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All Journal Jurnal Akuakultur Indonesia Jurnal Sain Veteriner JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health)
Putu Eka Sudaryatma
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Agriculture, Biological Sciences & Forestry Public Health Veterinary

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Immunocytochemistry Streptavidin Biotin: Early Detection of Viral Nervous Necrosis Virus in the mucous of the Ikan Kerapu Macan(Epinephelus fuscoguttatus) Putu Eka Sudaryatma; Artanti Tri Lestari; Ni Luh Sunarsih; Ketut Sri Widiarti; Sulis NurHidayah; Didik Srinoto Srinoto
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8307.831 KB) | DOI: 10.22146/jsv.2487

Abstract

Kerapu macan  (Epinephelus fuscoguttatus)    is  one of the  kerapu  fishes  that  has been successfully  bred by the  farmers.  The  survival  rate  of  the  fish  reaches  up  to  the  40%  at  the  fish  hatchery  of  the  home  industry  of  in  Bali. The disease in which may cause significant mortality in kerapu fish, especially for the larvae and juveniles is viral nervous necrosis (VNN). We therefore developed and applied the immunological diagnostic approach of immunocytochemistry technique of streptavidin biotin (SB) for early detection of  VNN. Mucous samples of VNN  infected-  kerapu  fish  in  vivo  were   firstly  detected  by  reverse  transcriptase-polymerase  chain  reaction  (R T -PCR)  for  the  presence  of  DNA  VNN.  If  it  is  RT -PCR-positive  VNN,  the  mucous  samples  were  then  tested  by  SB. Results of the present study indicated that the VNN virus could be detected with SB technique within only 24 hours post infection.  It  was concluded that  rapid  and accurate SB technique  is  suitable and appropriate to  be applied  for  routine  control  and  prevention  national  program  in  the  Fish  Quarantine  for  Indonesia  because  no  need of  fish  sacrification,  and  scientific,  law  and  international    accepted,  and  even  no  hazardous  environmental contamination.Keywords:  kerapu  macan  (Epinephelus  fuscoguttatus),VNN,  mucous,  SB,  early  detection
Physical and molecular identification of fish and shrimp diseases in some farms of West Java, Banten and Jakarta, Indonesia Adam Saba Anggara; Ida Ayu Mirah Meliana Dewi; Kismono Kudoasmoro; Putu Eka Sudaryatma; Putu Angga Wiradana
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.157-167

Abstract

Infectious disease is a major challenge in fish and shrimp farming systems caused by microorganisms including bacteria, viruses and parasites. Physical detection has limitations in detecting diseases in cultivated animals earlier, because of the varied clinical symptoms. The aims of this study was to identify the physical and molecular presence of infectious pathogens in fish and shrimp cultivated in several ponds in West Java, Banten and Jakarta. Monitoring was carried out in two stages, namely the period March–April 2021 and September 2021 by taking samples from several ponds in Bogor, Tangerang, Depok and Jakarta. The sample criteria used were fish and shrimp showing normal and clinical symptoms of being attacked by a disease which were collected by purposive sampling. The collected samples were examined in two stages, namely physical examination of clinical symptoms and molecular examination using PCR. The results showed that of the 24 species samples collected, 17 species showed normal clinical symptoms and seven species showed clinical symptoms. Of the 24 fish and shrimp samples, four samples were infected (2 samples from normal species and 2 samples with clinical symptoms). The infected normal samples were Osphronemus goramy and Litopenaeus vannamei. The infected samples with clinical symptoms were Carassius auratus by Aeromonas salmonicida and red zebra cichlid (Metriaclima estherae) by Red Sea Bream Iridoviral Disease (RSBIV). Physical testing supported by molecular detection of aquatic animals can be an effort to manage aquaculture systems in Indonesia. Keyword: aquaculture, emerging disease, fisheries products, molecular assay ABSTRAK Penyakit infeksi adalah tantangan utama pada sistem budidaya ikan dan udang yang disebabkan oleh mikroorganisme termasuk bakteri, virus, dan parasit. Deteksi secara fisik memiliki keterbatasan dalam mengetahui lebih dini penyakit pada hewan budidaya, karena gejala klinis yang bervariasi. Tujuan dari penelitian ini adalah untuk melakukan identifikasi fisik dan molekuler keberadaan patogen infeksi pada ikan dan udang yang dibudidayakan di beberapa pertambakan di Jawa Barat, Banten, dan Jakarta. Pemantauan dilakukan dalam dua tahap yaitu periode Maret – April 2021 dan September 2021 dengan mengambil sampel dari beberapa pertambakan di Bogor, Tangerang, Depok, dan Jakarta. Kriteria sampel yang digunakan adalah ikan dan udang yang menunjukkan gejala klinis terserang oleh penyakit yang dikumpulkan secara purposive sampling. Sampel yang terkumpul diperiksa dalam dua tahap yaitu pemeriksaan fisik gejala klinis dan pemeriksaan molekuler menggunakan PCR. Hasil menunjukkan dari 24 sampel spesies yang terkumpul, sebanyak 17 spesies menunjukkan gejala klinis normal dan tujuh spesies menunjukkan gejala klinis. Dari 24 sampel ikan dan udang, terdapat empat sampel yang terinfeksi (2 sampel dari spesies normal dan 2 sampel dengan gejala klinis). Sampel normal yang terinfeksi adalah Osphronemus goramy dan Litopenaeus vannamei. Sampel yang terinfeksi dengan gejala klinis adalah Carassius auratus oleh Aeromonas salmonicida dan red zebra cichlid (Metriaclima estherae) oleh Red Sea Bream Iridoviral Disease (RSBIV). Pengujian fisik yang didukung dengan deteksi molekuler pada hewan budidaya dapat menjadi upaya manajemen sistem akuakultur di Indonesia. Kata kunci: akuakultur, emerging disease, perikanan, pengujian molekuler
Occurrence and Consumer Health Risk Assessment of Heavy Metals in Frozen Demersal Fish and Cephalopod Products from Benoa Port, Bali Province Putu Angga Wiradana; I Made Gde Sudyadnyana Sandhika; Putu Eka Sudaryatma; I Gede Widhiantara; Made Nyandra; Adnorita Fandah Oktariani; Setyo Budi Kurniawan
JURNAL KESEHATAN LINGKUNGAN Vol. 16 No. 1 (2024): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v16i1.2024.41-50

Abstract

Introduction: Heavy metal pollution has become an important environmental issue today. This study was aimed to identify the heavy metals of the two fishing products namely demersal fish and cephalopods landed at Benoa Port, Bali Province and their health risk effects on consumers. Methods: Demersal fish and cephalopod products were obtained from local fishermen at Benoa port. Atomic Absorption Spectrophotometry (AAS) was used to specify the (Pb, Cd, and Hg) in 34 fish fillet products weighing an average of 500 grams each. Statistical software was used to conduct an analysis of the collected data, while THQ, TTHQ, and TCR were calculated and compared with USEPA regulations. Results and Discussion: The results of this research showed that the content of heavy metals in demersal fish and cephalopod products was lower than the levels suggested by official agencies in a number of countries. In populations of various ages, the target Hazard Quotient (THQ) for the heavy metal was less than 1. At this point, the Total THQ result did not imply any risk to human health from ingestion of these two fishery products. When these two products were ingested, the target cancer risk (TCR) did not represent a cancer risk. Conclusion: The outcomes of this study showed that the bioaccumulation levels of both fishery products with low levels of heavy metals are safe for human consumption.
The effectiveness of maggot and probiotic feed supplementation on the growth performance and intestinal microbiota of Indonesian shortfin eel, Anguilla bicolor bicolor Sutrisno, Sutrisno; Otie Dylan Soebhakti Hasan; Triyanto , Triyanto; Putu Angga Wiradana; Putu Eka Sudaryatma
Jurnal Akuakultur Indonesia Vol. 25 No. 1 (2026): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.25.1.105-118

Abstract

The purpose of this study was to determine the effectiveness of probiotic and maggot flour supplementation on production performance, water quality, and eel gut microbiota. There were five treatment groups of maggot flour doses (0, 25, 50, 75, and 100%) each with three replications. The eel seeds used were three months old (average weight 6.11 ± 4.28 g, average length 14.3 ± 3.22 cm) as many as 15 fish/aquarium which were fed until full twice a day for 60 days. Growth performance measurements were carried out every two weeks and at the end of the study, survival rate, feed digestibility, water quality, and the abundance of water and eel gut bacteria. The growth rate of group P1 (0% maggot flour) of 1.222 ± 0.662 g/day was significantly higher (P≤0.05) than group P5 (100% maggot flour) of 0.223 ± 0.094 g/day. The best FCR value was shown by treatment P1 of 2.576 ± 0.598. The highest protein digestibility was shown by group P5 at 75.90%, while the lowest protein digestibility was shown by group P1 at 62.41%. Low digestible protein and high fecal protein were shown by group P5 of 15.15% and 28.77%, respectively. The abundance of bacteria in the Bacillus sp. and Lactobacillus sp. groups dominated in groups P2 and P5. Based on the research results, it can be concluded that the use of commercial probiotics and 100% dose of maggot flour is not yet effective in improving growth performance, but can increase the abundance of good bacteria in the intestines of eels. Keywords: Anguilla bicolor, growth parameters, gut microbiota, maggot flour, probiotic   Abstrak Tujuan penelitian ini adalah untuk mengetahui efektivitas suplementasi probiotik dan tepung maggot terhadap performa produksi, kualitas air, dan mikrobiota usus sidat. Terdapat lima kelompok perlakuan dosis tepung maggot (0, 25, 50, 75, dan 100%) masing-masing dengan tiga kali ulangan. Benih sidat yang digunakan berumur tiga bulan (berat rata-rata 6.11 ± 4.28 g, panjang rata-rata 14.3 ± 3.22 cm) sebanyak 15 ekor/akuarium yang diberi pakan sampai kenyang sebanyak dua kali sehari selama 60 hari. Pengukuran performa pertumbuhan dilakukan setiap dua minggu sekali dan akhir penelitian, tingkat kelangsungan hidup, daya cerna pakan, kualitas air, serta kelimpahan air dan bakteri usus belut. Laju pertumbuhan kelompok P1 (0% tepung maggot) sebesar 1,222 ± 0,662 g/hari lebih tinggi secara signifikan (P≤0,05) dibandingkan kelompok P5 (100% tepung maggot) sebesar 0,223 ± 0,094 g/hari. Nilai FCR terbaik ditunjukkan oleh perlakuan P1 sebesar 2,576 ± 0,598. Daya cerna protein tertinggi ditunjukkan oleh kelompok P5 sebesar 75,90%, sedangkan daya cerna protein terendah ditunjukkan oleh kelompok P1 sebesar 62,41%. Protein cerna rendah dan protein feses tinggi ditunjukkan oleh kelompok P5 masing-masing sebesar 15,15% dan 28,77%. Kelimpahan bakteri pada kelompok Bacillus sp. dan Lactobacillus sp. mendominasi pada kelompok P2 dan P5. Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan probiotik komersial dan tepung maggot dosis 100% belum efektif dalam meningkatkan kinerja pertumbuhan, tetapi mampu meningkatkan kelimpahan bakteri baik dalam usus dari ikan sidat. Kata kunci: Anguilla bicolor, mikrobiota usus, parameter pertumbuhan, probiotik, tepung maggot
Co-Authors Adam Saba Anggara Adnorita Fandah Oktariani Artanti Tri Lestari Didik Srinoto Srinoto I Gede Widhiantara I Made Gde Sudyadnyana Sandhika Ida Ayu Mirah Meliana Dewi Ketut Sri Widiarti Kismono Kudoasmoro Made Nyandra Ni Luh Sunarsih Otie Dylan Soebhakti Hasan Putu Angga Wiradana Setyo Budi Kurniawan Sulis NurHidayah Sutrisno, Sutrisno Triyanto , Triyanto