophils Count, Relative Monocytes Count, and Absolute Monocytes Count from NS-1 positive dengue infection patient using the hematology analyzer. The data was taken from day 2 to day 6 of the fever. One-way ANOVA test was used and a p value <0.05 was considered as significant.Results: In this study, there is a significant difference of leukocyte count, relative and absolute lymphocytes count, relative and absolute neutrophils count, and relative and absolute monocytes count from day 2 to day 6 of dengue infection (p value less than 0.05).Conclusion: Dengue patient have leucopenia on day 2 until day 6 of dengue fever. Lymphocytosis occurs on day 6 of dengue fever. Neutrophils decrease in early infection. Monocytes count is normal in dengue fever, but decrease in DHFKeywords: dengue in fection, leukocyte, lymphocytes, neutrophils, monocytes
The Kinetics of CD8+ T Lymphocytes in Dengue Patients in Yogyakarta Loo Huai Na; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.663 KB) | DOI: 10.22146/tmj.17127

Abstract

ABSTRACTIntroduction: Dengue    fever can be graded into dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The CD8+ T lymphocytes mediate antiviral activity by producing cytokines and directly destroyed the dengue virus infected cells. This study focuses in observing the kinetics of CD8+ T lymphocytes absolute and relative count in dengue patients.Objectives: To observe the kinetics of CD8+ T lymphocytes absolute and relative              count in dengue patients.Methods: The research design used is a descriptive study. This research measures and observes the kinetics CD8+ T lymphocytes absolute and relative count from day 2 to day 7. The CD8+ T lymphocytes count was determined using flowcytometry. Data was analyzed using ANOVA and independent t test with p<0.05 considered as significant.Results: The CD8+ T lymphocytes absolute count is low during the beginning of disease course and it gradually increases from day 2 to day 7. The CD8+ T lymphocytes relative count decreases from day 2 to day 3, and start to increase back from day 3 to day 7. There is no difference between the level of CD8+ T lymphocytes absolute count and relative count between DF and DHF patients.Conclusion: There is an increase in CD8+ T lymphocytes absolute count and relative count in dengue patients. There is no difference between DF and DHF patients in CD8+ T lymphocytes absolute and relative count. Keywords: dengue fever; dengue hemorrhagic fever; CD8+ T lymphocytes; absolute count; relative count.
Kinetics of CD69 Expression on Natural Killer Cells During Acute Phase of Dengue Infection` Marshita Binti Kamarudin; Umi Solekhah Intansari; Ida Safitri Laksanawati
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.433 KB) | DOI: 10.22146/tmj.17129

Abstract

ABSTRACTIntroduction: Dengue infection is major annual public health problem in Indonesia. NK cells have a role in cellular immunity to viral infection, however only a few studies of NK cells and were conducted in vivo especially in Indonesia.Objectives: To explore the kinetics of CD 69 expression on NK cells during the acute phase of dengue infection.Methods: Observational cohort study in Dr. Sardjito Hospital was conducted. Clinical data and laboratory data was collected to measure the number of activated NK cells (CD69) using flowcytometry. The percentage of CD69 then calculated using non- parametric test (Kuskal-Wallis Test) and Student t-test. The fluorescence intensity of CD69 was also analyzed.Results: The mean of activated NK cells (CD69) percentage was higher during the early days of acute phase (day 2 to day3), and continuously declined until the seventh day but statistically they were not significant. Fluorescence intensity of CD69 showed its peak during the fifth day of fever.Conclusion: CD69 expression on activated NK cells were increased during the early days (day 2-day 3) of acute fever but decreased after that (day 4-day 7). The highest intensity of CD69 expression was on the fifth day of fever. Keywords: Dengue infection, kinetics, NK cell, CD69, cellular immune response, acute phase, adults.
Faktor Risiko Anemia pada Pasien Kusta Anak dalam terapi MDT (Multi Drug Therapy) Milza Delfina; Setya Wandita; Ida Safitri Laksanawati
Sari Pediatri Vol 18, No 5 (2017)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.768 KB) | DOI: 10.14238/sp18.5.2017.363-7

Abstract

Latar belakang. Sepuluh persen kasus kusta baru yang terdeteksi di Indonesia merupakan kusta anak. Pemberian MDT (multi drug therapy) khususnya komponen dapson dapat menimbulkan efek samping anemia hemolitik. Tujuan. Mengetahui prevalensi dan faktor risiko terjadinya anemia pada pasien kusta anak dengan terapi MDT.Metode. Penelitian cross sectional dilakukan di RS Kusta Sitanala Tangerang pada bulan April-Desember 2013. Subyek penelitian adalah pasien kusta anak usia kurang dari 18 tahun yang diambil secara consecutive sampling. Kriteria inklusi adalah semua pasien kusta anak dengan terapi MDT yang setuju dengan informed consent. Terapi kusta selain MDT atau mendapatkan terapi rutin lain, putus obat MDT >6 bulan atau sedang mengalami reaksi kusta tidak diikutsertakan. Kadar hemoglobin diperiksa dengan metode Cyanmethemoglobin. Analisis menggunakan uji chi square dan Fisher’s exact. Hasil. Didapatkan 70 pasien dengan kadar hemoglobin rata-rata 10,8 g/dL pada kelompok anemia, dan 12,5 g/dL tidak anemia. Empat puluh dari pasien tersebut (57,1%) mengalami anemia. Rerata indeks eritrosit menunjukkan anemia normositik normokromik. Pasien yang mendapatkan terapi MDT ≥3 bulan berisiko untuk mengalami anemia (PR: 2,7; IK95%:1,02-7,23). Faktor usia (PR:1,7; IK95%: 0,64-4,35), jenis kelamin (PR:1,3; IK95%: 0,49-3,26), status gizi (PR:1,6; IK95%: 0,57-4,25), dan jenis terapi (PR:0,4; IK95%: 0,04-4,31) bukan merupakan faktor risiko terjadinya anemia.Kesimpulan. Lama terapi ≥3 bulan merupakan faktor risiko anemia pada kusta anak dengan terapi MDT.
Faktor Prognosis Kematian Sindrom Syok Dengue Anggy Pangaribuan; Endy Paryanto Prawirohartono; Ida Safitri Laksanawati
Sari Pediatri Vol 15, No 5 (2014)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp15.5.2014.332-40

Abstract

Latar belakang. Sindrom syok dengue (SSD) merupakan bentuk klinis yang paling berat dari demam berdarah dengue (DBD) dan mempunyai angka kematian yang tinggi. Prediktor kematian pada DSS masih berbeda-beda, sehingga sangat penting untuk meneliti faktor prognosis yang mempengaruhi kematian SSD pada anak.Tujuan. Mengetahui faktor prognosis kematian anak dengan SSD.Metode. Metode penelitian yang digunakan adalah kohort retrospektif. Subyek adalah pasien SSD sesuai kriteria WHO 1997 yang dirawat di Instalasi Kesehatan Anak RSUP Dr. Sardjito dari Januari 2006 – Juli 2012. Faktor prognosis yang diteliti adalah usia, jenis kelamin, status obesitas, tipe infeksi, keterlambatan berobat, manajemen cairan, derajat trombositopenia, koagulopati, perdarahan mayor, prolonged shock, ensefalopati, disfungsi hati, gagal hati fulminan, disseminated intravascular coagulation (DIC), edema paru dan hipoksemia. Analisis regresi Cox digunakan untuk mengetahui kemaknaan faktor prognosis kematian pada DSS.Hasil. Selama periode Januari 2006 – Juli 2012 terdapat 221 pasien DSS dengan angka kematian 27%. Sembilanpuluh enam pasien diikutkan dalam penelitian dan 33(34%) subyek di antaranya meninggal. Analisis multivariat menunjukkan manajemen cairan sebelum masuk rumah sakit Dr. Sardjito yang tidak adekuat (HR 2,658; IK 95% 1,146;6,616), perdarahan mayor (HR 8,223; IK 95% 1,741;38,831) dan prolonged shock (HR 15,805; IK 95% 3,486;71,660) merupakan faktor prognosis independen kematian pada anak dengan SSD.Kesimpulan. Manajemen cairan sebelum masuk rumah sakit rujukan yang tidak adekuat, perdarahan mayor dan prolonged shock merupakan faktor prognosis independen kematian pada anak dengan SSD.
Profil Hematologi Sebagai Prediktor Sepsis pada Sindrom Syok Dengue Deddy Hediyanto; Ida Safitri Laksanawati; Ratni Indrawanti; Eggi Arguni; Desi Rusmawaningtyas
Sari Pediatri Vol 18, No 4 (2016)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp18.4.2016.260-4

Abstract

Latar belakang. Infeksi dengue di daerah endemis dapat terjadi bersamaan dengan infeksi lain. Penelitian tentang infeksi dengue pada anak sudah banyak dilakukan, tetapi masih sedikit yang meneliti tentang kejadian sepsis pada sindrom syok dengue (SSD). Profil hematologi sebagai pemeriksaan yang mudah dilakukan, diharapkan dapat digunakan sebagai prediktor sepsis pada SSD.Tujuan. Mengetahui profil hematologi sebagai prediktor sepsis pada SSD.Metode. Penelitian kohort retrospektif pada anak usia 1 bulan-18 tahun yang diambil dari data rekam medis pasien SSD dengan sepsis maupun tidak sepsis dan dirawat di RSUP Dr. Sardjito mulai 1 Januari 2011- 31 Desember 2014. Profil hematologi dan C-reactive protein (CRP) yang digunakan adalah pemeriksaan yang diambil saat pasien pertama kali masuk ke rumah sakit. Analisis statistik dikerjakan dengan analisis univariat, kemaknaan dengan Odds ratio (OR) dan interval kepercayaan 95% (IK95%).Hasil. Didapatkan 98 pasien yang memenuhi kriteria inklusi SSD, di antaranya 30 pasien SSD (30,6%) menderita sepsis. Tidak ada profil hematologi saat pasien pertama kali masuk rumah sakit menjadi prediktor sepsis pada SSD. Pemeriksaan kadar Hb (rerata 13,98±2,28 g/dL, p=0,897), hematokrit saat datang (rerata 40,5±6,6%; p=0,369), leukosit (median 5,68x 103 sel/µL; p= 0,619), trombosit (median 25,5x103 sel/µL; p=0,841). Pemeriksaan CRP dilakukan pada 40 pasien. Pasien SSD dengan sepsis 57,9% memiliki kadar CRP ≥6 mg/L signifikan lebih tinggi dibanding SSD tanpa sepsis (23,8%) dengan nilai p=0,028; OR 2,1 (IK95%: 1,1-3,9). Pemeriksaan biakan darah dilakukan pada 35 pasien, dengan biakan tumbuh pada 6 pasien DSS yang sepsis. Kesimpulan. Kadar CRP ≥6 mg/L berhubungan signifikan dengan kejadian sepsis pada SSD, sedangkan profil hematologi lain tidak. 
Obesity as a risk factor for dengue shock syndrome in children Maria Mahdalena Tri Widiyati; Ida Safitri Laksanawati; Endy Paryanto Prawirohartono
Paediatrica Indonesiana Vol 53 No 4 (2013): July 2013
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.849 KB) | DOI: 10.14238/pi53.4.2013.187-92

Abstract

Background Dengue hemorrhagic fever (DHF) leads to highmorbidity and mortality if not be treated properly and promptly.Obesity may play a role in the progression ofDHF to dengue shocksyndrome (DSS) and could be a prognostic factor.Objective To evaluate childhood obesity as a prognostic factorfor DSS.Methods We reviewed medical records of patients with DHFand DSS admitted to Department of Child Health, Dr. SardjitoHospital, Yogyakarta between June 2008 and February 2011.Subjects were aged less than 18 years and fulfilled WHO criteria(1997) for DHF or DSS. The exclusion criteria were the denguefever, a milder form of disease, or other viral infections. Riskfactors for DSS were analyzed by logistic regression analysis.Results Of342 patients who met the inclusion criteria, there were116 DSS patients (33 .9%) as the case group and 226 DHF patients(66.1%) as the control group. Univariate analysis revealed thatrisk factors for DSS were obesity (OR= 1.88; 95%CI 1.01 to3.5 l) ,secondary infection type (OR=0.82; 95%CI 0.41 to 1.63), plasmaleakage with hematocrit increase> 25% (OR=3.42; 95%CI 2.06to 5.65), platelet count < 20,000/μL (OR= l.95; 95%CI 1.20 to3 .16), and inadequate fluid management from prior hospitalization(OR=9.ll; 95% CI 1.13 to 73.66). By multivariate analysis,plasma leakage with hematocrit increase > 25% was associatedwith DSS (OR=2.5 l; 95%CI 1.12 to 5.59), while obesity was notassociated with DSS (OR= l.03; 95%CI 0.32 to3.3 1).Conclusion Obesity is not a risk factor for DSS, while plasmaleakage with hematocrit increase > 25% is associated with DSS.
Evaluasi Luaran Klinis Terapi Antibiotik pada Pasien Community Acquired Pneumonia Anak Rawat Inap Sabrina Handayani Tambun; Ika Puspitasari; Ida Safitri Laksanawati
JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Vol 9, No 3
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.91 KB) | DOI: 10.22146/jmpf.47915

Abstract

Community Acquired Pneumonia (CAP) is an infectious disease which is one of the main causes of child mortality in developing countries. The pattern of giving antibiotics at the hospital is usually still empirical. Inappropriate use of antibiotics may cause failure of therapy or bacterial resistance. This study aims to determine the empirical antibiotic rationality and the relationship of rationality to the clinical outcome of CAP-pediatric inpatients at RSUP. Dr. Sardjito Yogyakarta. The study conducted using a descriptive analytic method with a retrospective cohort design. The subjects were CAP-pediatric inpatients at RSUP. Dr. Sardjito Yogyakarta period 1 January-31 December 2018. The rationality of empirical antibiotics is evaluated using the Gyssens algorithm. The clinical outcome was either good or bad outcome according to the clinician stating in the medical record. Patient characteristics, empirical antibiotic therapy and rationality patterns were analyzed descriptively. The relationship between empirical antibiotic rationality and clinical outcome were evaluated using Chi square test. There were 73 patients who met the inclusion and exclusion criteria (132 empirical antibiotic regimens). Rational antibiotic therapy accounted 76.5% (category 0). Types of irrationality of antibiotic found were IIIB (5.3%) and IIA categories (18.2%). Chi-square analysis showed that empirical antibiotic rationality related to good clinical outcome of CAP children (p = 0.011; OR = 2.957; 95% CI = 1,263 - 6,923).
Co-Authors Anggy Pangaribuan Antonia Adeleide Anutopi Arguni, Egi Arif Bahiyuddin bin Badaruddin Deddy Hediyanto Desi Rusmawaningtyas Dibyo Pramono Dominicus Husada Dwiyanti Puspitasari, Dwiyanti Eggi Arguni Egi Arguni Eko Andriyanto Endy Paryanto Prawirohartono Endy Paryanto Prawirohartono Ika Puspitasari Ika Puspitasari Lea Sutrisna Loo Huai Na Maria Mahdalena Tri Widiyati Marshita Binti Kamarudin Milza Delfina Mohd Nasrul Bin Mohd Ghazali Mohd Nazirul Shareef bin Mohd Ridhwan Muhammad Ilham Bin Azib Novrilia Atika Nabila Ratni Indrawanti Rina Triasih Rizaldy Pinzon Sabrina Handayani Tambun Setya Wandita Siti Nurhayati Tambun, Sabrina Handayani Umi Solekhah Intansari Widya Adhitama Yanri Wijayanti Subronto Yudi Pradipta Zita Dhirani Pramono