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Asmarani Kusumawati
Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
Veterinary

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Kombinasi One-step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dan Nucleic Acid Lateral Flow (NALF) sebagai Metode Deteksi Gen env-tm Virus Jembrana Strain Tabanan 1987 Asmarani Kusumawati; Fatimah Fatimah
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8202.514 KB) | DOI: 10.22146/jsv.41146

Abstract

Jembrana disease is an acute viral disease  in Bali cattle that have short incubation period, high mortality and morbidity rate. Early diagnostic methods are needed  to prevent the further spreading of this disease. In this study, we combined One-step RT-PCR  and  NALF  methods to detect env-tm gene of Jembrana virus Tabanan 1987 strain. Viral RNA isolated from a spleen of the infected cattle was used as the template. One-step RT-PCR procedure was performed using oligoprobes  labeled digoxigenin and  right primer sequence that were designed using the primer3plus program based  on the conserves region of env-tm gene from NCBI database. The product of one-step RT-PCR was tested using NALF  method  instead of  electrophoresis. Positive result was shown by the appearance of dark lines on the test line of digoxigenin in NALF dipstick device.
Pengaruh Ukuran Ovarium dan Folikel Terhadap Penampilan Reproduksi pada Sapi Po dan Simpo di Kecamatan Jatinom, Kabupaten Klaten Topas Wicaksono Priyo Jr; Agung Budiyanto; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.43960

Abstract

The increasing beef cattle population in Indonesia is not significantly with high demand of meat every year. The insignificant increasing in population is caused by fertility decreasing, infectious disease and the reduction amount of forage land. The factor which causes cattle fertility decreasing can’t be separated from the ovarian and follicular diameter which have an effect on high various reproductive appearance. Parameters that use for knowing reproductive appearance of cattle are Service per Conception (S/C) and Calving Interval (CI). The aims of this study is to describe ovary sizes and follicular diameter in cattle, to describe ovary and follicular diameter toward S/C and CI variation. This research uses female cattle, 8 SimmentalPO (SimPO) and 9 Ongole breeds (PO), multiparous, are not pregnant, 4-8 years old, Body Condition Score (BCS) 2.5-3.5, peak phase estrus, has no reproductive problems, clear recordings and live in the area of farmer in Jatinom District, Klaten Regency, Central Java Province. Cattle are examined for ovarian size and follicle size using ultrasonography. S/C and CI data were obtained from records of artificial insemination cards (AI). The data obtained were analyzed by t test. The results of this study showed there were no differences in the diameter of ovaries in the size of SimPO and PO cattle (P> 0.05), there were no differences in the diameter of SimPO and PO cattle follicles (> 0.05), there were differences in ovarian and follicular diameter sizes with respect to S/C and CI in cattle with good and bad reproductive performance (P <0.05). The conclusion of the study showed that there was no difference in ovarian and follicular diameter size in SimPO and PO cattle (P> 0.05) but there was an influence on S/C and CI (P <0.05).
Kerusakan Deoxyribonucleic Acid (DNA) Spermatozoa Memengaruhi Tingkat Kebuntingan Sapi Brahman (DAMAGE TO DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA AFFECTING THE LEVEL OF PREGNANCY IN BRAHMAN CATTLE) Langgeng Priyanto; Agung Budiyanto; Asmarani Kusumawati; Kurniasih Kurniasih
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.284 KB) | DOI: 10.19087/jveteriner.2019.20.1.119

Abstract

The relationship among of sperm DNA damage in cows with pregnancy rates has not been widely studied. The purpose of this study to determine the relationship of sperm DNA damage with pregnancy rate on Brahman cows. The sperm DNA damage rate was measured by Sperm-BosHalomax® from 2 samples of male Brahman bull straw (40002 and 40885) and pregnancy rate was measured from the success rate of artificial insemination. In 14 female Brahman cows divided into two groups. One group of 7 in the artificial insemination with 40002 males with 37.11% sperm DNA damage and one in artificial insemination with 40885 with 10.65% sperm DNA damage. The data obtained were analyzed descriptively by comparing sperm DNA damage with pregnancy rate. The results showed that at 37.11% sperm DNA damage level was found pregnancy rate 57.11% with ultrasound on 30 day and pregnancy rate 42.80% with ultrasound to 45 day. Result of research on sperm DNA damage level of 10.66% found pregnancy rate 57.11% with ultrasound to 30 day and level pregnancy 57.11% with ultrasound 45 days. The results of this study have concluded that there is a difference in the rate of sperm DNA damage with pregnancy rate in Brahman cows. The sperm DNA damage has an effect on pregnancy rate on Brahman cows.
Co-Authors Agung Budiyanto Fatimah Fatimah Kurniasih Kurniasih Langgeng Priyanto Topas Wicaksono Priyo Jr