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Panjisakti Basunanda
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Studi Keragaman Genetik Dua Puluh Galur Inbred Jagung Manis Generasi S7 Purwito Djoko Yuwono; Rudi Hari Murti; Panjisakti Basunanda
Jurnal Ilmu Pertanian Vol 18, No 3 (2015): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.915 KB) | DOI: 10.22146/ipas.7919

Abstract

Penelitian ini mengevaluasi keragaman genetik dua puluh galur inbred jagung manis populasi S7.  Tujuan penelitian ini adalah untuk mendapatkan informasi keragaman genetik dua puluh galur inbred, heritabilitas dalam arti luas, korelasi genetik dan analisis lintasan, dan jarak genetik antar galur inbred jagung manis. Percobaan lapangan menggunakan rancangan acak kelompok lengkap dengan perlakuan dua puluh galur inbred dan tiga ulangan. Analisis data menggunakan ANOVA, korelasi genetik, dan analisis klaster. Koefisien keragaman genetik yang diperoleh mengindikasikan adanya keragaman genetik antar galur yang diuji. Heritabilitas menunjukkan nilai yang tinggi pada semua variabel kecuali jumlah baris biji. Karakter panjang tongkol dan diameter tongkol memiliki korelasi dan pengaruh langsung yang tinggi terhadap bobot tongkol. Analisis klaster menghasilkan tiga kelompok pada nilai koefisien similarity 70%. Kelompok yang memiliki koefisien similarity paling rendah memiliki jarak genetik terjauh dan dianjurkan sebagai tetua untuk pembuatan hibrida dengan heterosis tinggi.
Studi Keragaman Genetik Dua Puluh Galur Inbred Jagung Manis Generasi S7 Purwito Djoko Yuwono; Rudi Hari Murti; Panjisakti Basunanda
Jurnal Ilmu Pertanian Vol 18, No 3 (2015): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.7919

Abstract

Penelitian ini mengevaluasi keragaman genetik dua puluh galur inbred jagung manis populasi S7.  Tujuan penelitian ini adalah untuk mendapatkan informasi keragaman genetik dua puluh galur inbred, heritabilitas dalam arti luas, korelasi genetik dan analisis lintasan, dan jarak genetik antar galur inbred jagung manis. Percobaan lapangan menggunakan rancangan acak kelompok lengkap dengan perlakuan dua puluh galur inbred dan tiga ulangan. Analisis data menggunakan ANOVA, korelasi genetik, dan analisis klaster. Koefisien keragaman genetik yang diperoleh mengindikasikan adanya keragaman genetik antar galur yang diuji. Heritabilitas menunjukkan nilai yang tinggi pada semua variabel kecuali jumlah baris biji. Karakter panjang tongkol dan diameter tongkol memiliki korelasi dan pengaruh langsung yang tinggi terhadap bobot tongkol. Analisis klaster menghasilkan tiga kelompok pada nilai koefisien similarity 70%. Kelompok yang memiliki koefisien similarity paling rendah memiliki jarak genetik terjauh dan dianjurkan sebagai tetua untuk pembuatan hibrida dengan heterosis tinggi.
Cell wall modifications lead to cultivar differences in apple (Malus domestica) fruit mealiness Ayu Nurlaila Indah; Shigeki Moriya; Tomoya Ohta; Rani Agustina Wulandari; Panjisakti Basunanda; Hideki Murayama
Jurnal Ilmu Pertanian Vol 7, No 2 (2022): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.72768

Abstract

Recently, four quantitative trait loci linked to flesh mealiness in apples were identified, with one associated with the MdPG1 allele. Hence, this study analyzed cell wall changes in two mealy (Orin and Akane) and three non-mealy (Kiou, Kitaro, Fuji) apple cultivars during ripening. The fruits were harvested for each cultivar at optimum maturity and stored at 20°C for 20 days. The flesh firmness of ‘Kitaro’ and ‘Fuji’ fruit did not change strikingly over the 20 days, whereas that of the other three cultivars, especially ‘Akane’ and ‘Orin’, gradually decreased during ripening. Between the two cultivars with a mealy texture, ‘Akane’ fruit produced extremely low levels of ethylene, whereas ‘Orin’ fruit produced high levels. The water-soluble polyuronide (WSP) contents of ‘Kiou’ and ‘Fuji’ fruit did not change clearly. In contrast, the WSP contents of the other three cultivars, especially ‘Akane’ and ‘Orin’, increased during ripening. In ‘Kiou’, ‘Kitaro’, and ‘Fuji’ fruit, the molecular-mass distributions of WSPs did not change during ripening. Conversely, the molecular-mass distribution of WSPs in ‘Akane’ and ‘Orin’ fruit exhibited downshifts during ripening. These results indicate that solubilization and depolymerization of pectic polyuronides occur during ripening in mealy ‘Akane’ and ‘Orin’ fruit, and that ethylene may not be involved in these changes.
Cell wall modifications lead to cultivar differences in apple (Malus domestica) fruit mealiness Ayu Nurlaila Indah; Shigeki Moriya; Tomoya Ohta; Rani Agustina Wulandari; Panjisakti Basunanda; Hideki Murayama
Jurnal Ilmu Pertanian Vol 7, No 2 (2022): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.72768

Abstract

Recently, four quantitative trait loci linked to flesh mealiness in apples were identified, with one associated with the MdPG1 allele. Hence, this study analyzed cell wall changes in two mealy (Orin and Akane) and three non-mealy (Kiou, Kitaro, Fuji) apple cultivars during ripening. The fruits were harvested for each cultivar at optimum maturity and stored at 20°C for 20 days. The flesh firmness of ‘Kitaro’ and ‘Fuji’ fruit did not change strikingly over the 20 days, whereas that of the other three cultivars, especially ‘Akane’ and ‘Orin’, gradually decreased during ripening. Between the two cultivars with a mealy texture, ‘Akane’ fruit produced extremely low levels of ethylene, whereas ‘Orin’ fruit produced high levels. The water-soluble polyuronide (WSP) contents of ‘Kiou’ and ‘Fuji’ fruit did not change clearly. In contrast, the WSP contents of the other three cultivars, especially ‘Akane’ and ‘Orin’, increased during ripening. In ‘Kiou’, ‘Kitaro’, and ‘Fuji’ fruit, the molecular-mass distributions of WSPs did not change during ripening. Conversely, the molecular-mass distribution of WSPs in ‘Akane’ and ‘Orin’ fruit exhibited downshifts during ripening. These results indicate that solubilization and depolymerization of pectic polyuronides occur during ripening in mealy ‘Akane’ and ‘Orin’ fruit, and that ethylene may not be involved in these changes.
Co-Authors Ayu Nurlaila Indah Hideki Murayama Purwito Djoko Yuwono Rani Agustina Wulandari Rudi Hari Murti Shigeki Moriya Tomoya Ohta