Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner
Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Fakultas Kedokteran HEwan Universitas Gadjah Mada
Veterinary

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
THE USE OF DIFFERENT STAINING METHODS TO EVALUATE THE DEVELOPMENT AND VIABILITY OF BOVINE EMBRYOS OBTAINED IN VIVO AND IN VITRO Sri Gustari; Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1037.419 KB) | DOI: 10.22146/jsv.9177

Abstract

In an attempt to find a good method to evaluate embryos we classified the in vitro embryos into freezable and non-freezable by subjective method. These embryos were compared with in vivo freezable embryos using Giemsa staining. The embryo development and viability was evaluated by fluorescent stain (PDA and Hoechst 33342) and compared with the result after Giemsa stain.That number of cells in freezable in vitro and in vivo embryos were not different (P > 0.1), while in vitro fresszable and non-freezable embryos showed significantly differences (P < 0,001) in number of cells.The agreement between Hoechst 33342 and Giemsa stain was quite high (63%) in embryos with number of cells less than 20, and low agreement (25 - 34 %) in embryios with number of cells more than 20. There was a significantly correlation (0.82) betwen the degree of fluorescent of FDA stain and the number of cells at Giemsa stain.The subjective classification of in vitro embryos can be used in the practical situation. PDA staining is a good tool to check this classification as well as to evaluate the development and viability of embroys in different step of experimental trials. Hoechst can be a valuable
Co-Authors Sri Gustari