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PENGARUH DOSIS SPERMA YANG DIENCERKAN DENGAN NaCl FISIOLOGIS TERHADAP FERTILITAS TELUR PADA INSEMINASI BUATAN AYAM KAMPUNG Widya Asmarawati; Kustono (Kustono); Diah Tri Widayati; Sigit Bintara; Ismaya (Ismaya)
Buletin Peternakan Vol 37, No 1 (2013): Buletin Peternakan Vol. 37 (1) Februari 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i1.1952

Penelitian ini bertujuan untuk mengetahui pengaruh dosis sperma untuk Inseminasi Buatan (IB) pada ayam kampung terhadap fertilitas telurnya. Dosis IB ini berguna untuk mengetahui perbandingan jantan dan betina yang paling efisien dalam suatu pemeliharaan. Sperma ditampung dan dicampur dari 5 ekor ayam Pelung jantan yang berumur sekitar 52 minggu. Sperma yang telah diketahui konsentrasinya kemudian dibagi menjadi 3 bagian dan masing-masing diencerkan dengan NaCl fisiologis sehingga diperoleh dosis akhir yaitu 25x106/0,1 ml (dosis 1); 75x106/0,1 ml (dosis 2); dan 150x106/0,1 ml (dosis 3). Sembilan ekor ayam kampung betina diinseminasi untuk mengetahui pengaruh dosis terhadap fertilitas telur. Ayam betina yang digunakan adalah ayam kampung yang berumur sekitar 30 minggu. Hasil penelitian menunjukkan bahwa perlakuan dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung. Fertilitas telur untuk masing-masing perlakuan dosis 1, 2, dan 3 adalah 44,13±20,84; 66,67±23,57; dan 58,75±14,36%. Kesimpulan dari penelitian ini adalah dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung.(Kata kunci: Ayam kampung, Dosis sperma, Inseminasi buatan)

PENGARUH PENGENCERAN SPERMA DENGAN AIR KELAPA DAN ARAS KUNING TELUR ITIK SERTA LAMA PENYIMPANAN TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA GARUT PADA PENYIMPANAN 5°C Novia Dimar Dwitarizki; Ismaya (Ismaya); Widya Asmarawati
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v39i3.7979

The Effect of Age on the Quality of Semen Turkeys (Meleagris gallopavo) Riyan Nugroho Aji; Ismaya Ismaya; Sri Harimurti; Heru Sasongko; Sigit Bintara; Widya Asmarawati
Buletin Peternakan Vol 42, No 4 (2018): BULETIN PETERNAKAN VOL. 42 (4) NOVEMBER 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i4.31372

The purpose of this study was to observe the macroscopic and microscopic quality of Bronze turkey fresh semen on various ages. The observed toms were at the ages of 9 to 10 months (P1), 13 to 14 months (P2), and 17 to 18 months (P3), with each taken from 3 different toms. The collection was done by abdominal massage, and collected for 3 times a week in triplicate. This data was analyzed by analysis of variance (ANOVA) in a completely randomized design, followed by Duncan's New Multiple Range Test (DMRT) if there was any difference. The results showed that the average volume of turkey fresh semen were P1 (0.16 ± 0.04 ml), P2 (0.13 ± 0.02 ml), and P3 (0.10 ± 0.02 ml). The average pH of fresh semen were P1 (7.19 ± 0.05), P2 (7.12 ± 0.10), and P3 (7.06 ± 0.06). The average motility percentage of fresh semen were P1 (78.11 ± 1.38%), P2 (82.55 ± 1.17%), and P3 (74.55 ± 4.16%). The average viability percentage of fresh semen were P1 (78.63 ± 1.55%), P2 (83.26 ± 1.54%), and P3 (79.20 ± 1.06%). The average abnormal percentage of spermatozoa were P1 (3.01 ± 0.04%), P2 (3.04 ± 0.88%), and P3 (3.21 ± 0.44%). In conclusion, the best fresh semen quality is found on toms aged from 13 to14 months (P2). Furthermore, at the age 17 to18 months and more, the semen quality was decreased.

The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours Sigit Bintara; Diah Tri Widayati; Widya Asmarawati; Riyan Nugroho Aji
JURNAL ILMIAH PETERNAKAN TERPADU Vol 11, No 1 (2023)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v11i1.p34-40

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.

ESTROGEN HORMONE PROFILE AND ESTRUS RESPONSE OF THIN TAILED EWES SYNCHRONIZED WITH CONTROLLED INTERNAL DRUG RELEASE Seraphina Kumala; widya Asmarawati; Ismaya Ismaya; sigit bintara; Riyan Nugroho Aji; Diah Tri Widayati
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

This research was conducted to identify the estrogen hormone profile and estrus response in the thin tailed ewes synchronized with controlled internal drug release (CIDR) implant. This research was carried out by employing 8 thin tailed ewes, CIDR, and estrogen kit. The ewes were distributed into: 3 ewes as control group and 5 ewes as treated group (with 12 days-CIDR implantation). Ewes were raised in the Sidomukti farm group, Sleman Regency, Yogyakarta. The data obtained were estrogen hormone profile during estrus examined by using ELISA method and estrus response marked with the reddening and oedema of vulva, mucus vaginal discharge, behavioral changes, vaginal pH, and superficial cells population. The data were analyzed by applying independent sample T-test. The result did not show any significant difference (P0.05) in the normal range between the control and CIDR group in estrogen hormone profile, estrus response, and pH level, but showed significant differences with positive correlation (P0.05) in population of superficial cells. In conclusion, CIDR was an effective tool for estrus synchronization at the farmer group, and gave positive estrus response towards thin tailed ewes with estrogen hormone profile, pH level, and superficial cell percentage on the normal range.

ESTROGEN HORMONE PROFILE AND ESTRUS RESPONSE OF THIN TAILED EWES SYNCHRONIZED WITH CONTROLLED INTERNAL DRUG RELEASE Seraphina Kumala; widya Asmarawati; Ismaya Ismaya; sigit bintara; Riyan Nugroho Aji; Diah Tri Widayati
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.18583

This research was conducted to identify the estrogen hormone profile and estrus response in the thin tailed ewes synchronized with controlled internal drug release (CIDR) implant. This research was carried out by employing 8 thin tailed ewes, CIDR, and estrogen kit. The ewes were distributed into: 3 ewes as control group and 5 ewes as treated group (with 12 days-CIDR implantation). Ewes were raised in the Sidomukti farm group, Sleman Regency, Yogyakarta. The data obtained were estrogen hormone profile during estrus examined by using ELISA method and estrus response marked with the reddening and oedema of vulva, mucus vaginal discharge, behavioral changes, vaginal pH, and superficial cells population. The data were analyzed by applying independent sample T-test. The result did not show any significant difference (P0.05) in the normal range between the control and CIDR group in estrogen hormone profile, estrus response, and pH level, but showed significant differences with positive correlation (P0.05) in population of superficial cells. In conclusion, CIDR was an effective tool for estrus synchronization at the farmer group, and gave positive estrus response towards thin tailed ewes with estrogen hormone profile, pH level, and superficial cell percentage on the normal range.

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