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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Kedokteran Hewan

Sri Gustari

Departemen Reproduksi Dan Kebidanan Fakultas Kedokteran Hewan Universitas Gadjah Mada

Author-ID : 385557

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Kualitas Morfologi Oosit Sapi Peranakan Ongole yang Dikoleksi secara In Vitro Menggunakan Variasi Waktu Transportasi Agung Budiyanto; Sri Gustari; Dito Anggoro; Dwi Jatmoko; Silvana Nugraheni; Eka Wahyu Nugraha; Donata Asta
Acta VETERINARIA Indonesiana Vol. 1 No. 1 (2013): Januari 2013
Publisher : IPB University

Salah satu alternatif usaha peningkatan populasi sapi di Indonesia adalah dengan transfer embrio. Kualitas oosit yang baik akan menghasilkan tingkat pembelahan dan blastosis yang baik. Lama waktu transportasi dari rumah potong hewan (RPH) ke laboratorium merupakan salah satu faktor yang dilaporkan berpengaruh terhadap kualitas oosit. Waktu transportasi yang tepat untuk menghasilkan oosit dengan kualitas morfologi terbaik belum pernah dilakukan untuk ovarium sapi di Indonesia. Penelitian ini dilakukan untuk mengetahui pengaruh lama waktu transportasi ovarium terhadap kualitas morfologi oosit sapi yang dikoleksi secara in vitro. Koleksi ovarium dilakukan di RPH, selanjutnya dibawa ke laboratorium untuk dilakukan proses koleksi oosit dengan metode aspirasi. Ovarium dikelompokkan berdasarkan waktu transportasinya, yaitu 2, 3, 4, dan 5 jam. Oosit yang diperoleh kemudian dikelompokkan berdasarkan kualitasnya dengan mengklasifikasikan menjadi kualitas A, B, C, dan D. Pada penelitian ini ditemukan adanya perbedaan yang signifikan antara lama waktu transportasi ovarium sapi terhadap kualitas morfologi oosit (P< 0,05). Dari penelitian ini dapat disimpulkan bahwa ovarium yang mengalami perlakuan transportasi selama 2 jam menghasilkan persentase jumlah oosit dengan kualitas morfologi A dan B yang lebih baik jika dibandingkan dengan ovarium yang mengalami perlakuan transportasi selama lebih dari 2 jam.

PENGARUH PEMBERIAN PROSTAGLANDIN F-2a DAN GONADOTROPHIN RELEASING HORMON TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH YANG MENGALAMI KASUS KAWIN BERULANG Surya Agus Prihatno; Sri Gustari
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Pengujian Daya Tahan Spermatozoa Semen Beku Kambing dan Domba setelah Thawing Sri Gustari
Jurnal Sain Veteriner Vol 21, No 1 (2003): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

INTRA-UTERIN ADMINISTRATION OF PROSTAGLANDIN FOR INDUCTION IN CROSS BREEDING GOATS Sri Gustari; Asmarani Kusumawati; Slamet Subagyo; Prabowo P Putro
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8622

Productivity and reproductivity of cross breeding goats is low. It is mainly caused by hormone deficiency and difficulties in estrus detection. The research studied the effect of intrauterine administration of prostagiandin on estrus induction and on number of pregnancies.Sixteen does were divided randomly into 2 groups. Group i were injected with prostagiandin intramuscularly. Group li were given prostagiandin by intrauterine administration. In both groups, prostagiandin were given twice with interval 11 days apart. The does which coming into estrus were mated naturally.There was a significantly different in the time of estrus between group I and group II (40.25 ± 4.94 and 80.8 ± 18.35 hours). On the other hand, there was no difference in estrus induction between those two groups (Group I : 87.5% and Group II : 62.5 %). All the does which were mated naturally were pregnant (100%). Although time to estrus after intrauterine administration of prostagfandin was longer than that of intramuscular ones, but it was a cheaper method.

THE USE OF DIFFERENT STAINING METHODS TO EVALUATE THE DEVELOPMENT AND VIABILITY OF BOVINE EMBRYOS OBTAINED IN VIVO AND IN VITRO Sri Gustari; Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In an attempt to find a good method to evaluate embryos we classified the in vitro embryos into freezable and non-freezable by subjective method. These embryos were compared with in vivo freezable embryos using Giemsa staining. The embryo development and viability was evaluated by fluorescent stain (PDA and Hoechst 33342) and compared with the result after Giemsa stain.That number of cells in freezable in vitro and in vivo embryos were not different (P > 0.1), while in vitro fresszable and non-freezable embryos showed significantly differences (P < 0,001) in number of cells.The agreement between Hoechst 33342 and Giemsa stain was quite high (63%) in embryos with number of cells less than 20, and low agreement (25 - 34 %) in embryios with number of cells more than 20. There was a significantly correlation (0.82) betwen the degree of fluorescent of FDA stain and the number of cells at Giemsa stain.The subjective classification of in vitro embryos can be used in the practical situation. PDA staining is a good tool to check this classification as well as to evaluate the development and viability of embroys in different step of experimental trials. Hoechst can be a valuable

Pengaruh Pemberian GnRH pada Sapi Potong yang Mengalami Kawin Berulang (Repeat Breeding) Surya Agus Prihatno; Sri Gustari; Asmarani Kusumawati; Agung Budiyanto; Erif Maha Nugraha Setyawan; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62761

Repeat breeding is a condition in which cows have normal estrous cycles and have been mated 3 times or more but are not pregnant. The incidence of repeat breeding in beef cattle can be detrimental to breeders in terms of time, that the calving period becomes longer so that the cows cannot give birth to calves once a year. Various ways to deal with repeat breeding have been done, but the results are not optimal. This study was conducted to determine the effect of Gonadotrophin Releasing Hormone (GnRH) in cows with repeat breeding. A total of fifteen beef cattle with repeat breeding were used in this study. The cows were divided into 3 groups of 5 each. Cows that were estrus in the first group (as a control group) were only performed artificial insemination (AI) without GnRH injection. In the second and third groups, after AI, they were injected with GnRH at AI time and 3-4 days after AI with a dose of 5 ml (Fertagyl®, 500 µg gonadorelin) intramuscularly. A pregnancy examination was carried out three months later and the results were recorded. The data obtained were analyzed descriptively and quantitatively using Chi-Square statistical analysis. The results of the analysis showed that there were differences in the pregnancy rate in the first group (control) with the second cow group and the third cow group, which were 20%, 80%, and 60% respectively. Based on statistical analysis, there was a significant difference in pregnancy rate (P <0.05) between the three groups. It was concluded that GnRH administration could increase the pregnancy rate in beef cattle that had repeat breeding.

TEPUNG BEKICOT (Achatina fulica) SEBAGAI PAKAN TAMBAHAN UNTUK MENINGKATKAN KUALITAS SEMEN DOMBA (Ovis aries) Jayanti Berliana Dewi; Delvi Ramadayani; Marchellia Dhiafifah Wahyunandha; Melati Kusuma Bunda Pertiwi; Sri Gustari; Topas Wicaksono Priyo Jr
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69114

One of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increase

Konstruksi Plasmid pET-15b dengan Gen tat Virus Penyakit Jembrana Asmarani Kusumawati; Lalu Unsunnidhal; Agung Budiyanto; Erif Maha Nugraha Setyawan; Sri Gustari
Jurnal Sain Veteriner Vol 41, No 1 (2023): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.79217

Virus Penyakit Jembrana adalah virus yang menyebabkan jembrana pada sapi bali. Vaksin protein rekombinan dibuat dengan memasukkan DNA atau gen yang mengkode protein imunogenik ke dalam vektor ekspresi prokariotik sehingga dapat mengekspresikan protein rekombinan yang akan dijadikan vaksin. Salah satu gen struktural virus penyakit jembrana adalah gen tat yang mengekspresikan protein tat yang digunakan sebagai vaksin protein rekombinan. Tujuan dari penelitian ini adalah untuk mengkloning pET15b dengan gen tat. Konfirmasi gen pET-15b pada bakteri transforman dilakukan dengan koloni PCR, kemudian dilakukan isolasi plasmid dan dilaksanakan konfirmasi menggunakan metode PCR dengan primer untuk deteksi gen tat dan primer sekuensing untuk vektor pET15b. Hasilnya menunjukkan bahwa bakteri transforman sudah berisikan DNA rekombinan pET15b-tat yang terkonfirmasi melalui koloni PCR dengan band sebesar 179 bp, kemudian dikonfirmasi lanjut melalui PCR pada hasil isolasi plasmid dari bakteri transforman yang didapatkan band sebesar 179 bp untuk primer deteksi gen tat dan 514 bp untuk primer sekuensing pada vektor pET15b, 179 bp dan 514 bp sesuai dengan target amplikasi untuk primer deteksi dan primer sekuensing yang digunakan.

PENGARUH PEMBERIAN MINYAK JINTAN HITAM (Nigella sativa L.) TERHADAP PENINGKATAN SPERMATOGENESIS TIKUS WISTAR YANG TERPAPAR ASAP ROKOK Mujahidatul Musfiroh; Sri Gustari
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui efek minyak jintan hitam (Nigella sativa L.) terhadap spermatogenesis tikus Wistar yang terpapar asap rokok. Jenis penelitian adalah eksperimental murni dengan subyek 24 ekor tikus yang terpapar asap rokok selama 21 hari dan dirandomisasi dalam 4 kelompok yaitu kelompok kontrol dan kelompok perlakuan dengan pemberian minyak jintan masing-masing 0,1; 0,2; dan 0,3 ml. Penegakan hipotesis dilakukan dengan menggunakan Krusskal-Wallis dan Mann Whitney dengan batas derajat kemaknaan P≤0,05, interval kepercayaan 95%. Pemberian minyak jintan hitam dapat memperbaiki derajat spermatogenesis.

Co-Authors Agung Budiyanto Agung Budiyanto Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bayu Sulistyo Delvi Ramadayani Dito Anggoro Donata Asta Dwi Jatmoko Eka Wahyu Nugraha Erif Maha Nugraha Setyawan Erif Maha Nugraha Setyawan Ian Kurniawan Jayanti Berliana Dewi Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome Lalu Unsunnidhal Marchellia Dhiafifah Wahyunandha Melati Kusuma Bunda Pertiwi Mujahidatul Musfiroh Ni Wayan Kurniani Karja Prabowo P Putro Silvana Nugraheni Slamet Subagyo Surya Agus Prihatno Topas Wicaksono Priyo Jr Veronika Gilang Pravitasari Winny Plumeria Aqshani Yesi Pratiwi Kusumawati Yosua Kristian Adi Yuke Rizky Amelia

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