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All Journal Buletin Peternakan Agroland : Jurnal Ilmu-Ilmu Pertanian
Kustono Kustono
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Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon Dewi Pranatasari; Kustono Kustono; Diah Tri Widayati
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9080

Abstract

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.
KARAKTERISTIK HABITAT MIKRO SEBAGAI DASAR POLA PENETASAN TELUR MALEO DI TAMAN NASIONAL LORE LINDU SULAWESI TENGAH Hafsah Hafsah; Tri Yuwanta; Kustono Kustono; Djuwantoko Djuwantoko
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 15, No 3 (2008)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.366 KB)

Abstract

The Objective of the study was to identify microhabitat characteristics in natural nesting ground as a basic form of egg hatching of maleo birds for ex-situ habitat in Lore Lindu National Park. Survey methods and experimental design were applied in this study using Saluki Nesting Ground as a habitat model. In a natural habitat, variables observed were microclimate condition (temperature and humidity, depth and diameter of holes) while in an artificial habitat where four different forms of eggs hatching (in-situ, ex-situ, incubator by means of media rack and sand) were applied temperature, humidity, hatching quality, incubation time, hatching weigh, and mortality were determined. Data analysis applied was descriptive analysis and multivariate test using SPSS computer program. Results of the study showed that in the natural nesting ground the temperature ranged from 30 to 35 oC with an average temperature of 33.03oC, and the humidity from 60.20 to 71.10% with an average humidity of 68.55%. The hole diameter ranged from 28-63 cm with an average hole diameter of 41.64 cm and the hole depth from 51-87cm with an average hole depth of 63.29 cm. The application of hatching method was resulted in the in-situ temperature and humidity of 32.90 oC and 68.41%, respectively, and the ex-situ of 31.68 oC and 67.52%, respectively, rack incubator of 33.99 oC and 70.06%, respectively, and sand incubator of 33.78 oC and 71.02%, respectively. The longest incubation time was found in the ex-situ semi natural hatchery which was 78.40 days, followed by the in-situ habitat 63.48 days, rack incubator 59.40 days, and sand incubator 59.33 days. The best hatching quality was found in the rack incubator, but the mortality rate was highest. The application of incubator for egg hatching of maleo birds could reduce the incubation time length and increase the hatching quality
Co-Authors Dewi Pranatasari Diah Tri Widayati Djuwantoko Djuwantoko Hafsah Hafsah Tri Yuwanta