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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal Jurnal Biologi Tropis Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner WIDYA LAKSANA Aquacultura Indonesiana Journal of Aquaculture and Fish Health INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Abdi Insani Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology Jurnal Ilmiah Sains Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Putu Angga Wiradana

Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains, Dan Teknologi, Universitas Dhyana Pura

Author-ID : 2925748

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Biomonitoring Escherichia coli and Coliform Contamination in Abalone (Haliotis squamata) Cultivation Pond in Musi Village, Gerokgak Sub-District, Buleleng-Bali. Wiradana, Putu Angga; Yusup, Deny Suhernawan; Soegianto, Agoes
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Abalone (Haliotis squamata) is one of the fisheries commodities that have high economic value. This study was conducted to investigate contamination of Escherichia coli and coliform in Abalone cultivation ponds in the Musi Village area, Buleleng Regency, Bali. An experiment was carried out by taking water samples in the abalone pond every week for one month. The results showed that there was no difference between the total number of Escherichia coli and coliform. Water samples that were positive for coliform in the determination test had no differences in each sample with total coliform values (Colonies / 100mL) of 4, 3, 7 and 9 (MPN / 100mL) respectively. While the total number of Escherichia coli is shown after being grown in EMBA selective media which are 4, 3, 7, 4 (MPN / 100mL) respectively. Meanwhile, when compared with the number referenced from the Decree of the State Minister of Environment Number 51 of 2004 concerning Sea Water Quality Standards, the quality of seawater for aquatic cultivation in this study is still classified as Safe (<1000 MPN / 100mL).

Steroidogenesis Mechanism, Disruption Factor, Gene Function, and Role in Male Fertility : A Mini Review I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; Putu Angga Wiradana; BagusKomang Satriyasa
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16914

Male fertility can be defined as the ability of the male reproductive system to impregnate a woman, whileinfertility is the condition of a married couple who have been married for one year or more and have had sexualintercourse regularly or without using contraception but do not have a pregnancy or offspring. About 10% ofmarried couples experience infertility. The main organ of male reproduction is the testes because in the testesthe process of forming spermatozoa and the hormone testosterone occurs. The hormone testosterone plays adirect role in the continuity of spermatogenesis. Testosterone is produced through a series of steroidogenesismechanisms in testicular Leydig cells. Several factors influence the course of steroidogenesis such as Leydigcells, steroidogenesis proteins, related genes to the influence of free radicals. These factors are closelyrelated to diet and lifestyle. This study is important to understand in efforts to prevent infertility in men.

Evaluation of antimicrobial activity and phytochemical screening of red Kamboja (Plumeria rubra L.) extracts Ni Kadek Yunita Sari; Ni Wayan Deswiniyanti; Putu Angga Wiradana
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.25409

Natural antimicrobial sources such as red Kamboja (Plumeria rubra L.) flower extract can be utilized to treat infectious disorders caused by Staphylococcus aureus, Escherichia coli, and Candida albicans. This study aims to determine evaluate the effectiveness of methanol and ethanol extracts of P. rubra floral against E. coli, S. aureus, and C. albicans growth, as well as the amount of secondary metabolites in P. rubra extract. The study's findings indicate that the highest DIZ value of P. rubra methanol extract was 7.40 mm, 7.36 mm, and 7.30 mm for S. aureus ATCC25923 at 5%, 10%, and 20%, respectively, while the highest DIZ value for C. albicans ATCC10231 at 10%, 10%, and 20% was 25.08 mm, and 25.04 mm, respectively. The DIZ value of the P. rubra flower ethanol extract against E. coli strain was 5.26 mm at 5%, and 7.30 mm at 20%. Secondary metabolites of saponins, tannins, alkaloids, flavonoids, steroids, and phenols were present in the methanol and ethanol extracts of P. rubra flowers. In summary, our findings highlight the use of P. rubra flower extract as a biological source with antibacterial properties for the control of human infectious illnesses.

Komposisi Floristik Vegetasi di Kawasan Hilir Sungai Unda, Kabupaten Klungkung, Bali I Made Sara Wijana; I Made Saka Wijaya; Abd. Rahman As-syakur; Gede Surya Indrawan; Putu Angga Wiradana; Komang Kartika Indi Swari; Dewa Ayu Intan Tirta Sari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p03

The downstream area of Unda River Watershed located in Klungkung Regency has experienced succession due to the eruption of Mount Agung in 1963. The ecosystem in the downstream area of the Unda River also experienced anthropogenic succession due to human activities which affected the plant species that composing the vegetation. This study aims to observe the floristic composition of vegetation in the downstream area of the Unda River. The research was conducted by exploration method in three types of ecosystems: lowland forest, marshes, and beaches. The results show the floristic composition in the downstream of Unda River composed by 163 plant species, classified in 48 families and 128 genera. The major growth form is small herb (47 species), grass (35 species), and tree (31 species). Fabaceae is the plant family with the most abundant species, followed by Poaceae and Cyperaceae. Based on the research zones, the lowland forest has the highest number of plant species (120 species), while the beach zone has the lowest (82 species). The ecosystem stability in the downstream area of Unda River can be disturbed by the existence of invasive species, such as Eichhornia crassipes, Chromolaena odorata, Lantana camara, Ipomoea carnea, Vachellia farnesiana, and Typha angustifolia.

Checklist, Indeks Ekologi, dan Status Konservasi Komunitas Fauna Akuatik di Kawasan Sungai Unda dan Sekitar Pantai Jumpai, Kabupaten Klungkung, Provinsi Bali Gede Surya Indrawan; Putu Angga Wiradana; I Made Saka Wijaya; Abd. Rahman As-syakur; M. Rheza Rizki Syahputra; I Made Sara Wijana
Bumi Lestari Journal of Environment Vol 21 No 1 (2021)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2021.v21.i01.p02

Aquatic organisms or nekton have an important role in supporting the stability of the aquatic environment and human life. This study aims to determine the nekton type community, ecological index, and their conservation status in the Unda River area and around Jumpai Beach, Klungkung Regency, Bali. The study was conducted in July - August 2020. The study was conducted in 2 communities, namely Macrozoobenthos and Nekton. Macrozoobenthos samples were collected using the quadratic method in the Jumpai Beach area and the direct observation method along the Unda River. Nekton communities are collected through primary data using "survey methods" and secondary data through interviews with fishermen and local communities. The ecological index data were analyzed using the Shannon-Weiner diversity index (H'), dominance index (C), and uniformity index (E), while the data on Nekton type and conservation status were analyzed descriptively. The nekton species community in the waters of the Unda River and Jumpai Beach areas was obtained as many as 26 species in 21 families which were dominated by Kepala Timah (Aplocheilus panchax) and sepat rawa (Trichogaster trichopterus), while Nautica sp for the Macrozoobenthos community. The ecological index of the aquatic community is the Shannon-Weiner Diversity Index (H') value of 2.98 (moderate), the wealth index (R) of 3.95 (moderate), and the evenness index (E) of 1.21. (High). The highest percentage ratio of nekton communities was obtained by the Macrozoobenthos community, namely 53%, while the nekton species was 46%. Conservation status shows that 13 species are included in the LC (Least Concern) category, 12 species are included in the NE (Not Evaluated) category, and 1 species are included in the VU (Vulnerable) category.

Antihypercholesterolemic and Antioxidant Effects of Blumea balsamifera L. Leaf Extracts to Maintain Luteinizing Hormone Secretion in Rats Induced by High-Cholesterol Diets I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; Putu Angga Wiradana; Luh Putu Widiastini; I Made Jawi
The Indonesian Biomedical Journal Vol 13, No 4 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i4.1694

BACKGROUND: The discovery of herbal ingredients for antihypercholesterolemic and to improve male reproductive function is very necessary due to high-cholesterol diet factors. Blumea balsamifera leaf extract (BBLE) is known to be able to increase the number and diameter of Leydig cells in rats given high-fat feed. This study was to conducted to determine the levels of total cholesterol, body weight, plasma malondialdehyde (MDA), Superoxide Dismutase (SOD), Luteinizing Hormone (LH) in high-cholesterol-fed male rats provided with BBLE.METHODS: This research utilized a randomized post-test only control group. Ethanol was used to extract the BBLE, which was then evaporated. For 21 days, 16 Wistar rats were given a high-cholesterol diet. To determine the effect of BBLE on the high-cholesterol diet, the samples were divided into two groups (control and BBLE group) on day 22. The treatments lasted 30 days. SOD, plasma MDA, LH, and total cholesterol were measured.RESULTS: The results showed that the SOD and LH parameters were significantly higher in the treatment of BBLE compared to the control group (p<0.05). The parameters of total cholesterol levels, bodyweight, and MDA of rats given BBLE were significantly lower than those of the control group (p<0.05).CONCLUSION: Our findings highlight that BBLE has antihypercholesterolemic and antioxidant effects. The BBLE also has potential to be used as a therapy to maintain male reproductive function because it has a positive effect on the hypothalamic-pituitary axis through increasing LH secretion.KEYWORDS: Malondialdehyde, Superoxide dismutase, Luteinizing hormone, Blumea balsamifera extract, High-cholesterol diet

First Report on Molecular Identification of Caulerpa Green Algae from Mandangin Island Indonesia Using Partial 18SrRNA Genes Nwet Darli Kyaw Zaw; Putu Angga Wiradana; Sin War Naw; Aondohemba Samuel Nege; Mochammad Amin Alamsjah; Rizhar Eman Karunia Akbar; Fahror Rosi
Journal of Aquaculture and Fish Health Vol. 9 No. 3 (2020): JAFH Vol. 9 No. 3 September 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v9i3.19255

Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.

Supplementation of Lysine Essential Amino Acids in Commercial Feed to Increase Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) in Catfish (Pangasius sp.) Fillet Muhammad Taufiq Shidqi; Agustono Agustono; Mirni Lamid; Putu Angga Wiradana
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2995

The purpose of this study is to see how adding the amino acid lysine to commercial feed affects the amount of EPA and DHA in catfish (Pangasius sp.) flesh. This study is an experimental study with a totally randomized design that includes four treatments and five replications. P0 (100% commercial feed + 1.2 percent amino acid lysine), P2 (100% commercial feed + 2.2 percent amino acid lysine), and P3 (100% commercial feed + 3.2 percent amino acid lysine) were the treatments. The parameters observed were EPA and DHA in catfish meat. Data analysis used ANOVA and continued with the DUNCAN test (p 0.05). The results showed that the addition of the amino acid lysine to commercial feed for 30 days of maintenance was able to increase the EPA and DHA content in catfish meat. The highest EPA content was obtained by treatment P1 (1.2% amino acid lysine), which was 0.597%, while the highest DHA content was obtained by treatment P2 (2.2% amino acid lysine), which was 0.747%. Our findings demonstrate that adding the amino acid lysine to the diet can result in nutrient-rich catfish flesh. However, studies on the influence of lysine amino acid on the growth rate, survival rate, and cholesterol ratio in catfish flesh are predicted to enhance the productivity of this very nutritious fish.

Struktur dan Komposisi Vegetasi pada Suksesi di Muara Sungai Unda, Kabupaten Klungkung, Bali I Made Saka Wijaya; Gede Surya Indrawan; Putu Angga Wiradana; I Made Sara Wijana; Abd. Rahman As-syakur; Adi Ariyanto Wibisono; Viryanando Evan Rahardja
JURNAL ILMIAH SAINS Volume 21 Nomor 1, April 2021
Publisher : Sam Ratulangi University

Â Letusan Gunung Agung pada tahun 1963 memberikan dampak ekologis di sekitar muara Sungai Unda, Kabupaten Klungkung, Bali. Lahar yang mengalir melalui sungai tersebut menyebabkan terjadinya suksesi primer akibat tutupan pasir dan batu. Selama 57 tahun, muara Sungai Unda tidak hanya mengalami suksesi alam, tetapi juga mengalami suksesi antropogenik melalui pemanfaatan sebagai tambang pasir, lahan pastura, dan permukiman sementara.Â Penelitian ini bertujuan untuk mempelajari struktur dan komposisi vegetasi pada suksesi di muara Sungai Unda. Penentuan struktur vegetasi dilakukan pada 8 stasiun penelitian dengan menggunakan metode plot dengan jumlah 24 plot pohon (20 x 20 m) dan 48 plot vegetasi lantai (1 x 1 m). Data dianalisis menggunakan parameter ekologis seperti densitas, densitas relatif, frekuensi, frekuensi relatif, dominansi, dominansi relatif, dan nilai penting, serta dilengkapi dengan indeks keragaman Shannon-Weiner (Hâ€™), indeks dominansi (C), dan indeks keseragaman (E). Hasil penelitian menunjukkan bahwa semak dan rumput merupakan bentuk hidup yang paling menentukan struktur vegetasi di Muara Sungai Unda. Beberapa spesies penyusun vegetasi tersebut merupakan tumbuhan invasif seperti yang harus dikontrol populasinya adalah Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, dan Vachellia farnesiana. Berdasarkan indeks yang digunakan, keanekaragaman vegetasi termasuk dalam kategori tinggi, tanpa ada jenis yang mendominasi, dengan persebaran yang merata.Kata kunci: Gunung Agung; spesies invasive; suksesi antropogenik; vegetasi suksesiStructure and Composition of Successional Vegetation in Unda River, Klungkung Regency, BaliABSTRACTMount Agung eruption in 1963 caused major ecological impact around Unda River, Klungkung Regency, Bali. The volcanic mudflow that surpass the Unda River watershed initiate the primary succession in those areas. This research aimed to study the structure and composition of successional vegetation in Unda River. Twenty-four tree plots (20 x 20 m) and 48 understorey plots (1 x 1 m) derived from 8 research stations were used to determine the structure of vegetation. The data analyzed by ecological parameters, such as density, relative density, frequency, relative frequency, dominancy, relative dominancy, and importance value, then completed with Shannon-Weiner diversity index (Hâ€™), dominancy index (C), and evenness index (E). The results shown that the vegetation structure in Unda River determined by two major growth form: shrubs and grasses. Some notable invasive species that should be controlled are Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, and Vachellia farnesiana. Based on the three indexes, the vegetation diversity was categorized as high diversity, without any species domination, and the distribution of the species are uniformKeywords: Anthropogenic succession; invasive species; Mount Agung; successional vegetation

Potensi Jahe Merah (Zingiber officinale var. rubrum Theilade) sebagai Antimikroba Resisten Ni Kadek Yunita Sari; Ni Komang Widiastuti; Putu Angga Wiradana
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 5 (2022): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (954.868 KB)

ABSTRAKResistensi antimikroba merupakan penurunan kepekaan mikroba terhadap efek obat antimikroba yang menyebabkan proses terapi tidak efektif. Adanya penggunaan antimikroba yang tidak rasional dalam pola peresepan memicu terjadinya resistensi terhadap antimikroba. Untuk mencegah perkembangan resistensi, strategi lain digunakan, yaitu mendapatkan senyawa antibakteri dengan menggunakan komponen bioaktif dari keanekaragaman hayati dan menggunakan bahan-bahan herbal sebagai bahan alternatif obat. Salah satu tanaman obat yang populer digunakan sebagai obat tradisional sekaligus sebagai bahan baku utama jamu adalah jahe merah. jahe merah mengandung senyawa antimikroba golongan fenol, flavonoid, minyak atsiri, dan tannin yang merupakan golongan senyawa bioaktif yang dapat menghambat pertumbuhan mikroba dan dapat dijadikan sebagai antibakteriKata kunci: Resistensi, Jahe Merah, Antimikroba

Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aris Jatmiko Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Panjaitan, Novaria Sari Dewi Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Sugama, I Ketut Surachmi Setiyaningsih Teguh Hari Sucipto, Teguh Hari Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Yesha Ainensis El G. Pelupessy Yohanes Corne

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